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The map label for this gene is nuoI

Identifier: 21672444

GI number: 21672444

Start: 173808

End: 174350

Strand: Direct

Name: nuoI

Synonym: BUsg154

Alternate gene names: 21672444

Gene position: 173808-174350 (Clockwise)

Preceding gene: 21672443

Following gene: 21672445

Centisome position: 27.1

GC content: 29.47

Gene sequence:

>543_bases
ATGACTGTAAAAGAAATTATTATTGGATTTTTACTGCAAATAAGAAGTATTTTGATGGTTTTCAAAAACATATTTTCTAA
ATCTGAAACTAAAATGTATCCAGAAGAAAAATTATATTTATCTCCTCGATATCGAGGTCGAGTGATTTTAACACGTAATA
TTGATGGAGGAGAACGTTGTGTCGCTTGTAATTTATGTGCTGTAGTGTGTCCTGTTGATTGTATTTCTCTTCAAAAATCC
GAAAAAAAAAATGGTCGTTGGTATCCAAAATTTTTTAGAATCAATTTTTCTCGTTGTATTTTTTGTGGATTATGCGAAGA
AGCATGTCCTACAGCTGCTATTCAACTTATGCCTGATTTTGAATTATCAGATTTTAACAGACAAAATTTAGTTTATGAAA
AAGAAGATTTATTAATTTCGGGTCCAGGAAAATATCCGGATTATGATTTTTATCGTTTTTCTGGTGTAGTTATCAAAGAT
AAGAAGGTAGATAAGTTAGAAGGTCAATTAAAACCTGTAAATGTGAAAGATTTATTACCATAG

Upstream 100 bases:

>100_bases
AATGTCTTTTGGATGGAAAATTTGTTTACCGTTAACGCTATTGAATTTAATTATAACAGCTTTTTTTATATTAATATAAG
CTTTATTAAGAGATAATTAT

Downstream 100 bases:

>100_bases
AGAGGATTAGTTCAATGGAATTTGTTTTTTACGCATGTTCTTTGATAGCTGTTATTTCTACTTTATTAGTTATTATTCAA
AAAAATGCAGTATATTCTTT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTVKEIIIGFLLQIRSILMVFKNIFSKSETKMYPEEKLYLSPRYRGRVILTRNIDGGERCVACNLCAVVCPVDCISLQKS
EKKNGRWYPKFFRINFSRCIFCGLCEEACPTAAIQLMPDFELSDFNRQNLVYEKEDLLISGPGKYPDYDFYRFSGVVIKD
KKVDKLEGQLKPVNVKDLLP

Sequences:

>Translated_180_residues
MTVKEIIIGFLLQIRSILMVFKNIFSKSETKMYPEEKLYLSPRYRGRVILTRNIDGGERCVACNLCAVVCPVDCISLQKS
EKKNGRWYPKFFRINFSRCIFCGLCEEACPTAAIQLMPDFELSDFNRQNLVYEKEDLLISGPGKYPDYDFYRFSGVVIKD
KKVDKLEGQLKPVNVKDLLP
>Mature_179_residues
TVKEIIIGFLLQIRSILMVFKNIFSKSETKMYPEEKLYLSPRYRGRVILTRNIDGGERCVACNLCAVVCPVDCISLQKSE
KKNGRWYPKFFRINFSRCIFCGLCEEACPTAAIQLMPDFELSDFNRQNLVYEKEDLLISGPGKYPDYDFYRFSGVVIKDK
KVDKLEGQLKPVNVKDLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=127, Percent_Identity=40.1574803149606, Blast_Score=107, Evalue=6e-24,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=68.3333333333333, Blast_Score=274, Evalue=2e-75,
Organism=Escherichia coli, GI1789075, Length=117, Percent_Identity=32.4786324786325, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=132, Percent_Identity=38.6363636363636, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17864306, Length=128, Percent_Identity=40.625, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_BUCAP (Q8K9Y0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660511.1
- ProteinModelPortal:   Q8K9Y0
- SMR:   Q8K9Y0
- EnsemblBacteria:   EBBUCT00000000191
- GeneID:   1005352
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg154
- GeneTree:   EBGT00050000007927
- HOGENOM:   HBG724359
- OMA:   RYWSVAG
- ProtClustDB:   PRK05888
- BioCyc:   BAPH198804:BUSG154-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20815; Mature: 20684

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKEIIIGFLLQIRSILMVFKNIFSKSETKMYPEEKLYLSPRYRGRVILTRNIDGGERC
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCEE
VACNLCAVVCPVDCISLQKSEKKNGRWYPKFFRINFSRCIFCGLCEEACPTAAIQLMPDF
EEECHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEEHHHHEEEHHHHHCCCCEEEEECCCC
ELSDFNRQNLVYEKEDLLISGPGKYPDYDFYRFSGVVIKDKKVDKLEGQLKPVNVKDLLP
CCCCCCCCCCEEECCCEEEECCCCCCCCCEEEEEEEEEECCCCHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
TVKEIIIGFLLQIRSILMVFKNIFSKSETKMYPEEKLYLSPRYRGRVILTRNIDGGERC
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCEE
VACNLCAVVCPVDCISLQKSEKKNGRWYPKFFRINFSRCIFCGLCEEACPTAAIQLMPDF
EEECHHHHHCCHHHHHCCCCCCCCCCCCCEEEEEEHHHHEEEHHHHHCCCCEEEEECCCC
ELSDFNRQNLVYEKEDLLISGPGKYPDYDFYRFSGVVIKDKKVDKLEGQLKPVNVKDLLP
CCCCCCCCCCEEECCCEEEECCCCCCCCCEEEEEEEEEECCCCHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438