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The map label for this gene is mtlA

Identifier: 21672812

GI number: 21672812

Start: 606458

End: 608407

Strand: Reverse

Name: mtlA

Synonym: BUsg552

Alternate gene names: 21672812

Gene position: 608407-606458 (Counterclockwise)

Preceding gene: 21672813

Following gene: 21672811

Centisome position: 94.85

GC content: 24.87

Gene sequence:

>1950_bases
ATGTTTAGATTAATAACATTAAAAATACAAAATTTTGGTCAATTTTTAAGTAATATGATTATGCCTAATATAAGTATATT
TATTACGTGGGGCATCATGAACTCTTTGTTTCTTTCTTTAGATTGGCAATATAATAAAATATTTGCTCAATTAATATCAC
CTATAATATTTTATCTTTTGCCTATTCTTATTGGATATACTGGAGGAAGTTTAATTGCTGGTCAAAGAGGAGGTTTATTA
GGAAGTATAACTACTATAGGAATGATAACAAGTACAGATATGCCTATGTTTTTAGGAGGAATGGTTGCTGGTCCATTAGG
AGGATGGATAATAAAAGGTTTTGATCAAATAATTAAAAACAGAATTAAAAGTGGTTTTGAAATGTTAGTTAATAATTTTT
CAATTGCAATAATTGGAATATTTTTAACAATTTTTTCATTTTTTTTTATTGGTCCCTTCATTGAGGGGGTATCTAAATTT
TTAGGATGTCTAATAAAAATAATAATAGATTTTAATTTGTTACCCCTTATTGCATTTATTATTGAACCAGCTAAGGTATT
TTTTTTAAATAATGCAATTAATCATGGTATATTTTCTCCATTAGGAATTCAAGAAATATCAGAGAATAAAAGTTCTTTAT
TTTTTTTAATAGAATCAAATCCTGGTCCAGGATTAGGCATATTAATAGCTTGCTTTTTTTTCGGAAAAGAAAAATCGTAT
AAATCTTCAGGAACAGCTGCAATTGTTCAATTTTTAGGAGGAATTCATGAAATTTATTTTAGTTATGTATTGATAAAACC
AATATTAATGATTTCTTTAATTTTAGGAAGTATGACAAGTATTTTTATGCTGGTTTTTTTTAAGGGAGGTTTAATTGCTG
CTGTATCACCAGGTTCTATTTTATCTATTTTGGCGATGACAAAAAAAGGTTTATATTTTGCAAATTTAATTTCTATATTT
TCTTCTTTTTTAATTTCTTTTTTGAGTGCAATGTTGTTTTTCAAAATTAATTTAGGTCAAAAAATAAAAAATGATAAAAG
TTCTATACAATTATTTAAAAATAACTTATTTCCTATAAAGAAAATAGGTGATGAAAAATATGATTTTATTAAAAAAATTA
AAAATTTTCAAAAAATTAAGAACATTATAATTGCTTGTGATGCTGGTATGGGATCTAGTGCTATGGGCGCAAGTATTCTG
CGCAAAAAAATAAAAAGTAATAATTTAACACATATTTATGTATCTAATATTGCCATTAATTTGCTACCTAAAAATGCAGA
TTTAGTTATTACACATGAAAAATTAACTTACCTTGCTAAAAAACGCGCTCCTGATGCTCAACATATATCATTAACAAATT
TTCTTGATAGTAATTTTTATACCAGCTTAGTAAAACAATTAGACTTAAATAAAGATTTTTTTAAAAAAAATAATATTCAG
GATAAAAAAGATCGTATTACTCAAATAAATATTGATTCTCATAAAGAAATTCAATCAAAGTGTTTTTTTCAAATAAGTGA
TAAGAATATTTTTCTTAATCAATATGCTGAAAACAAAAAAGAAGCTATCAAAATTGTTGGTAAACATTTGGTAGCACAAG
GATATGTTAAAAAAGATTATATTGATGCAATGCTTGATCGAGAAAAAATTGCTTCAACTTGGTTAGGCGAATCTGTAGCT
TTACCTCATGGTACTATAAAATCTAAAGATTCTATTTTAAAAACTGGAGTAATTTTTTGCCAATTTCCTAAAGGTGTTCA
TTTTGGAGAAACAATCGATGATATTGCTTATCTTGTTATTGGTATCGCTGCAAAGAATAATGAACATATTATGGTAGTTA
GCAATATTACTAATGCATTAGATGATAAAGATGTTATTAGTAAATTATCAAAAACAAAAAGTGTAAAAGAAGTTTTATCA
TATTTAAATGTAAATGTTAAAAAAAACTAA

Upstream 100 bases:

>100_bases
TATAAAGTATTTTAAAAAATTTTTAACTTAAATAAGCTTTTTTTATCTATAGAATTAAAAATGTTTTTTACTGCTATTTT
TCAAGTTTGAGTGAATATTT

Downstream 100 bases:

>100_bases
TTTAAATTGGAATAATTTTATGAGAGCTTTACATTTTGGAGCAGGAAATATTGGACGAGGTTTTATCGCACGAGTATTAT
TAAAATCTGATTTTAATTTG

Product: PTS system mannitol-specific IIABC component

Products: NA

Alternate protein names: EIICBA-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component; Mannitol-specific phosphotransferase enzyme IIA component; PTS system, mannitol-specific EIIA component

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MFRLITLKIQNFGQFLSNMIMPNISIFITWGIMNSLFLSLDWQYNKIFAQLISPIIFYLLPILIGYTGGSLIAGQRGGLL
GSITTIGMITSTDMPMFLGGMVAGPLGGWIIKGFDQIIKNRIKSGFEMLVNNFSIAIIGIFLTIFSFFFIGPFIEGVSKF
LGCLIKIIIDFNLLPLIAFIIEPAKVFFLNNAINHGIFSPLGIQEISENKSSLFFLIESNPGPGLGILIACFFFGKEKSY
KSSGTAAIVQFLGGIHEIYFSYVLIKPILMISLILGSMTSIFMLVFFKGGLIAAVSPGSILSILAMTKKGLYFANLISIF
SSFLISFLSAMLFFKINLGQKIKNDKSSIQLFKNNLFPIKKIGDEKYDFIKKIKNFQKIKNIIIACDAGMGSSAMGASIL
RKKIKSNNLTHIYVSNIAINLLPKNADLVITHEKLTYLAKKRAPDAQHISLTNFLDSNFYTSLVKQLDLNKDFFKKNNIQ
DKKDRITQINIDSHKEIQSKCFFQISDKNIFLNQYAENKKEAIKIVGKHLVAQGYVKKDYIDAMLDREKIASTWLGESVA
LPHGTIKSKDSILKTGVIFCQFPKGVHFGETIDDIAYLVIGIAAKNNEHIMVVSNITNALDDKDVISKLSKTKSVKEVLS
YLNVNVKKN

Sequences:

>Translated_649_residues
MFRLITLKIQNFGQFLSNMIMPNISIFITWGIMNSLFLSLDWQYNKIFAQLISPIIFYLLPILIGYTGGSLIAGQRGGLL
GSITTIGMITSTDMPMFLGGMVAGPLGGWIIKGFDQIIKNRIKSGFEMLVNNFSIAIIGIFLTIFSFFFIGPFIEGVSKF
LGCLIKIIIDFNLLPLIAFIIEPAKVFFLNNAINHGIFSPLGIQEISENKSSLFFLIESNPGPGLGILIACFFFGKEKSY
KSSGTAAIVQFLGGIHEIYFSYVLIKPILMISLILGSMTSIFMLVFFKGGLIAAVSPGSILSILAMTKKGLYFANLISIF
SSFLISFLSAMLFFKINLGQKIKNDKSSIQLFKNNLFPIKKIGDEKYDFIKKIKNFQKIKNIIIACDAGMGSSAMGASIL
RKKIKSNNLTHIYVSNIAINLLPKNADLVITHEKLTYLAKKRAPDAQHISLTNFLDSNFYTSLVKQLDLNKDFFKKNNIQ
DKKDRITQINIDSHKEIQSKCFFQISDKNIFLNQYAENKKEAIKIVGKHLVAQGYVKKDYIDAMLDREKIASTWLGESVA
LPHGTIKSKDSILKTGVIFCQFPKGVHFGETIDDIAYLVIGIAAKNNEHIMVVSNITNALDDKDVISKLSKTKSVKEVLS
YLNVNVKKN
>Mature_649_residues
MFRLITLKIQNFGQFLSNMIMPNISIFITWGIMNSLFLSLDWQYNKIFAQLISPIIFYLLPILIGYTGGSLIAGQRGGLL
GSITTIGMITSTDMPMFLGGMVAGPLGGWIIKGFDQIIKNRIKSGFEMLVNNFSIAIIGIFLTIFSFFFIGPFIEGVSKF
LGCLIKIIIDFNLLPLIAFIIEPAKVFFLNNAINHGIFSPLGIQEISENKSSLFFLIESNPGPGLGILIACFFFGKEKSY
KSSGTAAIVQFLGGIHEIYFSYVLIKPILMISLILGSMTSIFMLVFFKGGLIAAVSPGSILSILAMTKKGLYFANLISIF
SSFLISFLSAMLFFKINLGQKIKNDKSSIQLFKNNLFPIKKIGDEKYDFIKKIKNFQKIKNIIIACDAGMGSSAMGASIL
RKKIKSNNLTHIYVSNIAINLLPKNADLVITHEKLTYLAKKRAPDAQHISLTNFLDSNFYTSLVKQLDLNKDFFKKNNIQ
DKKDRITQINIDSHKEIQSKCFFQISDKNIFLNQYAENKKEAIKIVGKHLVAQGYVKKDYIDAMLDREKIASTWLGESVA
LPHGTIKSKDSILKTGVIFCQFPKGVHFGETIDDIAYLVIGIAAKNNEHIMVVSNITNALDDKDVISKLSKTKSVKEVLS
YLNVNVKKN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1790027, Length=640, Percent_Identity=52.34375, Blast_Score=673, Evalue=0.0,
Organism=Escherichia coli, GI1789301, Length=460, Percent_Identity=42.6086956521739, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788494, Length=141, Percent_Identity=39.7163120567376, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTM3C_BUCAP (Q8K911)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660879.1
- ProteinModelPortal:   Q8K911
- EnsemblBacteria:   EBBUCT00000000069
- GeneID:   1005638
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg552
- GeneTree:   EBGT00050000008060
- HOGENOM:   HBG296680
- OMA:   GMIMPNI
- ProtClustDB:   CLSK2459983
- BioCyc:   BAPH198804:BUSG552-MONOMER
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- Gene3D:   G3DSA:3.40.930.10

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB; SSF55804 PTrfase/Anion_transptr

EC number: =2.7.1.69

Molecular weight: Translated: 72359; Mature: 72359

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 386-386 ACT_SITE 563-563

Signals:

None

Transmembrane regions:

HASH(0xcaa55f4)-; HASH(0xc7f4d64)-; HASH(0xad178c4)-; HASH(0xc2bf5d0)-; HASH(0xcc56604)-; HASH(0xc57a95c)-; HASH(0xcb4549c)-; HASH(0xc5f4200)-; HASH(0xca5fdec)-; HASH(0xc6055ec)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLITLKIQNFGQFLSNMIMPNISIFITWGIMNSLFLSLDWQYNKIFAQLISPIIFYLL
CEEEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
PILIGYTGGSLIAGQRGGLLGSITTIGMITSTDMPMFLGGMVAGPLGGWIIKGFDQIIKN
HHHHHCCCCCEEECCCCCCEEHHHHHEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
RIKSGFEMLVNNFSIAIIGIFLTIFSFFFIGPFIEGVSKFLGCLIKIIIDFNLLPLIAFI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IEPAKVFFLNNAINHGIFSPLGIQEISENKSSLFFLIESNPGPGLGILIACFFFGKEKSY
HCCHHHEEEHHHHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCC
KSSGTAAIVQFLGGIHEIYFSYVLIKPILMISLILGSMTSIFMLVFFKGGLIAAVSPGSI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
LSILAMTKKGLYFANLISIFSSFLISFLSAMLFFKINLGQKIKNDKSSIQLFKNNLFPIK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHCCCCCHH
KIGDEKYDFIKKIKNFQKIKNIIIACDAGMGSSAMGASILRKKIKSNNLTHIYVSNIAIN
HCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECEEEE
LLPKNADLVITHEKLTYLAKKRAPDAQHISLTNFLDSNFYTSLVKQLDLNKDFFKKNNIQ
EECCCCCEEEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
DKKDRITQINIDSHKEIQSKCFFQISDKNIFLNQYAENKKEAIKIVGKHLVAQGYVKKDY
CCCCCEEEECCCCHHHHHHHHEEEECCCEEEEHHHHCCHHHHHHHHHHHHHHHCCHHHHH
IDAMLDREKIASTWLGESVALPHGTIKSKDSILKTGVIFCQFPKGVHFGETIDDIAYLVI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCEEEEECCCCCCCCCHHHHHHHHHH
GIAAKNNEHIMVVSNITNALDDKDVISKLSKTKSVKEVLSYLNVNVKKN
HEEECCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MFRLITLKIQNFGQFLSNMIMPNISIFITWGIMNSLFLSLDWQYNKIFAQLISPIIFYLL
CEEEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
PILIGYTGGSLIAGQRGGLLGSITTIGMITSTDMPMFLGGMVAGPLGGWIIKGFDQIIKN
HHHHHCCCCCEEECCCCCCEEHHHHHEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
RIKSGFEMLVNNFSIAIIGIFLTIFSFFFIGPFIEGVSKFLGCLIKIIIDFNLLPLIAFI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IEPAKVFFLNNAINHGIFSPLGIQEISENKSSLFFLIESNPGPGLGILIACFFFGKEKSY
HCCHHHEEEHHHHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCC
KSSGTAAIVQFLGGIHEIYFSYVLIKPILMISLILGSMTSIFMLVFFKGGLIAAVSPGSI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
LSILAMTKKGLYFANLISIFSSFLISFLSAMLFFKINLGQKIKNDKSSIQLFKNNLFPIK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHCCCCCHH
KIGDEKYDFIKKIKNFQKIKNIIIACDAGMGSSAMGASILRKKIKSNNLTHIYVSNIAIN
HCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECEEEE
LLPKNADLVITHEKLTYLAKKRAPDAQHISLTNFLDSNFYTSLVKQLDLNKDFFKKNNIQ
EECCCCCEEEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
DKKDRITQINIDSHKEIQSKCFFQISDKNIFLNQYAENKKEAIKIVGKHLVAQGYVKKDY
CCCCCEEEECCCCHHHHHHHHEEEECCCEEEEHHHHCCHHHHHHHHHHHHHHHCCHHHHH
IDAMLDREKIASTWLGESVALPHGTIKSKDSILKTGVIFCQFPKGVHFGETIDDIAYLVI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCEEEEECCCCCCCCCHHHHHHHHHH
GIAAKNNEHIMVVSNITNALDDKDVISKLSKTKSVKEVLSYLNVNVKKN
HEEECCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12089438