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The map label for this gene is thiD [H]

Identifier: 218532811

GI number: 218532811

Start: 5268625

End: 5269431

Strand: Reverse

Name: thiD [H]

Synonym: Mchl_4934

Alternate gene names: 218532811

Gene position: 5269431-5268625 (Counterclockwise)

Preceding gene: 218532812

Following gene: 218532810

Centisome position: 91.2

GC content: 72.74

Gene sequence:

>807_bases
ATGACCGCCATCGCCCTGACCATCGCCGGCTCGGATTCGAGCGGGGGCGCCGGCATCCAGGCCGACCTCAAGACCTTCGC
GGCCCTGCGCGTCTACGGCGCCAGCGTCATCACGGCGCTTACGGCCCAGAACACCCGCGGGGTGCAGGCGATCCACGATG
TGCCCGCCGATTTCGTGACCGCGCAGATCGACTCGGTGTTCTCCGATCTCGCGGTCGGGGCGGTGAAGATCGGCATGCTC
TCGCGGCCCGAGACCATCCGGGCGGTGGCCGAGGGTCTGCGGCGGCACGCGCCCGGTATCCCGGTGGTGCTCGACCCGGT
GATGGTGGCCACCAGCGGCGACCGGCTGATCTCGCAGGAAGCCGTCGAGATCCTGCGGACCGAGTTGATGCCGCTGGCCA
CCATCATCACCCCCAACCTGCCCGAAGCCGCGACCCTGCTCGACGAGGGGGAAGCGGGACACGAGAACGAGGCGGTGGCG
CAGGGGCGGCGGCTGCTGGCGCGCGGCGCGCGGGCGGTGCTGATCAAGGGTGGCCACGGCGAGGGCAGCGAGAGCGCCGA
TCACCTGATCGCCGCGGACGGAACCCTGCGCCGGTTCGCGGCCCCGCGCATCGAGACCCGCAACAGCCACGGCACCGGCT
GCACCCTCTCGGCAGCGGTGGCGGCGGGGCTCGCCCGCGGCCAGGGCCTCGCCGAAGCGGTGGGGGGCGCCAAGACCTAC
GTCACGGGGGCGATCCGCGCGGCGGACACGGTGCCGGTCGGCCATGGCCACGGCCCGCTCCACCACTTTTTCGCGCTCTG
GCGATAG

Upstream 100 bases:

>100_bases
TTGCACGGGTCGATGAGGCGCTGGGCCAGCGTGGGAGCACGGGCACGCGTTAAGGCCGGAAGAAGCGGACCATCAAAAAA
ATAGACGAGGGAAGACGCCC

Downstream 100 bases:

>100_bases
GCGCTGGGCTCGGCGAAATCAGCCCCTTTCCGCCTTTTGCGTTTGACAGACCAAACGCTCGTTCGGATAACGGAACGCCT
TTCGGTGCCCCGCACACGAT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTAIALTIAGSDSSGGAGIQADLKTFAALRVYGASVITALTAQNTRGVQAIHDVPADFVTAQIDSVFSDLAVGAVKIGML
SRPETIRAVAEGLRRHAPGIPVVLDPVMVATSGDRLISQEAVEILRTELMPLATIITPNLPEAATLLDEGEAGHENEAVA
QGRRLLARGARAVLIKGGHGEGSESADHLIAADGTLRRFAAPRIETRNSHGTGCTLSAAVAAGLARGQGLAEAVGGAKTY
VTGAIRAADTVPVGHGHGPLHHFFALWR

Sequences:

>Translated_268_residues
MTAIALTIAGSDSSGGAGIQADLKTFAALRVYGASVITALTAQNTRGVQAIHDVPADFVTAQIDSVFSDLAVGAVKIGML
SRPETIRAVAEGLRRHAPGIPVVLDPVMVATSGDRLISQEAVEILRTELMPLATIITPNLPEAATLLDEGEAGHENEAVA
QGRRLLARGARAVLIKGGHGEGSESADHLIAADGTLRRFAAPRIETRNSHGTGCTLSAAVAAGLARGQGLAEAVGGAKTY
VTGAIRAADTVPVGHGHGPLHHFFALWR
>Mature_267_residues
TAIALTIAGSDSSGGAGIQADLKTFAALRVYGASVITALTAQNTRGVQAIHDVPADFVTAQIDSVFSDLAVGAVKIGMLS
RPETIRAVAEGLRRHAPGIPVVLDPVMVATSGDRLISQEAVEILRTELMPLATIITPNLPEAATLLDEGEAGHENEAVAQ
GRRLLARGARAVLIKGGHGEGSESADHLIAADGTLRRFAAPRIETRNSHGTGCTLSAAVAAGLARGQGLAEAVGGAKTYV
TGAIRAADTVPVGHGHGPLHHFFALWR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=57.4144486692015, Blast_Score=285, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=33.5793357933579, Blast_Score=148, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=35.6617647058824, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=35.6617647058824, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27531; Mature: 27399

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIALTIAGSDSSGGAGIQADLKTFAALRVYGASVITALTAQNTRGVQAIHDVPADFVT
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
AQIDSVFSDLAVGAVKIGMLSRPETIRAVAEGLRRHAPGIPVVLDPVMVATSGDRLISQE
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCCCHHHHHH
AVEILRTELMPLATIITPNLPEAATLLDEGEAGHENEAVAQGRRLLARGARAVLIKGGHG
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
EGSESADHLIAADGTLRRFAAPRIETRNSHGTGCTLSAAVAAGLARGQGLAEAVGGAKTY
CCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCHHH
VTGAIRAADTVPVGHGHGPLHHFFALWR
HHHHHHHCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TAIALTIAGSDSSGGAGIQADLKTFAALRVYGASVITALTAQNTRGVQAIHDVPADFVT
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
AQIDSVFSDLAVGAVKIGMLSRPETIRAVAEGLRRHAPGIPVVLDPVMVATSGDRLISQE
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCEEEECCCCHHHHHH
AVEILRTELMPLATIITPNLPEAATLLDEGEAGHENEAVAQGRRLLARGARAVLIKGGHG
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
EGSESADHLIAADGTLRRFAAPRIETRNSHGTGCTLSAAVAAGLARGQGLAEAVGGAKTY
CCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCHHH
VTGAIRAADTVPVGHGHGPLHHFFALWR
HHHHHHHCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]