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The map label for this gene is tuf1 [H]

Identifier: 218532732

GI number: 218532732

Start: 5165588

End: 5166778

Strand: Reverse

Name: tuf1 [H]

Synonym: Mchl_4855

Alternate gene names: 218532732

Gene position: 5166778-5165588 (Counterclockwise)

Preceding gene: 218532733

Following gene: 218532731

Centisome position: 89.42

GC content: 66.5

Gene sequence:

>1191_bases
ATGGCCAAGGAAAAGTTCTCCCGTACCAAGCCGCACTGCAACATCGGCACGATCGGGCACGTGGATCACGGCAAGACGTC
GCTGACGGCGGCGATCACGAAGGTTCTGGCGGAGTCGGGCGGGGCGACCTTCACGGCCTACGACCAGATCGACAAGGCGC
CGGAAGAGAAGGCGCGCGGCATCACGATCTCGACCGCCCACGTCGAGTACGAGACGACCAACCGCCACTACGCGCACGTC
GACTGCCCCGGCCACGCCGACTACGTGAAGAACATGATCACCGGTGCCGCCCAGATGGACGGCGCGATCCTGGTTGTGTC
CGCCGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCGCCCGTCAGGTCGGCGTGCCGGCGCTGGTGGTGT
TCCTCAACAAGGTCGACATGGTCGATGACGAGGAGCTCCTGGAGCTGGTCGAGCTGGAGGTGCGCGAGCTCCTCTCCAAG
TACGACTTCCCCGGCGACGACATCCCGATCACCAAGGGCTCGGCCCTGATGGCGCTCGAGGACAAGGAGCCCAAGATCGG
CAAGGAAGCCGTTCTGGCGCTGATGGCGACGGTCGACGAGTACATCCCGCAGCCGGAGCGTCCGATCGACATGCCGTTCC
TGATGCCGATCGAGGACGTGTTCTCGATCTCGGGCCGCGGCACGGTGGTGACGGGTCGCGTCGAGCGCGGCATCGTCAAG
GTCGGTGAGTCGGTGGAGATCGTCGGCATCCGTCCGACGACGACGACGACGGTGACCGGCGTCGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCGGGCGACAACGTCGGCGTGCTGCTGCGCGGCACGAAGCGCGAGGACGTGGAGCGCGGCCAGG
TCGTGTGCAAGCCGGGTTCGGTCAAGCCGCACTCGAAGTTCAAGGCCGAGGCCTACATCCTGACGAAGGAAGAGGGCGGC
CGCCACACGCCGTTCTTCACCAACTACCGCCCGCAGTTCTACTTCCGCACGACGGACGTGACCGGCGTGTGCACGCTGCC
CGAGGGCACCGAGATGGTGATGCCGGGCGACAACGTGACCATGGACGTGGTGCTGATCGTGCCGGTGGCCATGGAAGAGA
AGCTGCGCTTCGCCATCCGCGAGGGTGGTCGCACCGTCGGTGCCGGCGTCGTCGCCGCCATCAACGACTGA

Upstream 100 bases:

>100_bases
CCTGCCGGCAAACCTGCCGCTTGATCATCGTCGCACAAGCCTCTAATCGGTCCGCGCCTTTACGCCCGGCTTACGGCCGG
TCCCGCGGTAGGATTGCACG

Downstream 100 bases:

>100_bases
TTTTTTTTCGGAATCGGGCGCACGGGTCTCACTCGTGCGCCCGCATCAATCGGACGAAATAAAAAGGGCCGGCGCTGCGC
GCCGGCCCTTTTTTCGTGCA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFTAYDQIDKAPEEKARGITISTAHVEYETTNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSK
YDFPGDDIPITKGSALMALEDKEPKIGKEAVLALMATVDEYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGESVEIVGIRPTTTTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHSKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVTMDVVLIVPVAMEEKLRFAIREGGRTVGAGVVAAIND

Sequences:

>Translated_396_residues
MAKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFTAYDQIDKAPEEKARGITISTAHVEYETTNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSK
YDFPGDDIPITKGSALMALEDKEPKIGKEAVLALMATVDEYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
VGESVEIVGIRPTTTTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHSKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVTMDVVLIVPVAMEEKLRFAIREGGRTVGAGVVAAIND
>Mature_395_residues
AKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFTAYDQIDKAPEEKARGITISTAHVEYETTNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSKY
DFPGDDIPITKGSALMALEDKEPKIGKEAVLALMATVDEYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVKV
GESVEIVGIRPTTTTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGSVKPHSKFKAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVTMDVVLIVPVAMEEKLRFAIREGGRTVGAGVVAAIND

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=390, Percent_Identity=57.1794871794872, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=30.2752293577982, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=30.2752293577982, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=30.6997742663657, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.6875, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194018522, Length=324, Percent_Identity=29.6296296296296, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=30.3030303030303, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=29.6296296296296, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097354, Length=324, Percent_Identity=29.6296296296296, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=28.3018867924528, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI157426893, Length=287, Percent_Identity=26.4808362369338, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=76.1421319796954, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=76.1421319796954, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=382, Percent_Identity=30.6282722513089, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=32.6086956521739, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=271, Percent_Identity=29.1512915129151, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=395, Percent_Identity=53.9240506329114, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=42.9305912596401, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=464, Percent_Identity=31.8965517241379, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=464, Percent_Identity=31.8965517241379, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=29.438202247191, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=28.246013667426, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=28.0182232346241, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=29.1784702549575, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=28.5234899328859, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.961038961039, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=66.751269035533, Blast_Score=537, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=30.5429864253394, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=30.5429864253394, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=27.6353276353276, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=347, Percent_Identity=28.8184438040346, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=26.7489711934156, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=59.3350383631714, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=59.3350383631714, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.21875, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.0438596491228, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.0438596491228, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.0438596491228, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.0438596491228, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=30.4824561403509, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=30.4824561403509, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=31.1046511627907, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.023102310231, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45550900, Length=341, Percent_Identity=29.9120234604106, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647046, Length=302, Percent_Identity=25.4966887417219, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647048, Length=302, Percent_Identity=25.4966887417219, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43121; Mature: 42990

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETTNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSKYDFPGDDIPITKGSALMALE
EEHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEE
DKEPKIGKEAVLALMATVDEYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECHHHCCCEE
VGESVEIVGIRPTTTTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGS
CCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
VKPHSKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVT
CCCCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MDVVLIVPVAMEEKLRFAIREGGRTVGAGVVAAIND
EEEEEEEECHHHHHHHHHHHHCCCEECCEEEEEECC
>Mature Secondary Structure 
AKEKFSRTKPHCNIGTIGHVDHGKTSLTAAITKVLAESGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETTNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPALVVFLNKVDMVDDEELLELVELEVRELLSKYDFPGDDIPITKGSALMALE
EEHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEE
DKEPKIGKEAVLALMATVDEYIPQPERPIDMPFLMPIEDVFSISGRGTVVTGRVERGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHCCCCCCEEEECHHHCCCEE
VGESVEIVGIRPTTTTTVTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVVCKPGS
CCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
VKPHSKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVCTLPEGTEMVMPGDNVT
CCCCCCCCEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MDVVLIVPVAMEEKLRFAIREGGRTVGAGVVAAIND
EEEEEEEECHHHHHHHHHHHHCCCEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA