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The map label for this gene is hyfR [H]

Identifier: 218548075

GI number: 218548075

Start: 700303

End: 702315

Strand: Reverse

Name: hyfR [H]

Synonym: EFER_0684

Alternate gene names: 218548075

Gene position: 702315-700303 (Counterclockwise)

Preceding gene: 218548076

Following gene: 218548073

Centisome position: 15.31

GC content: 49.33

Gene sequence:

>2013_bases
ATGACAAAATTTGACGAGGCGATGCTCGCCCCGCCGGAAGATAACATGATGGCAGAAACGATCAGTGGCTTGTTACAGCG
TCTGACGCCGCAAAGTGGCAAAGAATCATTATTACAAGCCTTTATGCCACTTTTACCGCCTTTTAGCGCGGTGCAGCTCA
TTGAACTGCATTTTCCTGGAAGCCGCGTCTGGTATCGCTGTTTCGAAGAGGGCATAAGCGAAACCGCTGCCGGAGGTTAT
CAGGGAACCGTTGTCGATTGTTCGCTCCATGCTGTATTACTGAGCGACATGTTAATGGTTGAGATTCGTTTTATTCGCAC
CAAAGGAAGTAAGTTCTCGGTGCAGGATAGCTCACTTTTCAACTGGTTAGTGCGCATTATGACACCAGTGCTGGAGTCTC
TACTGGGGCAGGCGGAACAGCAGGCAACACTGCACACACTGATTAAAGAACGTGATCATCATCGGGTGCTGGTGGATATC
ACCAATGCAGTGCTCACACACCGCGACCGGGATGAATTAATTGCTGATGTCACACGCGAGATCCATCATTTCTTCGGCAT
CACCTCAGTAGGTATGGTGCTGCACGATAATCGCCCTTCCGCAGGCTTTGTCCTCGATATTACCCATTTCTCCCCTACAC
CTGCAGAGCGTTTGCAACGTCCGCTGGCGGTGGAAAGCGCATTAATGCAGCGTATATCCGGGAGTAATCAACACGCATTA
CTTCACCAGGCAGAAGATCCCCTTTTGTGGCAGCACGATCCTTTATTACAGGATTTGCATTCCTCCGGGCTTTCCTGCGC
ATTACTGTTACCCCTTACCTTTGGTCGTCATACTTCCGGGTTGTTGCTTTTGGCTCACAGTTCATCGTGTTTGTTTAGCG
ATGAAAATTGCACGCTTTTGCAACATATCGCTAACCGGATTGCCATTGCGGTCGATAATGCCGATGCGTGGCATAACATG
AACGATCTGAAAGAGCAGTTAAAGCAAGAAAATAACCAACTTAACGAACAGTTACACTGCTCTCAACACGTCGGCGATAT
CATTTATCAAAGCCAGGTGATGGAAGAGTTACTCCAGCAAGTGGGAATTGTGGCGCAAAGTGACAGTACCGTCCTGCTTT
GCGGTGAAACAGGTACCGGAAAAGAGGTGATTGCGCGTACCATCCACCAGCTCAGTCTGCGCCATGACAAACCGCTGGTG
AAAATTAACTGCGCAGCCATTCCTGCTGCACTGCTGGAAAGCGAGTTGTTTGGTCATGACAAAGGGGCGTTTACCGGGGC
TATTAATACTCATCGCGGTCGCTTTGAAATTGCTGATGGCGGGACGCTATTTCTTGATGAGATTGGCGATCTACCGCTGG
AGTTACAACCAAAGCTATTACGAGTATTACAGGAAAAAGAAATTGAACGACTAGGAGGAAACCGCACCATTCCGGTAAAC
GTGCGAGTCATTGCCGCCACTAACCGCGATTTATGGCAGATGGTGGAAGATCGTCAGTTCCGTAGTGATCTGTTTTATCG
GTTGAACGTATTTCCTCTGGAATTGCCACCACTGCGTGATCGACCGGAAGATATTCCCCTTCTGGCGAAATATTTCACGC
AAAAAATGGCGCGTAGTATGAAACGCAAAATTGATGCCATCTCAACCGACACCCTGCGCCAGCTGATGTCATGGGAATGG
CCGGGTAATGTGCGGGAGCTGGAGAATGTGATTGAACGTGCTGTTTTGCTTACCCGCGGTGGTAGTCTCAATCTTCATCT
TCATCCACGACAAACGCGCTTGCTACCTGCGATGAGTGAAAACAGTTTTACTCAAGGACAAATGGCGCAAATGTTTAATC
CTGCTACGCCTGAAAATGATGAAGAAGAACGGCTACGGATTATTCAGGTATTGCGCGAGACAAACGGTATTGTGGCAGGG
CCTCGTGGCGCGGCAACGCGTCTGGGGATGAAACGCACCACGTTGTTGTCCCGAATGCAGCGCCTTGGGATCGCAGTACG
TGAAGTGCTTTAG

Upstream 100 bases:

>100_bases
GGAAGCGACTGAGTGACCAGATGATGAAGCTTCTGGACGATATCGCCCATGAGCCAGCGATCTATTTGCTGGCAAGGAAA
ATAACAGGATCAGGGATACG

Downstream 100 bases:

>100_bases
AAAATTGGCCCGATGAAACATTCACCGGGCCCTGAGATTAACTGTCGCTAACCTGCCCGTCAGGCCAGGCATGAATGACA
GCTTTGATTAACGTTGCCAG

Product: hydrogenase-4 transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTKFDEAMLAPPEDNMMAETISGLLQRLTPQSGKESLLQAFMPLLPPFSAVQLIELHFPGSRVWYRCFEEGISETAAGGY
QGTVVDCSLHAVLLSDMLMVEIRFIRTKGSKFSVQDSSLFNWLVRIMTPVLESLLGQAEQQATLHTLIKERDHHRVLVDI
TNAVLTHRDRDELIADVTREIHHFFGITSVGMVLHDNRPSAGFVLDITHFSPTPAERLQRPLAVESALMQRISGSNQHAL
LHQAEDPLLWQHDPLLQDLHSSGLSCALLLPLTFGRHTSGLLLLAHSSSCLFSDENCTLLQHIANRIAIAVDNADAWHNM
NDLKEQLKQENNQLNEQLHCSQHVGDIIYQSQVMEELLQQVGIVAQSDSTVLLCGETGTGKEVIARTIHQLSLRHDKPLV
KINCAAIPAALLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEKEIERLGGNRTIPVN
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKYFTQKMARSMKRKIDAISTDTLRQLMSWEW
PGNVRELENVIERAVLLTRGGSLNLHLHPRQTRLLPAMSENSFTQGQMAQMFNPATPENDEEERLRIIQVLRETNGIVAG
PRGAATRLGMKRTTLLSRMQRLGIAVREVL

Sequences:

>Translated_670_residues
MTKFDEAMLAPPEDNMMAETISGLLQRLTPQSGKESLLQAFMPLLPPFSAVQLIELHFPGSRVWYRCFEEGISETAAGGY
QGTVVDCSLHAVLLSDMLMVEIRFIRTKGSKFSVQDSSLFNWLVRIMTPVLESLLGQAEQQATLHTLIKERDHHRVLVDI
TNAVLTHRDRDELIADVTREIHHFFGITSVGMVLHDNRPSAGFVLDITHFSPTPAERLQRPLAVESALMQRISGSNQHAL
LHQAEDPLLWQHDPLLQDLHSSGLSCALLLPLTFGRHTSGLLLLAHSSSCLFSDENCTLLQHIANRIAIAVDNADAWHNM
NDLKEQLKQENNQLNEQLHCSQHVGDIIYQSQVMEELLQQVGIVAQSDSTVLLCGETGTGKEVIARTIHQLSLRHDKPLV
KINCAAIPAALLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEKEIERLGGNRTIPVN
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKYFTQKMARSMKRKIDAISTDTLRQLMSWEW
PGNVRELENVIERAVLLTRGGSLNLHLHPRQTRLLPAMSENSFTQGQMAQMFNPATPENDEEERLRIIQVLRETNGIVAG
PRGAATRLGMKRTTLLSRMQRLGIAVREVL
>Mature_669_residues
TKFDEAMLAPPEDNMMAETISGLLQRLTPQSGKESLLQAFMPLLPPFSAVQLIELHFPGSRVWYRCFEEGISETAAGGYQ
GTVVDCSLHAVLLSDMLMVEIRFIRTKGSKFSVQDSSLFNWLVRIMTPVLESLLGQAEQQATLHTLIKERDHHRVLVDIT
NAVLTHRDRDELIADVTREIHHFFGITSVGMVLHDNRPSAGFVLDITHFSPTPAERLQRPLAVESALMQRISGSNQHALL
HQAEDPLLWQHDPLLQDLHSSGLSCALLLPLTFGRHTSGLLLLAHSSSCLFSDENCTLLQHIANRIAIAVDNADAWHNMN
DLKEQLKQENNQLNEQLHCSQHVGDIIYQSQVMEELLQQVGIVAQSDSTVLLCGETGTGKEVIARTIHQLSLRHDKPLVK
INCAAIPAALLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEKEIERLGGNRTIPVNV
RVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKYFTQKMARSMKRKIDAISTDTLRQLMSWEWP
GNVRELENVIERAVLLTRGGSLNLHLHPRQTRLLPAMSENSFTQGQMAQMFNPATPENDEEERLRIIQVLRETNGIVAGP
RGAATRLGMKRTTLLSRMQRLGIAVREVL

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=671, Percent_Identity=71.9821162444113, Blast_Score=953, Evalue=0.0,
Organism=Escherichia coli, GI1789087, Length=609, Percent_Identity=47.4548440065681, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1788550, Length=345, Percent_Identity=46.3768115942029, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI1790437, Length=316, Percent_Identity=46.5189873417722, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=54.7413793103448, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=53.8135593220339, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1790299, Length=431, Percent_Identity=37.122969837587, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1789233, Length=242, Percent_Identity=49.1735537190083, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=48.3050847457627, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=40.8955223880597, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1787583, Length=322, Percent_Identity=36.0248447204969, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI87081858, Length=341, Percent_Identity=33.7243401759531, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=36.5145228215768, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 75372; Mature: 75241

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFDEAMLAPPEDNMMAETISGLLQRLTPQSGKESLLQAFMPLLPPFSAVQLIELHFPG
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
SRVWYRCFEEGISETAAGGYQGTVVDCSLHAVLLSDMLMVEIRFIRTKGSKFSVQDSSLF
HHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCCEEECCHHHHH
NWLVRIMTPVLESLLGQAEQQATLHTLIKERDHHRVLVDITNAVLTHRDRDELIADVTRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHH
IHHFFGITSVGMVLHDNRPSAGFVLDITHFSPTPAERLQRPLAVESALMQRISGSNQHAL
HHHHHHHHHHCEEEECCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHCCCCCEEE
LHQAEDPLLWQHDPLLQDLHSSGLSCALLLPLTFGRHTSGLLLLAHSSSCLFSDENCTLL
EECCCCCCCCCCCHHHHHHHCCCCCEEEEEEHHCCCCCCCEEEEEECCCCEECCCCCHHH
QHIANRIAIAVDNADAWHNMNDLKEQLKQENNQLNEQLHCSQHVGDIIYQSQVMEELLQQ
HHHHHHEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIVAQSDSTVLLCGETGTGKEVIARTIHQLSLRHDKPLVKINCAAIPAALLESELFGHD
CCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCC
KGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEKEIERLGGNRTIPVN
CCCEEECCCCCCCEEEECCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKYFTQKMARSM
EEEEEECCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KRKIDAISTDTLRQLMSWEWPGNVRELENVIERAVLLTRGGSLNLHLHPRQTRLLPAMSE
HHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCC
NSFTQGQMAQMFNPATPENDEEERLRIIQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQ
CCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHCHHHHHHHHHHH
RLGIAVREVL
HHHHHHHHHC
>Mature Secondary Structure 
TKFDEAMLAPPEDNMMAETISGLLQRLTPQSGKESLLQAFMPLLPPFSAVQLIELHFPG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
SRVWYRCFEEGISETAAGGYQGTVVDCSLHAVLLSDMLMVEIRFIRTKGSKFSVQDSSLF
HHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCCEEECCHHHHH
NWLVRIMTPVLESLLGQAEQQATLHTLIKERDHHRVLVDITNAVLTHRDRDELIADVTRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHH
IHHFFGITSVGMVLHDNRPSAGFVLDITHFSPTPAERLQRPLAVESALMQRISGSNQHAL
HHHHHHHHHHCEEEECCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHCCCCCEEE
LHQAEDPLLWQHDPLLQDLHSSGLSCALLLPLTFGRHTSGLLLLAHSSSCLFSDENCTLL
EECCCCCCCCCCCHHHHHHHCCCCCEEEEEEHHCCCCCCCEEEEEECCCCEECCCCCHHH
QHIANRIAIAVDNADAWHNMNDLKEQLKQENNQLNEQLHCSQHVGDIIYQSQVMEELLQQ
HHHHHHEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGIVAQSDSTVLLCGETGTGKEVIARTIHQLSLRHDKPLVKINCAAIPAALLESELFGHD
CCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCC
KGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEKEIERLGGNRTIPVN
CCCEEECCCCCCCEEEECCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEE
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKYFTQKMARSM
EEEEEECCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
KRKIDAISTDTLRQLMSWEWPGNVRELENVIERAVLLTRGGSLNLHLHPRQTRLLPAMSE
HHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCC
NSFTQGQMAQMFNPATPENDEEERLRIIQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQ
CCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHCHHHHHHHHHHH
RLGIAVREVL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]