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The map label for this gene is lgrB [H]

Identifier: 218532965

GI number: 218532965

Start: 5447542

End: 5450112

Strand: Reverse

Name: lgrB [H]

Synonym: Mchl_5089

Alternate gene names: 218532965

Gene position: 5450112-5447542 (Counterclockwise)

Preceding gene: 218532966

Following gene: 218532964

Centisome position: 94.33

GC content: 73.43

Gene sequence:

>2571_bases
ATGCGGCATTTCACGCAGGAATCGCCGGCCATGACCGGCGAGGTTCACGGCGGCGACGCCTTCACAGCCCGCTGCTCCCA
CAGCCAGACGCGGTTCTGGCTGCTCGACCAGTTGAGGCCCGGCGATCCGAGCCTGCACGTCGCCGCCCGCTGGCGCATCG
CCGGACGGCTCGACGCCGAGATCCTCACCCGTGCCTTCCGCCTCCTGCTCGACCGGCACGAGGCTTTGCGCACGTCGATC
GTCGGCGAGGGTGGCCGGCCGGTGCAGCGGATCGCCGGAACGGTGCCGCTGGCCTTCTCCGAAATCGACCTCTCCGGCCT
GCCCGGCGACCGCCTCGGCGAGGCGCGCGCGATCGCCGAGTCGGAGGCCGGGAAGCCGTTCGATCTGGCCCGCGCGCCGC
TCGTGCGGGTGACGCTGCTGCGGCTCGGGCGCGGCGATTCCCTGCTGCTCGTCACCGCCCATCACAGCATCTGCGACGGC
TGGTCGATGGTGGTGCTGGCCCGCGAGTTCGTCGCGATTTACAGCGCCCTGATCCGGGGACAGGTACCGGCTCTGGCGCC
TGCGGCCCTGCACTATGCCGACTTCGCGGAATGGGAGATCTCCCCGGCCCTCGCCGAGGCGGCGGAACGCGACCTCGCCT
TCTGGGCGCGCTCGTTGCGCGATCTCATCCCGTTCGAGCTGCCGCCGGACCATCCCCGCCGCCCCGGCCGGGAGCGCCGC
TTCGCCGCCGAGACCCGCCCGCTTTCCGCCGATCTCGGTGCGCGCATGGAAGCGTACGCGCGGGCGCAGGGTGCCACCCC
CTTCGTCCTCGCCTGCACCATCCTGTTCGGGCTCCTGAGCGCGCGCACCGGGCGCGGCGACATCGCCGTCGGCACGCAAG
TGCTGGGGCGCGACGAGATCGAGCTGGAAGGGGTCGTCGGCACCTTCGTGAACACGCTCGTGCTTCGGACTCATGTCGAG
GCGGAGCAGAGCTTCGCCGAGCGGCTGGCGGCGACGCGCCGGACGATCCTCGACGCATGCGATCACGCGCTCGCTCCCTT
CGACCGGGTGGTGCAGGCGCTGGCGCCCTGCCGCGACGGGCTGCGACCGCCGCTGGTGTCGGTCAACGTCCGGATGCGGC
CGACACCCCAGGCGACGCCGTCGGGCGGGTCCTCAGTCGGGGAGCCGGCCCCGATCCGCCTCGTCGATCTCGACTACGCC
CCCACCGGCAGCTTCTTCGACCTCGATCTCGAACTTGCGCCGGGCGAGGCCGGCTGGCGCCTGATCTGCGAGTACGATGC
CGGCCTCTACGCGGCGGAGACCGTCGCCGGCCTGCTCGCCGATTTCGAGCAGGCCGCCCGCGCGGCGGTCGGGGCGCGGC
TGCCCGATCCGGCCCCCCTGGGAAGCGGCGGGGGAGGGATCGCGGGCGCGCTTGAACAGGCATCCCGGGAAGCGGGACAG
CCGGAGGCGGAGGAGACGGCCGCGCGGGCGACGCCGGACGCGGCACCTGCGCCCGAGACGGCTCCCCCGTTGGCGGGCGA
GGCGGCGGGCGAACTTCTGCCGACGATACGCACGATCTGGCGGGAGATGCTCGGCCGCCCCGACCTGTCCGACGACGAGG
ATTTCTTCGCGGCGGGCGGGCACTCGCTGCTGGCCGCCCGCGTCGTCGCGCGGATCGAGGCGGCGACGGGCCGAACCGTT
CCGCTCTGGTCGTTCTTCGAGGCGGGCACGGTCGCGCGGCTCTGCGCCTTCCTCGGCGGCGAGGCGGCGGCGGGCGTGCG
GCACCGCGCGCTTCAGGAGCCGACGGCGCAGGCGCGGCCCGGCTTCACCGCGATCCACCACGCTGCGGCCGATCAGGAAC
TCTACCGGCCCCTATCCGAGGCCCTGGGGGCGGACCATCCCTTCGCGACGCTGCAACTGGAAAGCCGGGTTCCGCCCCAC
GAGGAAACCCTGGAGCGGCTGGCCGGCGCCTATCGCGACGCGATCGAGGCCGCGCAGCCGCAGGGGCCGATCCGCCTCGT
CGGGTTCTGCCGCTCGGGCGTCCTCGCCTTCGAGATCGCCCGGCAGTTCGCGCAGGGCGGCCGGGACGTCGCCCTCGTCG
TCCTGATCGATTGCTGGGAGCCCGGCTATATCAGGCGCTTCAGGCCGCTCGCGCGCATGCGGGTGATGGCGGCCTACCGG
CGCGGCCGGCTCGCCGCGCTCATCCGCCACGTCCGCCGCGACGGCATCGGCTACCTCGGCTCGCGGGCCCATCTCTGGTT
CCGCTCGCATCGCGCCTGGCGCGGGTTGCGGCGTGCCCTGAACCGGGTGGGGCTCGCCACCCCGAGCCCGGAGGACGCGT
TCTGGCAAGTGACCGACGAACTCGACGCGCTCGCCCTGGCCTACGAGCCGCGCCCCCATCCCGGGCCGGTGCTGATCGTC
CGCAGCGAGAGTGTTCCGGTCGGCCCCGCGCTCGACCCGATGCTCGGCTGGCGGGCCCATGCCGAGAACATCGAGAGCGT
GAGCGTTCCGGGCTTCGGCCACGAGGGCGCGTTCTCTCCCGCCGGCTGTCGGGTGATGGCCGCCAAGATCAGCCTGATGG
CGTGCCGGTGA

Upstream 100 bases:

>100_bases
GAACACCGGCCGCCGCGCGCGCGCCGATCCCGTCGATCCGGCGCCCAAGGACTGCCGACGCCGACCGCGCCGCGGTCGGT
CAGGCGTGAGGGCGGGCGGG

Downstream 100 bases:

>100_bases
TCCGATCTGCCGCACTTCGCCGCACACGCAGCGGGCGAGCCTAGATCCGCTTGCAATCCATTTTCGGCCGAGAGTAATTT
AGGTTAATAAGCAGCGGGCG

Product: aspartate racemase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent alanine adenylase; AlaA; Alanine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent alanine adenylase; AlaA; Alanine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 856; Mature: 856

Protein sequence:

>856_residues
MRHFTQESPAMTGEVHGGDAFTARCSHSQTRFWLLDQLRPGDPSLHVAARWRIAGRLDAEILTRAFRLLLDRHEALRTSI
VGEGGRPVQRIAGTVPLAFSEIDLSGLPGDRLGEARAIAESEAGKPFDLARAPLVRVTLLRLGRGDSLLLVTAHHSICDG
WSMVVLAREFVAIYSALIRGQVPALAPAALHYADFAEWEISPALAEAAERDLAFWARSLRDLIPFELPPDHPRRPGRERR
FAAETRPLSADLGARMEAYARAQGATPFVLACTILFGLLSARTGRGDIAVGTQVLGRDEIELEGVVGTFVNTLVLRTHVE
AEQSFAERLAATRRTILDACDHALAPFDRVVQALAPCRDGLRPPLVSVNVRMRPTPQATPSGGSSVGEPAPIRLVDLDYA
PTGSFFDLDLELAPGEAGWRLICEYDAGLYAAETVAGLLADFEQAARAAVGARLPDPAPLGSGGGGIAGALEQASREAGQ
PEAEETAARATPDAAPAPETAPPLAGEAAGELLPTIRTIWREMLGRPDLSDDEDFFAAGGHSLLAARVVARIEAATGRTV
PLWSFFEAGTVARLCAFLGGEAAAGVRHRALQEPTAQARPGFTAIHHAAADQELYRPLSEALGADHPFATLQLESRVPPH
EETLERLAGAYRDAIEAAQPQGPIRLVGFCRSGVLAFEIARQFAQGGRDVALVVLIDCWEPGYIRRFRPLARMRVMAAYR
RGRLAALIRHVRRDGIGYLGSRAHLWFRSHRAWRGLRRALNRVGLATPSPEDAFWQVTDELDALALAYEPRPHPGPVLIV
RSESVPVGPALDPMLGWRAHAENIESVSVPGFGHEGAFSPAGCRVMAAKISLMACR

Sequences:

>Translated_856_residues
MRHFTQESPAMTGEVHGGDAFTARCSHSQTRFWLLDQLRPGDPSLHVAARWRIAGRLDAEILTRAFRLLLDRHEALRTSI
VGEGGRPVQRIAGTVPLAFSEIDLSGLPGDRLGEARAIAESEAGKPFDLARAPLVRVTLLRLGRGDSLLLVTAHHSICDG
WSMVVLAREFVAIYSALIRGQVPALAPAALHYADFAEWEISPALAEAAERDLAFWARSLRDLIPFELPPDHPRRPGRERR
FAAETRPLSADLGARMEAYARAQGATPFVLACTILFGLLSARTGRGDIAVGTQVLGRDEIELEGVVGTFVNTLVLRTHVE
AEQSFAERLAATRRTILDACDHALAPFDRVVQALAPCRDGLRPPLVSVNVRMRPTPQATPSGGSSVGEPAPIRLVDLDYA
PTGSFFDLDLELAPGEAGWRLICEYDAGLYAAETVAGLLADFEQAARAAVGARLPDPAPLGSGGGGIAGALEQASREAGQ
PEAEETAARATPDAAPAPETAPPLAGEAAGELLPTIRTIWREMLGRPDLSDDEDFFAAGGHSLLAARVVARIEAATGRTV
PLWSFFEAGTVARLCAFLGGEAAAGVRHRALQEPTAQARPGFTAIHHAAADQELYRPLSEALGADHPFATLQLESRVPPH
EETLERLAGAYRDAIEAAQPQGPIRLVGFCRSGVLAFEIARQFAQGGRDVALVVLIDCWEPGYIRRFRPLARMRVMAAYR
RGRLAALIRHVRRDGIGYLGSRAHLWFRSHRAWRGLRRALNRVGLATPSPEDAFWQVTDELDALALAYEPRPHPGPVLIV
RSESVPVGPALDPMLGWRAHAENIESVSVPGFGHEGAFSPAGCRVMAAKISLMACR
>Mature_856_residues
MRHFTQESPAMTGEVHGGDAFTARCSHSQTRFWLLDQLRPGDPSLHVAARWRIAGRLDAEILTRAFRLLLDRHEALRTSI
VGEGGRPVQRIAGTVPLAFSEIDLSGLPGDRLGEARAIAESEAGKPFDLARAPLVRVTLLRLGRGDSLLLVTAHHSICDG
WSMVVLAREFVAIYSALIRGQVPALAPAALHYADFAEWEISPALAEAAERDLAFWARSLRDLIPFELPPDHPRRPGRERR
FAAETRPLSADLGARMEAYARAQGATPFVLACTILFGLLSARTGRGDIAVGTQVLGRDEIELEGVVGTFVNTLVLRTHVE
AEQSFAERLAATRRTILDACDHALAPFDRVVQALAPCRDGLRPPLVSVNVRMRPTPQATPSGGSSVGEPAPIRLVDLDYA
PTGSFFDLDLELAPGEAGWRLICEYDAGLYAAETVAGLLADFEQAARAAVGARLPDPAPLGSGGGGIAGALEQASREAGQ
PEAEETAARATPDAAPAPETAPPLAGEAAGELLPTIRTIWREMLGRPDLSDDEDFFAAGGHSLLAARVVARIEAATGRTV
PLWSFFEAGTVARLCAFLGGEAAAGVRHRALQEPTAQARPGFTAIHHAAADQELYRPLSEALGADHPFATLQLESRVPPH
EETLERLAGAYRDAIEAAQPQGPIRLVGFCRSGVLAFEIARQFAQGGRDVALVVLIDCWEPGYIRRFRPLARMRVMAAYR
RGRLAALIRHVRRDGIGYLGSRAHLWFRSHRAWRGLRRALNRVGLATPSPEDAFWQVTDELDALALAYEPRPHPGPVLIV
RSESVPVGPALDPMLGWRAHAENIESVSVPGFGHEGAFSPAGCRVMAAKISLMACR

Specific function: Activates the 3rd to 6th amino acids (Ala, D-Leu, Ala and D-Val) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 4th (D-Leu) and the 6th (D-Val) amino acids [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 92346; Mature: 92346

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN ; PS00591 GLYCOSYL_HYDROL_F10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFTQESPAMTGEVHGGDAFTARCSHSQTRFWLLDQLRPGDPSLHVAARWRIAGRLDAE
CCCCCCCCCCCCCEECCCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHH
ILTRAFRLLLDRHEALRTSIVGEGGRPVQRIAGTVPLAFSEIDLSGLPGDRLGEARAIAE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCCCHHCHHHHHHH
SEAGKPFDLARAPLVRVTLLRLGRGDSLLLVTAHHSICDGWSMVVLAREFVAIYSALIRG
HCCCCCHHHHHCHHHHHHHHHCCCCCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHC
QVPALAPAALHYADFAEWEISPALAEAAERDLAFWARSLRDLIPFELPPDHPRRPGRERR
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
FAAETRPLSADLGARMEAYARAQGATPFVLACTILFGLLSARTGRGDIAVGTQVLGRDEI
HHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHCCCCE
ELEGVVGTFVNTLVLRTHVEAEQSFAERLAATRRTILDACDHALAPFDRVVQALAPCRDG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
LRPPLVSVNVRMRPTPQATPSGGSSVGEPAPIRLVDLDYAPTGSFFDLDLELAPGEAGWR
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCE
LICEYDAGLYAAETVAGLLADFEQAARAAVGARLPDPAPLGSGGGGIAGALEQASREAGQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
PEAEETAARATPDAAPAPETAPPLAGEAAGELLPTIRTIWREMLGRPDLSDDEDFFAAGG
CCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
HSLLAARVVARIEAATGRTVPLWSFFEAGTVARLCAFLGGEAAAGVRHRALQEPTAQARP
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCC
GFTAIHHAAADQELYRPLSEALGADHPFATLQLESRVPPHEETLERLAGAYRDAIEAAQP
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
QGPIRLVGFCRSGVLAFEIARQFAQGGRDVALVVLIDCWEPGYIRRFRPLARMRVMAAYR
CCCEEEEEHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
RGRLAALIRHVRRDGIGYLGSRAHLWFRSHRAWRGLRRALNRVGLATPSPEDAFWQVTDE
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHH
LDALALAYEPRPHPGPVLIVRSESVPVGPALDPMLGWRAHAENIESVSVPGFGHEGAFSP
HHHEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHCCHHHHCCCCEECCCCCCCCCCCCC
AGCRVMAAKISLMACR
CCCEEEEEHHHHHCCC
>Mature Secondary Structure
MRHFTQESPAMTGEVHGGDAFTARCSHSQTRFWLLDQLRPGDPSLHVAARWRIAGRLDAE
CCCCCCCCCCCCCEECCCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHH
ILTRAFRLLLDRHEALRTSIVGEGGRPVQRIAGTVPLAFSEIDLSGLPGDRLGEARAIAE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCCCHHCHHHHHHH
SEAGKPFDLARAPLVRVTLLRLGRGDSLLLVTAHHSICDGWSMVVLAREFVAIYSALIRG
HCCCCCHHHHHCHHHHHHHHHCCCCCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHC
QVPALAPAALHYADFAEWEISPALAEAAERDLAFWARSLRDLIPFELPPDHPRRPGRERR
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
FAAETRPLSADLGARMEAYARAQGATPFVLACTILFGLLSARTGRGDIAVGTQVLGRDEI
HHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHCCCCE
ELEGVVGTFVNTLVLRTHVEAEQSFAERLAATRRTILDACDHALAPFDRVVQALAPCRDG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
LRPPLVSVNVRMRPTPQATPSGGSSVGEPAPIRLVDLDYAPTGSFFDLDLELAPGEAGWR
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCE
LICEYDAGLYAAETVAGLLADFEQAARAAVGARLPDPAPLGSGGGGIAGALEQASREAGQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
PEAEETAARATPDAAPAPETAPPLAGEAAGELLPTIRTIWREMLGRPDLSDDEDFFAAGG
CCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
HSLLAARVVARIEAATGRTVPLWSFFEAGTVARLCAFLGGEAAAGVRHRALQEPTAQARP
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCC
GFTAIHHAAADQELYRPLSEALGADHPFATLQLESRVPPHEETLERLAGAYRDAIEAAQP
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
QGPIRLVGFCRSGVLAFEIARQFAQGGRDVALVVLIDCWEPGYIRRFRPLARMRVMAAYR
CCCEEEEEHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
RGRLAALIRHVRRDGIGYLGSRAHLWFRSHRAWRGLRRALNRVGLATPSPEDAFWQVTDE
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHHHH
LDALALAYEPRPHPGPVLIVRSESVPVGPALDPMLGWRAHAENIESVSVPGFGHEGAFSP
HHHEEEEECCCCCCCCEEEEECCCCCCCCCCCHHHCCHHHHCCCCEECCCCCCCCCCCCC
AGCRVMAAKISLMACR
CCCEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA