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The map label for this gene is pbuO [H]

Identifier: 218235926

GI number: 218235926

Start: 779397

End: 780698

Strand: Reverse

Name: pbuO [H]

Synonym: BCB4264_A0804

Alternate gene names: 218235926

Gene position: 780698-779397 (Counterclockwise)

Preceding gene: 218234688

Following gene: 218233100

Centisome position: 14.41

GC content: 38.33

Gene sequence:

>1302_bases
ATGTTTAACCTTTCAAAACATAAAACTTCTATTAAAACTGAAATTATGGCAGGTATTATTACCTTCTTAACAATGGCATA
TATCATTGTCGTAAACCCTGTTATCCTTGGAGATGCAGGTGTTCCATTTGAACAAGCATTTACAGCAACAATTATTGCTG
CTGTTGTCGGAACATTATTTATGGCAATCTTTACAAACTTACCAATCGCAATCGCGCCAGGTATGGGATTAAATGCTTAC
TTCTCTTACTCTGTTGTAAAAGCGCATGAAGGCATGACTTTCGCAATTGCATTCTCTGCTGTATTCGTAGCAGGTATGAT
TTTAATTTTGCTATCATTTACATCTTTCCGTACAAAATTAATGGAAGCAATTCCTGAAAACCTAAAACATGCAATTACTG
CTGGTATCGGGCTATTTATCGCCTTTATCGGTTTACGTTTAACAGGTATCGTTACAAAAAATGATGCAAACTTAGTTGGA
CTTGGTGATCTTCACTCTGCTCCAGTACTACTAGCATTAGCTGGACTTGGAATTACAATTGTCCTTATGTCTTTAAATGT
AAATGGCGCACTATTTATCGGGATGCTATTAACTGGTATTATCGCTTTCTTCACGGGACAATTATCATTCTCAAACGGTG
TTACATCAATGCCTGGTTTACCAGAAGGAATTATCGTTTCAAATCCAATTACTGCTGTATCTGACGTAATTAACTACGGA
TTATACGGCGTTGTATTCTCATTCTTCCTTGTTACACTATTCGATACAACAGGTACATTACTTGGCGTTGCACAACAAGG
TGGATTTATGAAAGACGGAAAGCTTCCAAAAGCAGGACGAGCTCTTCTATCTGACTCATTCTCAGCAACTATTGGTTCTA
TGTTCGGAACGACACCATCAACAGCTTACATTGAATCATCTGCTGGTGTTGCAGCTGGTGGTCGTACTGGTTTAACAACT
GTTACAGTAGCGGTTCTATTCGCACTAGCAGCATTCTTCGGACCATTAGTAAGTGCCGTTTCTGGTGTATCAGCTATTAC
TGCTCCATCATTAATTATCGTTGGTAGTCTTATGATGGGTTCAGTTCGCCACATCGATTGGGACGCATTTGATGAAGCAT
TCCCAGCATTTTTAGTAATCTTAAGCATGCCACTTACATCAAGTATCGCAACAGGTATCGCACTTGGATTTATTTCATAC
CCACTTATGAAAGTGGCAAAAGGTAAATTCCGTGCTGTTCACCCACTTGTGTATGTATTTGGAATTTTGTTTGCTTATCA
ATTGATATTCTTACCACATTAA

Upstream 100 bases:

>100_bases
TGTTATAAACTTTTTATGCAAAACTATTGCAAAATAGATAAAAAACGAATATTATATATAACTGTTGTGAAAATTATTCG
TAAACAGAAAGGATGTTTAT

Downstream 100 bases:

>100_bases
AATCCCTCATAAAGAGGGATTTTTTTATACATAAAAAGTAGAGCATCGAAATTCGATGCTCTATTTTCCTTTTTTAAAAG
CAATATAAAAAGGCTACAGA

Product: xanthine/uracil permease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MFNLSKHKTSIKTEIMAGIITFLTMAYIIVVNPVILGDAGVPFEQAFTATIIAAVVGTLFMAIFTNLPIAIAPGMGLNAY
FSYSVVKAHEGMTFAIAFSAVFVAGMILILLSFTSFRTKLMEAIPENLKHAITAGIGLFIAFIGLRLTGIVTKNDANLVG
LGDLHSAPVLLALAGLGITIVLMSLNVNGALFIGMLLTGIIAFFTGQLSFSNGVTSMPGLPEGIIVSNPITAVSDVINYG
LYGVVFSFFLVTLFDTTGTLLGVAQQGGFMKDGKLPKAGRALLSDSFSATIGSMFGTTPSTAYIESSAGVAAGGRTGLTT
VTVAVLFALAAFFGPLVSAVSGVSAITAPSLIIVGSLMMGSVRHIDWDAFDEAFPAFLVILSMPLTSSIATGIALGFISY
PLMKVAKGKFRAVHPLVYVFGILFAYQLIFLPH

Sequences:

>Translated_433_residues
MFNLSKHKTSIKTEIMAGIITFLTMAYIIVVNPVILGDAGVPFEQAFTATIIAAVVGTLFMAIFTNLPIAIAPGMGLNAY
FSYSVVKAHEGMTFAIAFSAVFVAGMILILLSFTSFRTKLMEAIPENLKHAITAGIGLFIAFIGLRLTGIVTKNDANLVG
LGDLHSAPVLLALAGLGITIVLMSLNVNGALFIGMLLTGIIAFFTGQLSFSNGVTSMPGLPEGIIVSNPITAVSDVINYG
LYGVVFSFFLVTLFDTTGTLLGVAQQGGFMKDGKLPKAGRALLSDSFSATIGSMFGTTPSTAYIESSAGVAAGGRTGLTT
VTVAVLFALAAFFGPLVSAVSGVSAITAPSLIIVGSLMMGSVRHIDWDAFDEAFPAFLVILSMPLTSSIATGIALGFISY
PLMKVAKGKFRAVHPLVYVFGILFAYQLIFLPH
>Mature_433_residues
MFNLSKHKTSIKTEIMAGIITFLTMAYIIVVNPVILGDAGVPFEQAFTATIIAAVVGTLFMAIFTNLPIAIAPGMGLNAY
FSYSVVKAHEGMTFAIAFSAVFVAGMILILLSFTSFRTKLMEAIPENLKHAITAGIGLFIAFIGLRLTGIVTKNDANLVG
LGDLHSAPVLLALAGLGITIVLMSLNVNGALFIGMLLTGIIAFFTGQLSFSNGVTSMPGLPEGIIVSNPITAVSDVINYG
LYGVVFSFFLVTLFDTTGTLLGVAQQGGFMKDGKLPKAGRALLSDSFSATIGSMFGTTPSTAYIESSAGVAAGGRTGLTT
VTVAVLFALAAFFGPLVSAVSGVSAITAPSLIIVGSLMMGSVRHIDWDAFDEAFPAFLVILSMPLTSSIATGIALGFISY
PLMKVAKGKFRAVHPLVYVFGILFAYQLIFLPH

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=432, Percent_Identity=43.5185185185185, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1790150, Length=432, Percent_Identity=41.8981481481481, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1790499, Length=422, Percent_Identity=35.5450236966825, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI48994909, Length=421, Percent_Identity=33.2541567695962, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45244; Mature: 45244

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLSKHKTSIKTEIMAGIITFLTMAYIIVVNPVILGDAGVPFEQAFTATIIAAVVGTLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
MAIFTNLPIAIAPGMGLNAYFSYSVVKAHEGMTFAIAFSAVFVAGMILILLSFTSFRTKL
HHHHHCCCEEEECCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEAIPENLKHAITAGIGLFIAFIGLRLTGIVTKNDANLVGLGDLHSAPVLLALAGLGITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCHHHHHHHHHHHHHEE
VLMSLNVNGALFIGMLLTGIIAFFTGQLSFSNGVTSMPGLPEGIIVSNPITAVSDVINYG
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHH
LYGVVFSFFLVTLFDTTGTLLGVAQQGGFMKDGKLPKAGRALLSDSFSATIGSMFGTTPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCC
TAYIESSAGVAAGGRTGLTTVTVAVLFALAAFFGPLVSAVSGVSAITAPSLIIVGSLMMG
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHC
SVRHIDWDAFDEAFPAFLVILSMPLTSSIATGIALGFISYPLMKVAKGKFRAVHPLVYVF
CHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GILFAYQLIFLPH
HHHHHHHHHHCCC
>Mature Secondary Structure
MFNLSKHKTSIKTEIMAGIITFLTMAYIIVVNPVILGDAGVPFEQAFTATIIAAVVGTLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
MAIFTNLPIAIAPGMGLNAYFSYSVVKAHEGMTFAIAFSAVFVAGMILILLSFTSFRTKL
HHHHHCCCEEEECCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEAIPENLKHAITAGIGLFIAFIGLRLTGIVTKNDANLVGLGDLHSAPVLLALAGLGITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEECCCCCHHHHHHHHHHHHHEE
VLMSLNVNGALFIGMLLTGIIAFFTGQLSFSNGVTSMPGLPEGIIVSNPITAVSDVINYG
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHH
LYGVVFSFFLVTLFDTTGTLLGVAQQGGFMKDGKLPKAGRALLSDSFSATIGSMFGTTPS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCC
TAYIESSAGVAAGGRTGLTTVTVAVLFALAAFFGPLVSAVSGVSAITAPSLIIVGSLMMG
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHC
SVRHIDWDAFDEAFPAFLVILSMPLTSSIATGIALGFISYPLMKVAKGKFRAVHPLVYVF
CHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GILFAYQLIFLPH
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]