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The map label for this gene is flk [H]

Identifier: 218548224

GI number: 218548224

Start: 859049

End: 860056

Strand: Reverse

Name: flk [H]

Synonym: EFER_0842

Alternate gene names: 218548224

Gene position: 860056-859049 (Counterclockwise)

Preceding gene: 218548241

Following gene: 218548221

Centisome position: 18.74

GC content: 49.4

Gene sequence:

>1008_bases
GTGATGATGCAACCTATTTCCGGTACTCCACCACGCCCACCGGATATCAACCAAAATCAACCAGCCGGGCCAGGCGAACA
ACCTTTATCAACACAGCAACGCACTGTACTGGAACGTCTTATAACCCGCCTGACTGCATTAACGAAGCAGCAAAGTGCTG
AAGTATGGGCTGCTGTTAAGCATGATCTTGGTATGAAAAGTGATACTCCGTTGCTCTCACGTCATTTTCCCGCTGCGGAA
GTTAATCTTAACCAGCGCCTGCTGCGTGCTCAGCAAACTCACGTGACCCGCCAATTGTTGTCACAACTGAGTGGGCTGCT
CAGTCAGGGGAATAATCGCCAGGCTGTCAGCGATTTCATTCGCCAGCAATACGGTCAAACCGCGCTGAATCAGCTAACTC
CTGAACAGTTAAAAAATGTCCTTACGTTGCTCCAACAAGGCAAACTGGCAATCCCGCAACCACAGCAACGCACACCAACT
GCTCGCCCGTTGTTACCTGCTGAGCATAATACGTTAAAGCAACTGGTGACTAAACTTGCTGCCGCGACCGGGGAGCCAAG
TAAACAGATCTGGCAATCGATGCTGGAGCTTTCGGGGGTGAAGAACGGTGAACTTATCCCGGCGAAACAATTTACCCATC
TTGTGACATGGCTACAGGCTCGTCAGACTTTGAGTTTGCAAAGTGCACCAACGTTGGAAACGTTGCAAATGGCACTGAAG
CAGCCGCTGGAACGGGCTGAACTCACTGAACTGCAACATTATGCACAGCAAACCTGGAATGTCCTGCCGCAAATGGCGCT
CACGTCATCCCAGGTTCTTGATCTGCTTAATCACGTGTTTTTAAAACGTGCGGAGCGAGAAAGTGGATTTATCGATCCGC
GTAATATTCAGCCATTATTTACCCCTTTCCCTCCACTGATGGATACGTTTAAATCCTTCACCGGGAGTACCGGGTTGGTT
TTAATGGCCTTCTTAATTGTGCTCCTGCTTATCTGGTTGGTGATTTAA

Upstream 100 bases:

>100_bases
TCACGTTTGTGTTACCTGTTTGAGAACGAGAGTTAACCGGGCAAGTGTGCCATAATCTCGCCGTCAGGCATACTTACGAA
GATTTATGATTTAAGGATAA

Downstream 100 bases:

>100_bases
CCACGCCGAAAAGAGAGCAGCGTAACCACTATTCCTACAACCGCAGATATCGCCCCGGCAAGAAAAACCGACGGGTAGCC
AAATGAGGTCGCCAGCATTC

Product: flagella biosynthesis regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MMMQPISGTPPRPPDINQNQPAGPGEQPLSTQQRTVLERLITRLTALTKQQSAEVWAAVKHDLGMKSDTPLLSRHFPAAE
VNLNQRLLRAQQTHVTRQLLSQLSGLLSQGNNRQAVSDFIRQQYGQTALNQLTPEQLKNVLTLLQQGKLAIPQPQQRTPT
ARPLLPAEHNTLKQLVTKLAAATGEPSKQIWQSMLELSGVKNGELIPAKQFTHLVTWLQARQTLSLQSAPTLETLQMALK
QPLERAELTELQHYAQQTWNVLPQMALTSSQVLDLLNHVFLKRAERESGFIDPRNIQPLFTPFPPLMDTFKSFTGSTGLV
LMAFLIVLLLIWLVI

Sequences:

>Translated_335_residues
MMMQPISGTPPRPPDINQNQPAGPGEQPLSTQQRTVLERLITRLTALTKQQSAEVWAAVKHDLGMKSDTPLLSRHFPAAE
VNLNQRLLRAQQTHVTRQLLSQLSGLLSQGNNRQAVSDFIRQQYGQTALNQLTPEQLKNVLTLLQQGKLAIPQPQQRTPT
ARPLLPAEHNTLKQLVTKLAAATGEPSKQIWQSMLELSGVKNGELIPAKQFTHLVTWLQARQTLSLQSAPTLETLQMALK
QPLERAELTELQHYAQQTWNVLPQMALTSSQVLDLLNHVFLKRAERESGFIDPRNIQPLFTPFPPLMDTFKSFTGSTGLV
LMAFLIVLLLIWLVI
>Mature_335_residues
MMMQPISGTPPRPPDINQNQPAGPGEQPLSTQQRTVLERLITRLTALTKQQSAEVWAAVKHDLGMKSDTPLLSRHFPAAE
VNLNQRLLRAQQTHVTRQLLSQLSGLLSQGNNRQAVSDFIRQQYGQTALNQLTPEQLKNVLTLLQQGKLAIPQPQQRTPT
ARPLLPAEHNTLKQLVTKLAAATGEPSKQIWQSMLELSGVKNGELIPAKQFTHLVTWLQARQTLSLQSAPTLETLQMALK
QPLERAELTELQHYAQQTWNVLPQMALTSSQVLDLLNHVFLKRAERESGFIDPRNIQPLFTPFPPLMDTFKSFTGSTGLV
LMAFLIVLLLIWLVI

Specific function: Acts as a regulator of flagellar gene expression by modulating the protein level of the anti sigma factor flgM upon sensing ring completion or hook elongation. Flk could inhibit flgM secretion by acting as a braking system for the flagellar- associated ty

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flk family [H]

Homologues:

Organism=Escherichia coli, GI1788661, Length=334, Percent_Identity=62.874251497006, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37529; Mature: 37529

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMQPISGTPPRPPDINQNQPAGPGEQPLSTQQRTVLERLITRLTALTKQQSAEVWAAVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDLGMKSDTPLLSRHFPAAEVNLNQRLLRAQQTHVTRQLLSQLSGLLSQGNNRQAVSDFI
HHCCCCCCCCHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RQQYGQTALNQLTPEQLKNVLTLLQQGKLAIPQPQQRTPTARPLLPAEHNTLKQLVTKLA
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AATGEPSKQIWQSMLELSGVKNGELIPAKQFTHLVTWLQARQTLSLQSAPTLETLQMALK
HHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QPLERAELTELQHYAQQTWNVLPQMALTSSQVLDLLNHVFLKRAERESGFIDPRNIQPLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TPFPPLMDTFKSFTGSTGLVLMAFLIVLLLIWLVI
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MMMQPISGTPPRPPDINQNQPAGPGEQPLSTQQRTVLERLITRLTALTKQQSAEVWAAVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDLGMKSDTPLLSRHFPAAEVNLNQRLLRAQQTHVTRQLLSQLSGLLSQGNNRQAVSDFI
HHCCCCCCCCHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RQQYGQTALNQLTPEQLKNVLTLLQQGKLAIPQPQQRTPTARPLLPAEHNTLKQLVTKLA
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AATGEPSKQIWQSMLELSGVKNGELIPAKQFTHLVTWLQARQTLSLQSAPTLETLQMALK
HHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QPLERAELTELQHYAQQTWNVLPQMALTSSQVLDLLNHVFLKRAERESGFIDPRNIQPLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TPFPPLMDTFKSFTGSTGLVLMAFLIVLLLIWLVI
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9079927; 11677609 [H]