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The map label for this gene is mnmC [H]

Identifier: 218548221

GI number: 218548221

Start: 854256

End: 856262

Strand: Reverse

Name: mnmC [H]

Synonym: EFER_0839

Alternate gene names: 218548221

Gene position: 856262-854256 (Counterclockwise)

Preceding gene: 218548224

Following gene: 218548214

Centisome position: 18.66

GC content: 51.37

Gene sequence:

>2007_bases
GTGAAACACTACGCCATACAACCTGCCAATCTTGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGACGT
TTACTTTTCCAATGACAATGGACTGGAAGAGACACGCTATGTCTTTCTTGGGGGTAACCAATTAGGAGCACGCTTTCCTG
AGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTT
GATCAGTTTCGCGAAGGTCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACTCG
TGCGGATTTAGGATTAGCGCATCAACACTGGCCAGAACTGGCACCGTGGGCAGAAAAACTTCAGGCGCAGTGGCCAATGC
CCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGTGACATTAACGAACTG
ACAAGTCAACTGGACGATTCGCTAAATCAAAAAGTAGACGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGA
TATGTGGACGCAAAATCTGTTTAACACAATGGCAAGGCTGGCGCGTCCTGGCGGCACACTGGCAACGTTTACGTCTGCCG
GTTTTGTCCGCCGTGGTTTGCAGGAAGCCGGATTTACCATGCAAAAGCGCAAAGGGTTTGGGCGTAAACGGGAAATGCTT
TGTGGGGTGATGGAACAGACATTACCCCTCCCCTGCTCCACGCCGTGGTTTAACCGTACGGGTAGTCACAAACGGGAAGC
GGCGATTATCGGCGGTGGTATCGCCAGCGCATTGTTGTCGCTGGCGCTATTACGTCGTGGATGGCAGGTAACGCTTTATT
GTGCTGATGAGGCCCCCGCAATGGGTGCTTCCGGCAATCGCCAGGGGGCACTGTATCCGCTCTTAAGTAAACACGATGAG
GCGTTGAACCGCTTTTTCTCTAATGCGTTCACATTTGCTCGTCGGCTTTACGATTTATTGCCCGTCAAATTTGATCATGA
CTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAACCAGCATAAAATCGCACAGATGTTGTCGATGGATTTACCCG
AAGAACTGGCTGTAGCCGTTGAGGCTAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGTATTACTTAT
CCGCAAGGTGGATGGCTGTGTCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTCA
TTATCAATATCAGTTAAAGGATTTATCCCGTCAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACAC
ACAGTGTAGTGGTACTGGCGAACGGGCATCAAATCAGCGGTTTCAGCCAAACGTCGTCTCTGCCGATGTATTCCGTTGCC
GGTCAAGTCAGCCATATTCCAACAACGCCGGAACTGGCGAAGCTGAAGCAAGTGCTATGCTATGACGGTTATCTCACGCC
ACAAAATCCGGCGAATCAGCACCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGATGAAG
ATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCGCAGTGGGCAAAAGAGGTTGATGTCAGTAAGAAA
GAAGCGCGCTGCGGTGTGCGTTGTGCCACTCGCGATCATCTGCCAATGGTAGGCAATGCCCCCGATTATGAGGCAACGCT
CGTGGAATATACCTCGCTGACAGAACAGCAAGACAACGCGGTAAGCGCACCGGTTTATGACGATCTCTTTATGCTTGCTG
CTTTGGGTTCGCGTGGTTTGTGCTCTGCCCCGCTGTGCGCCGAAATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCG
ATGGATGCCAATACGCTGGCGGCGTTAAATCCAAATCGGTTATGGGTGCGGAAGTTGTTGAAAGGGAAAGCGGTTAAGGC
GGGGTAA

Upstream 100 bases:

>100_bases
GTACGCCGAACAAGTCCGATCAGACGATTAATAGAGAAATTTGCGCAGCCTGACACACATCGTTAAGATCGTCAGACTGC
CCGCCAGACGAGTAACTTAC

Downstream 100 bases:

>100_bases
TCTGCTTTGGCATTGCTTGCCGGATATGGCGTGAACGCCTTATCCGGCACATTACTTACTGCTGTGAAGCCTGTTGAAAC
AGGTTTTCCCACATATCGGT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLGARFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREGHPQAQLQRLHFISFEKFPLTRADLGLAHQHWPELAPWAEKLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNTMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSTPWFNRTGSHKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPAMGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRLYDLLPVKFDHDWCGVTQLGWDEKNQHKIAQMLSMDLPEELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIHYQYQLKDLSRQDDCWLLNFAGDQQATHSVVVLANGHQISGFSQTSSLPMYSVA
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSDEDQQQNRQRLIDCFPQAQWAKEVDVSKK
EARCGVRCATRDHLPMVGNAPDYEATLVEYTSLTEQQDNAVSAPVYDDLFMLAALGSRGLCSAPLCAEILAAQMSDEPIP
MDANTLAALNPNRLWVRKLLKGKAVKAG

Sequences:

>Translated_668_residues
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLGARFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREGHPQAQLQRLHFISFEKFPLTRADLGLAHQHWPELAPWAEKLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNTMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSTPWFNRTGSHKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPAMGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRLYDLLPVKFDHDWCGVTQLGWDEKNQHKIAQMLSMDLPEELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIHYQYQLKDLSRQDDCWLLNFAGDQQATHSVVVLANGHQISGFSQTSSLPMYSVA
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSDEDQQQNRQRLIDCFPQAQWAKEVDVSKK
EARCGVRCATRDHLPMVGNAPDYEATLVEYTSLTEQQDNAVSAPVYDDLFMLAALGSRGLCSAPLCAEILAAQMSDEPIP
MDANTLAALNPNRLWVRKLLKGKAVKAG
>Mature_668_residues
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLGARFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREGHPQAQLQRLHFISFEKFPLTRADLGLAHQHWPELAPWAEKLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNTMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSTPWFNRTGSHKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPAMGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRLYDLLPVKFDHDWCGVTQLGWDEKNQHKIAQMLSMDLPEELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIHYQYQLKDLSRQDDCWLLNFAGDQQATHSVVVLANGHQISGFSQTSSLPMYSVA
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSDEDQQQNRQRLIDCFPQAQWAKEVDVSKK
EARCGVRCATRDHLPMVGNAPDYEATLVEYTSLTEQQDNAVSAPVYDDLFMLAALGSRGLCSAPLCAEILAAQMSDEPIP
MDANTLAALNPNRLWVRKLLKGKAVKAG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=94.9101796407186, Blast_Score=1311, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 74401; Mature: 74401

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLGARFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCHHCCCCCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREGHPQAQLQRLHFISFEKFPLTRADLGLAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCHHHHCCHHHHCCCC
APWAEKLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
APAKNPDMWTQNLFNTMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
CGVMEQTLPLPCSTPWFNRTGSHKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
MGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRLYDLLPVKFDHDWCGVTQLGWDEKN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCH
QHKIAQMLSMDLPEELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCHHHHEEHHHHHHHHHHCCEECCCCCCCCCCCEEEHHHHHHHHHHHHH
QQGLQIHYQYQLKDLSRQDDCWLLNFAGDQQATHSVVVLANGHQISGFSQTSSLPMYSVA
HCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCCCHHHHC
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSDEDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHH
RLIDCFPQAQWAKEVDVSKKEARCGVRCATRDHLPMVGNAPDYEATLVEYTSLTEQQDNA
HHHHHCCCHHHHHHHCCCHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
VSAPVYDDLFMLAALGSRGLCSAPLCAEILAAQMSDEPIPMDANTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC
>Mature Secondary Structure
MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLGARFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCHHCCCCCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREGHPQAQLQRLHFISFEKFPLTRADLGLAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCHHHHCCHHHHCCCC
APWAEKLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
APAKNPDMWTQNLFNTMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
CGVMEQTLPLPCSTPWFNRTGSHKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
MGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRLYDLLPVKFDHDWCGVTQLGWDEKN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCH
QHKIAQMLSMDLPEELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCHHHHEEHHHHHHHHHHCCEECCCCCCCCCCCEEEHHHHHHHHHHHHH
QQGLQIHYQYQLKDLSRQDDCWLLNFAGDQQATHSVVVLANGHQISGFSQTSSLPMYSVA
HCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCCCHHHHC
GQVSHIPTTPELAKLKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSDEDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHH
RLIDCFPQAQWAKEVDVSKKEARCGVRCATRDHLPMVGNAPDYEATLVEYTSLTEQQDNA
HHHHHCCCHHHHHHHCCCHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
VSAPVYDDLFMLAALGSRGLCSAPLCAEILAAQMSDEPIPMDANTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA