Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 218548585

GI number: 218548585

Start: 1259402

End: 1260844

Strand: Reverse

Name: pykA [H]

Synonym: EFER_1218

Alternate gene names: 218548585

Gene position: 1260844-1259402 (Counterclockwise)

Preceding gene: 218548586

Following gene: 218548581

Centisome position: 27.48

GC content: 51.28

Gene sequence:

>1443_bases
ATGTCCAGAAGGCTTCGCAGAACCAAAATCGTTACCACGTTAGGCCCGGCAACTGACCGCGATAACAACCTTGAAAAAAT
TATCGCTGCAGGCGCCAACGTTGTACGTATGAACTTCTCTCACGGATCACCTGAAGATCACAAAATGCGTGCTGATAAGG
TTCGTGAGATCGCAGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCAAAAATCCGTGTATCCACC
TTTAAAGAAGGGAAAGTTTTCCTCAATATTGGCGACAAATTCCTGCTCGATGCCAACCTGGGGAAAGGTGAAGGCGATAA
AGAGAAAGTTGGTATTGACTATAAAGGCCTGCCTGCTGACGTCGTACCAGGCGATATCCTGTTGCTTGACGATGGCCGTG
TACAGTTAAAAGTACTGGAAGTGCAGGATATGAAAGTGTTCACCGAAGTCACGGTTGGTGGTCCACTTTCTAACAATAAA
GGGATCAACAAACTTGGTGGTGGTCTCTCCGCGGAAGCGCTGACTGAAAAAGACAAAGCCGATATCCAGACTGCAGCCCT
GATTGGCGTTGACTATCTGGCGGTTTCATTCCCACGTTGTGGTGAAGATCTGAACTATGCACGCCGTCTGGCTCGTGACG
CTGGTTGCGATGCAAAAATTGTCGCCAAAGTTGAGCGTGCCGAAGCGGTATGCAGCCAGGATGCGATGGATGACATCATT
CTGGCTTCTGACGTAGTCATGGTGGCTCGTGGTGACCTGGGCGTTGAAATTGGCGATCCTGAACTGGTCGGTATTCAGAA
AGCGTTGATTCGTCGGGCGCGTCAACTTAACCGTGCGGTGATTACGGCTACGCAGATGATGGAGTCGATGATCACTAACC
CGATGCCAACACGTGCAGAAGTCATGGACGTCGCAAACGCAGTACTGGATGGCACCGATGCCGTTATGCTTTCAGCAGAA
ACGGCCGCAGGTCAGTATCCGGCAGAAACCGTAGCGGCAATGGCACGCGTTTGCCTGGGCGCAGAAAAAATCCCGAGCAT
CAACGTTTCTAAACACCGTCTGGACGTCCAATTCGACAATGTGGAAGAAGCAATTGCCATGTCCGCAATGTACGCGGCTA
ACCACCTGAAAGGCGTTACTGCGATCATTACCATGACTGAGTCTGGACGTACTGCGTTAATGACGTCTCGTATCAGCTCC
GGCTTGCCGATTTTCGCCATGTCGCGCCATGAACGCACGCTGAACCTGACTGCATTGTACCGTGGTGTAACACCGGTACA
CTTCGACAGCGCCACTGATGGTGTAGCTGCCGCAAGTGAAGCAGTCAATCTGCTTCGCGATAAAGGTTATCTGGTTTCTG
GTGATCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCAACTAATACCACGCGTATTCTGACTGTCGAG
TAA

Upstream 100 bases:

>100_bases
CTTTTCGTCATCCGGCGTGTTCATTTACCCCACGTGAAGTTGGGGAACACCCGGATCAATATTCACGCAACACCTAGTTG
TTCAGTCAACGGAGTATTAC

Downstream 100 bases:

>100_bases
ATGAAGTGCCGGATGCAGCGTAAACCTCATCCGGCCTATAAAAGTATGCAAATTCAAAACATTGCAGGAACACTCTGACC
CAATAGGGCGTAGCGCATCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQDMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSATDGVAAASEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQDMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSATDGVAAASEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTF
KEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQDMKVFTEVTVGGPLSNNKG
INKLGGGLSAEALTEKDKADIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDIIL
ASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSG
LPIFAMSRHERTLNLTALYRGVTPVHFDSATDGVAAASEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=36.6533864541833, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=36.6533864541833, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=36.7676767676768, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=35.2226720647773, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=35.2226720647773, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=35.5172413793103, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=35.5172413793103, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.4728033472803, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.4728033472803, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.0232558139535, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=98.75, Blast_Score=959, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=487, Percent_Identity=36.7556468172485, Blast_Score=277, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=36.40081799591, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=37.8542510121458, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=37.8542510121458, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=37.8542510121458, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=37.8542510121458, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=36.530612244898, Blast_Score=257, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=497, Percent_Identity=35.2112676056338, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.246963562753, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.246963562753, Blast_Score=264, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=32.5865580448065, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24648966, Length=418, Percent_Identity=35.6459330143541, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=35.6617647058824, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51369; Mature: 51238

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHKMRADKVREIAAK
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKVLEVQDMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHH
DIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEE
RGVTPVHFDSATDGVAAASEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE
CCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRMNFSHGSPEDHKMRADKVREIAAK
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKVLEVQDMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEEECCEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHH
DIQTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEE
RGVTPVHFDSATDGVAAASEAVNLLRDKGYLVSGDLVIVTQGDVMSTVGSTNTTRILTVE
CCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]