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The map label for this gene is phaF [H]

Identifier: 218532953

GI number: 218532953

Start: 5434944

End: 5435228

Strand: Reverse

Name: phaF [H]

Synonym: Mchl_5077

Alternate gene names: 218532953

Gene position: 5435228-5434944 (Counterclockwise)

Preceding gene: 218532954

Following gene: 218532952

Centisome position: 94.07

GC content: 68.77

Gene sequence:

>285_bases
GTGAGCGCCGCCACCGTCCTCGATTGGGGCCTCGGCCTCGCCCAAGGCATGCTGGTGCTGGCGATGGCGCTGGCCGCGTG
GCGGATGCTGCGCGGCCCCCGCGCCCAGGACCGTATCCTCGGCCTCGACACGCTCTACCTCAACGGCATGCTGGCGATCG
TCGTCCACGGCATGCGCACCGGCAGCGGCCTCTATTTCGAGGGCGCGCTGATCCTCGGCATGATCGGCTTCACCGCCACC
GTGGCGCTCGCTAAGTTCCTGATGCAAGGCGAGGTGATCCAGTGA

Upstream 100 bases:

>100_bases
CCGGGCGCCTGCTGATGCACCTTCTCGACGACACCGACAGCGAGGGCTGGGTCCGCCGCGTCAAGGACCGCTACGAGCGC
CCCCTGATGGAGATCTTCGC

Downstream 100 bases:

>100_bases
CCGGCGCCGACCTGCCCGCCTGGGCGGCCTGGATCGTCGTGGCGCTGGCGCTCGGTGGCGCCGGCTTCTCGCTGACGGGC
GCGCTCGGGCTCATCCGCCT

Product: multiple resistance and pH regulation protein F

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein F; Pha system subunit F [H]

Number of amino acids: Translated: 94; Mature: 93

Protein sequence:

>94_residues
MSAATVLDWGLGLAQGMLVLAMALAAWRMLRGPRAQDRILGLDTLYLNGMLAIVVHGMRTGSGLYFEGALILGMIGFTAT
VALAKFLMQGEVIQ

Sequences:

>Translated_94_residues
MSAATVLDWGLGLAQGMLVLAMALAAWRMLRGPRAQDRILGLDTLYLNGMLAIVVHGMRTGSGLYFEGALILGMIGFTAT
VALAKFLMQGEVIQ
>Mature_93_residues
SAATVLDWGLGLAQGMLVLAMALAAWRMLRGPRAQDRILGLDTLYLNGMLAIVVHGMRTGSGLYFEGALILGMIGFTATV
ALAKFLMQGEVIQ

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG2212

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhF subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007208 [H]

Pfam domain/function: PF04066 MrpF_PhaF [H]

EC number: NA

Molecular weight: Translated: 10011; Mature: 9880

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
8.5 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATVLDWGLGLAQGMLVLAMALAAWRMLRGPRAQDRILGLDTLYLNGMLAIVVHGMRT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GSGLYFEGALILGMIGFTATVALAKFLMQGEVIQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAATVLDWGLGLAQGMLVLAMALAAWRMLRGPRAQDRILGLDTLYLNGMLAIVVHGMRT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GSGLYFEGALILGMIGFTATVALAKFLMQGEVIQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]