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The map label for this gene is rnb

Identifier: 21672536

GI number: 21672536

Start: 292269

End: 294209

Strand: Direct

Name: rnb

Synonym: BUsg256

Alternate gene names: 21672536

Gene position: 292269-294209 (Clockwise)

Preceding gene: 21672535

Following gene: 21672537

Centisome position: 45.56

GC content: 27.87

Gene sequence:

>1941_bases
ATGTTCCAAAACAATCCATTACTTGCACAATTAAAAAAAAATCTACATGCTAAAACACCACGGGTTGAGGGAATAGTAAA
AAGCACTGAAAGAGGATTTGGATTTTTAGAAGTTGATGCTCAAAAAAGTTATTTTATACCTCCTAAAAATATGAAAAATG
TTATGCATGGAGATAAAATATCAGCTGTATTAAAAATAGAAAAAGATCGTGAAATAGCTGAACCTGAAAAATTGATTGAA
CCTTTTTTAAATCGATTTGTAGGAAAAATCGAAAAGAAAGATAACAAACTATTTATTTTTCCTGATTATCCATTTTTAAA
AGATTTTATAGCATGTCGACCTAAAAAAAGTTGTGTCCATGTTTTTCAGACAGGAGACTGGGCTGTAGCAAAATTAATTC
AACACAAACTTAATGGAAACCATATATTTTATGCTGAATTAATTGAAGAAATAGTTAAAGCAAATAATCCATTAATACCA
TGGTGGGTAACTTTATCACGTCATAATCTTGAAAGAAAAGAACCTAAAATAGAAAAAGATGATCTTATTTTAAAAAATAA
TTCTCATAGAGAAGATTTAACACATCTTAATTTTATTACAATAGATAATTTTAATACAAAAGATATTGACGACGCTCTTT
TTATTGAAGAAATTCATAATGGTAATCTTCGTTTAATCGTAGCTATTGCAGATCCTACGTCTTATATAAAGAGTGGTAGT
AAATTAGATATAACTGCTGCGAAAAGAGGATTTACAAATTATTTACCAGGTTTTAATGTTCCTATGTTACCTCGAAGTTT
ATCAGAAGATATATGTTCTTTAAATCCTCATGAACGTCGTCCGGTATTAGCATGTTGTATCACAATTTTAAAAGATGGAA
ACATTTGTAGTAAAATTAATTTTTTTTTAGCATGGATTAAATCAAAATCAAAATTATCTTATGATAATGTATCAGATTGG
ATTGAAAAAAAAAGTTCATGGAAACCAGAAACAAAATCAATTGAAAATCAAATATTACTTTTACATCGTTTGTGCTTATT
ACGTATAAAATGGAGAACGTCAAACGCTGTACTATTTCAAGATAGTTTAGAATATAGATTTCAATTTTCTGAAACTGGAA
TTGTAGAAGATGTTGTTATAGAAAAAAGACGCATTGCACATAAAATTATTGAAGAATCAATGATAATAGCAAACATATCT
GCTGCCAACTTTCTATCAAAAAATCTTGGATTTGGAATATATAATACACATGCTGGTTTTGATCCTATTAATGCTGAAAA
TGTTGTTTCCTTTTTAAACAATTATAATTTAAAATTCACTGTAAAAGAAATTACTACTTTGAAGGGATTTTGCAATCTTA
GGCGTGTCTTAAATATATTATCTGATAATTATATTAATAGTCGTATTCGTCGTTATCAGTCTTTCGGAGATTTTAGCATT
ACTCCGAGTCCTCATTTCGCTTTAGGTCTTGTAGAATATGCTACTTGGACCTCTCCTATTCGAAAATACAGTGATATGAT
CAATCACCGTTTGTTAAAATCTATTATCAATCAAGATACAAATATTACTAAACCTAGTGAAGATATAAAACTAAAAATTA
GTGAACAGAAACGTCGCAATAGAATTGCAGAACGAGATATTTCAGATTGGCTTTATACCTTACTTTTACAAAAAAAAGAA
TTTAAGAATAAAAAATTTAATGCTGAAATTATAGATGTTTCAAGAAGTGGAATAAGAGCTAAATTATTAGAAAATGGTGC
AAATGTATTCATTCCTGCTTTATTTTTACATCCTATTCGAGAAGAACTAGTTTTTAATCAAGAAACAGGAAAAGTCTTTA
TTAAAGGTGTTTTATATTACAAGATATCTGATTTAATAACAGTTGTTTTGTCAGAAATTCGATTGCAAACACGTAGTATT
ATTGCTCGAATTGATCATTAA

Upstream 100 bases:

>100_bases
CATTAAAAATTTTAGTAAAATTTATCTATGATATTTTGAAAATAAGTTATATATTAAAATTATTAATTATTTTCTACTAT
AATAATTAGGTAGTATCATT

Downstream 100 bases:

>100_bases
ATATTATCATTTTTAATGATTTCAATATAATTGTAAAAATTCAATATAAATTAAGAGTATAATATGCATATTTCAATGTT
TGATTTTTCTATATATATAA

Product: exoribonuclease II

Products: NA

Alternate protein names: Exoribonuclease II; RNase II; Ribonuclease II

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MFQNNPLLAQLKKNLHAKTPRVEGIVKSTERGFGFLEVDAQKSYFIPPKNMKNVMHGDKISAVLKIEKDREIAEPEKLIE
PFLNRFVGKIEKKDNKLFIFPDYPFLKDFIACRPKKSCVHVFQTGDWAVAKLIQHKLNGNHIFYAELIEEIVKANNPLIP
WWVTLSRHNLERKEPKIEKDDLILKNNSHREDLTHLNFITIDNFNTKDIDDALFIEEIHNGNLRLIVAIADPTSYIKSGS
KLDITAAKRGFTNYLPGFNVPMLPRSLSEDICSLNPHERRPVLACCITILKDGNICSKINFFLAWIKSKSKLSYDNVSDW
IEKKSSWKPETKSIENQILLLHRLCLLRIKWRTSNAVLFQDSLEYRFQFSETGIVEDVVIEKRRIAHKIIEESMIIANIS
AANFLSKNLGFGIYNTHAGFDPINAENVVSFLNNYNLKFTVKEITTLKGFCNLRRVLNILSDNYINSRIRRYQSFGDFSI
TPSPHFALGLVEYATWTSPIRKYSDMINHRLLKSIINQDTNITKPSEDIKLKISEQKRRNRIAERDISDWLYTLLLQKKE
FKNKKFNAEIIDVSRSGIRAKLLENGANVFIPALFLHPIREELVFNQETGKVFIKGVLYYKISDLITVVLSEIRLQTRSI
IARIDH

Sequences:

>Translated_646_residues
MFQNNPLLAQLKKNLHAKTPRVEGIVKSTERGFGFLEVDAQKSYFIPPKNMKNVMHGDKISAVLKIEKDREIAEPEKLIE
PFLNRFVGKIEKKDNKLFIFPDYPFLKDFIACRPKKSCVHVFQTGDWAVAKLIQHKLNGNHIFYAELIEEIVKANNPLIP
WWVTLSRHNLERKEPKIEKDDLILKNNSHREDLTHLNFITIDNFNTKDIDDALFIEEIHNGNLRLIVAIADPTSYIKSGS
KLDITAAKRGFTNYLPGFNVPMLPRSLSEDICSLNPHERRPVLACCITILKDGNICSKINFFLAWIKSKSKLSYDNVSDW
IEKKSSWKPETKSIENQILLLHRLCLLRIKWRTSNAVLFQDSLEYRFQFSETGIVEDVVIEKRRIAHKIIEESMIIANIS
AANFLSKNLGFGIYNTHAGFDPINAENVVSFLNNYNLKFTVKEITTLKGFCNLRRVLNILSDNYINSRIRRYQSFGDFSI
TPSPHFALGLVEYATWTSPIRKYSDMINHRLLKSIINQDTNITKPSEDIKLKISEQKRRNRIAERDISDWLYTLLLQKKE
FKNKKFNAEIIDVSRSGIRAKLLENGANVFIPALFLHPIREELVFNQETGKVFIKGVLYYKISDLITVVLSEIRLQTRSI
IARIDH
>Mature_646_residues
MFQNNPLLAQLKKNLHAKTPRVEGIVKSTERGFGFLEVDAQKSYFIPPKNMKNVMHGDKISAVLKIEKDREIAEPEKLIE
PFLNRFVGKIEKKDNKLFIFPDYPFLKDFIACRPKKSCVHVFQTGDWAVAKLIQHKLNGNHIFYAELIEEIVKANNPLIP
WWVTLSRHNLERKEPKIEKDDLILKNNSHREDLTHLNFITIDNFNTKDIDDALFIEEIHNGNLRLIVAIADPTSYIKSGS
KLDITAAKRGFTNYLPGFNVPMLPRSLSEDICSLNPHERRPVLACCITILKDGNICSKINFFLAWIKSKSKLSYDNVSDW
IEKKSSWKPETKSIENQILLLHRLCLLRIKWRTSNAVLFQDSLEYRFQFSETGIVEDVVIEKRRIAHKIIEESMIIANIS
AANFLSKNLGFGIYNTHAGFDPINAENVVSFLNNYNLKFTVKEITTLKGFCNLRRVLNILSDNYINSRIRRYQSFGDFSI
TPSPHFALGLVEYATWTSPIRKYSDMINHRLLKSIINQDTNITKPSEDIKLKISEQKRRNRIAERDISDWLYTLLLQKKE
FKNKKFNAEIIDVSRSGIRAKLLENGANVFIPALFLHPIREELVFNQETGKVFIKGVLYYKISDLITVVLSEIRLQTRSI
IARIDH

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3' to 5' direction

COG id: COG4776

COG function: function code K; Exoribonuclease II

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI190014623, Length=389, Percent_Identity=30.5912596401028, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI190014625, Length=389, Percent_Identity=30.5912596401028, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI134288890, Length=357, Percent_Identity=27.4509803921569, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI219521928, Length=447, Percent_Identity=26.6219239373602, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI19115966, Length=447, Percent_Identity=26.6219239373602, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1787542, Length=643, Percent_Identity=55.2099533437014, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI87082383, Length=557, Percent_Identity=27.2890484739677, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI212645896, Length=426, Percent_Identity=27.6995305164319, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553506, Length=397, Percent_Identity=26.448362720403, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324552, Length=439, Percent_Identity=27.3348519362187, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323943, Length=349, Percent_Identity=26.647564469914, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24649634, Length=350, Percent_Identity=31.7142857142857, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922976, Length=527, Percent_Identity=23.719165085389, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24654597, Length=527, Percent_Identity=23.719165085389, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24654592, Length=528, Percent_Identity=23.8636363636364, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNB_BUCAP (Q8K9Q5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660603.1
- ProteinModelPortal:   Q8K9Q5
- SMR:   Q8K9Q5
- EnsemblBacteria:   EBBUCT00000000504
- GeneID:   1005458
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg256
- GeneTree:   EBGT00050000007716
- HOGENOM:   HBG401488
- OMA:   MDDALYI
- ProtClustDB:   PRK05054
- BioCyc:   BAPH198804:BUSG256-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01036
- InterPro:   IPR011129
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011804
- PANTHER:   PTHR23355:SF6
- SMART:   SM00357
- SMART:   SM00955
- SMART:   SM00316
- TIGRFAMs:   TIGR00358
- TIGRFAMs:   TIGR02062

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB

EC number: =3.1.13.1

Molecular weight: Translated: 74776; Mature: 74776

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01175 RIBONUCLEASE_II; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQNNPLLAQLKKNLHAKTPRVEGIVKSTERGFGFLEVDAQKSYFIPPKNMKNVMHGDKI
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCE
SAVLKIEKDREIAEPEKLIEPFLNRFVGKIEKKDNKLFIFPDYPFLKDFIACRPKKSCVH
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHEE
VFQTGDWAVAKLIQHKLNGNHIFYAELIEEIVKANNPLIPWWVTLSRHNLERKEPKIEKD
EEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEEEEECCCCHHCCCCCCCC
DLILKNNSHREDLTHLNFITIDNFNTKDIDDALFIEEIHNGNLRLIVAIADPTSYIKSGS
CEEEECCCCCCHHCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHCCCC
KLDITAAKRGFTNYLPGFNVPMLPRSLSEDICSLNPHERRPVLACCITILKDGNICSKIN
EEEEEHHHCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHH
FFLAWIKSKSKLSYDNVSDWIEKKSSWKPETKSIENQILLLHRLCLLRIKWRTSNAVLFQ
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEE
DSLEYRFQFSETGIVEDVVIEKRRIAHKIIEESMIIANISAANFLSKNLGFGIYNTHAGF
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCCEEECCCCC
DPINAENVVSFLNNYNLKFTVKEITTLKGFCNLRRVLNILSDNYINSRIRRYQSFGDFSI
CCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TPSPHFALGLVEYATWTSPIRKYSDMINHRLLKSIINQDTNITKPSEDIKLKISEQKRRN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHH
RIAERDISDWLYTLLLQKKEFKNKKFNAEIIDVSRSGIRAKLLENGANVFIPALFLHPIR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEEEHHHHHHHHH
EELVFNQETGKVFIKGVLYYKISDLITVVLSEIRLQTRSIIARIDH
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFQNNPLLAQLKKNLHAKTPRVEGIVKSTERGFGFLEVDAQKSYFIPPKNMKNVMHGDKI
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCCE
SAVLKIEKDREIAEPEKLIEPFLNRFVGKIEKKDNKLFIFPDYPFLKDFIACRPKKSCVH
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHEE
VFQTGDWAVAKLIQHKLNGNHIFYAELIEEIVKANNPLIPWWVTLSRHNLERKEPKIEKD
EEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEEEEECCCCHHCCCCCCCC
DLILKNNSHREDLTHLNFITIDNFNTKDIDDALFIEEIHNGNLRLIVAIADPTSYIKSGS
CEEEECCCCCCHHCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHCCCC
KLDITAAKRGFTNYLPGFNVPMLPRSLSEDICSLNPHERRPVLACCITILKDGNICSKIN
EEEEEHHHCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHH
FFLAWIKSKSKLSYDNVSDWIEKKSSWKPETKSIENQILLLHRLCLLRIKWRTSNAVLFQ
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEE
DSLEYRFQFSETGIVEDVVIEKRRIAHKIIEESMIIANISAANFLSKNLGFGIYNTHAGF
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCCEEECCCCC
DPINAENVVSFLNNYNLKFTVKEITTLKGFCNLRRVLNILSDNYINSRIRRYQSFGDFSI
CCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TPSPHFALGLVEYATWTSPIRKYSDMINHRLLKSIINQDTNITKPSEDIKLKISEQKRRN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHH
RIAERDISDWLYTLLLQKKEFKNKKFNAEIIDVSRSGIRAKLLENGANVFIPALFLHPIR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEEEHHHHHHHHH
EELVFNQETGKVFIKGVLYYKISDLITVVLSEIRLQTRSIIARIDH
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438