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The map label for this gene is thiE [H]

Identifier: 218532812

GI number: 218532812

Start: 5269479

End: 5270204

Strand: Reverse

Name: thiE [H]

Synonym: Mchl_4935

Alternate gene names: 218532812

Gene position: 5270204-5269479 (Counterclockwise)

Preceding gene: 218532814

Following gene: 218532811

Centisome position: 91.21

GC content: 73.14

Gene sequence:

>726_bases
ATGGGCAGCGGAACAGGGCTCGGTTTGTCCCGGCCGTCGGCGGGCGTCGCCGTGGACCTGCGCCTCTACGGCATCCTCGA
TGTCGGCGTGCTGGGGGGCGATGCCACCGCGCTTGCCACCCTGGCGGCGGAGGCGGTGGCCGGGGGTGCCACCCTGCTCC
AGTACCGCGACAAGGATCTGACCGACGCCCGCGCGGCCCTCGCACGGATCCGGGCGATCCATGCGGCCCTCGCCGGTCGC
GCGCCGCTCCTCGTCAACGACCGGGTCGATCTCGCGCTGGCTGCCGGCGTCGAGGGCGTGCATCTCGGCCAGTCGGATCT
CCACCCCGAAGACGCGCGCCGCCTGCTCGGCCCGCGCGCCATCATCGGGCTCACCGTGAAGACCGGCGCCCAGGCCGACG
AACTCTACCGGCTGCCCATCGACTACGCCTGCATCGGCGGCGTGTTCGCCACCACCAGCAAGGACAACCCCGACCCGCCG
GTCGGGCTCGACGGGTTGCAGCGGATCGTGTTCCGCGCCCGGCTCGCCCGCGGCCAGTCCCTGCCGCTCGGCGCGATCGC
CGGGATCGACGCCAGCAACACTGCCTCCGTCATCCGGGCCGGTGCCGACGGGGTGGCTCTGATCGGCGCCCTGTTCAAGG
GCGGTGCCACCGAGGCCAAGGCGCGGGATCTGCTTGCACGGGTCGATGAGGCGCTGGGCCAGCGTGGGAGCACGGGCACG
CGTTAA

Upstream 100 bases:

>100_bases
AATCGCCCGAACGCGATTGCCCTTGTTCTTTGTTGGCCTCAGTCGCCGCGGCAGGCCGATCCTGCTAGATCGAAAGGCGA
ATTCATCGAAGGGAGCGGGT

Downstream 100 bases:

>100_bases
GGCCGGAAGAAGCGGACCATCAAAAAAATAGACGAGGGAAGACGCCCATGACCGCCATCGCCCTGACCATCGCCGGCTCG
GATTCGAGCGGGGGCGCCGG

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MGSGTGLGLSRPSAGVAVDLRLYGILDVGVLGGDATALATLAAEAVAGGATLLQYRDKDLTDARAALARIRAIHAALAGR
APLLVNDRVDLALAAGVEGVHLGQSDLHPEDARRLLGPRAIIGLTVKTGAQADELYRLPIDYACIGGVFATTSKDNPDPP
VGLDGLQRIVFRARLARGQSLPLGAIAGIDASNTASVIRAGADGVALIGALFKGGATEAKARDLLARVDEALGQRGSTGT
R

Sequences:

>Translated_241_residues
MGSGTGLGLSRPSAGVAVDLRLYGILDVGVLGGDATALATLAAEAVAGGATLLQYRDKDLTDARAALARIRAIHAALAGR
APLLVNDRVDLALAAGVEGVHLGQSDLHPEDARRLLGPRAIIGLTVKTGAQADELYRLPIDYACIGGVFATTSKDNPDPP
VGLDGLQRIVFRARLARGQSLPLGAIAGIDASNTASVIRAGADGVALIGALFKGGATEAKARDLLARVDEALGQRGSTGT
R
>Mature_240_residues
GSGTGLGLSRPSAGVAVDLRLYGILDVGVLGGDATALATLAAEAVAGGATLLQYRDKDLTDARAALARIRAIHAALAGRA
PLLVNDRVDLALAAGVEGVHLGQSDLHPEDARRLLGPRAIIGLTVKTGAQADELYRLPIDYACIGGVFATTSKDNPDPPV
GLDGLQRIVFRARLARGQSLPLGAIAGIDASNTASVIRAGADGVALIGALFKGGATEAKARDLLARVDEALGQRGSTGTR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=171, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24454; Mature: 24323

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSGTGLGLSRPSAGVAVDLRLYGILDVGVLGGDATALATLAAEAVAGGATLLQYRDKDL
CCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHCCHHEEEECCCCH
TDARAALARIRAIHAALAGRAPLLVNDRVDLALAAGVEGVHLGQSDLHPEDARRLLGPRA
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCCEECCCCCCCHHHHHHHHCCCE
IIGLTVKTGAQADELYRLPIDYACIGGVFATTSKDNPDPPVGLDGLQRIVFRARLARGQS
EEEEEEECCCCHHHEEECCCCHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LPLGAIAGIDASNTASVIRAGADGVALIGALFKGGATEAKARDLLARVDEALGQRGSTGT
CCCHHEECCCCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
R
C
>Mature Secondary Structure 
GSGTGLGLSRPSAGVAVDLRLYGILDVGVLGGDATALATLAAEAVAGGATLLQYRDKDL
CCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHCCHHEEEECCCCH
TDARAALARIRAIHAALAGRAPLLVNDRVDLALAAGVEGVHLGQSDLHPEDARRLLGPRA
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCCEECCCCCCCHHHHHHHHCCCE
IIGLTVKTGAQADELYRLPIDYACIGGVFATTSKDNPDPPVGLDGLQRIVFRARLARGQS
EEEEEEECCCCHHHEEECCCCHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LPLGAIAGIDASNTASVIRAGADGVALIGALFKGGATEAKARDLLARVDEALGQRGSTGT
CCCHHEECCCCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]