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The map label for this gene is nth

Identifier: 21672402

GI number: 21672402

Start: 123387

End: 124016

Strand: Direct

Name: nth

Synonym: BUsg111

Alternate gene names: 21672402

Gene position: 123387-124016 (Clockwise)

Preceding gene: 21672401

Following gene: 21672403

Centisome position: 19.24

GC content: 23.97

Gene sequence:

>630_bases
ATGAATAAAAAAAAACGTTTTGAAATTTTGTCTTTATTTTATAAAAAAAACTCTAATCCTAAGATAGAATTAGTTTTTTC
TTCTGATTTTGAATTACTTTTATCAGTTATTTTATCTGCAAAATCAACTGATGTTATGGTAAATAAAATTACTGGTACTT
TATTTCAAATAGCTAATACTCCTCAAAGCATTTTAAAATTAGGTTTTAATAAATTACGACACTATATTAAAAGTATTGGT
TTATATAATACTAAATCTTTAAATATTATTAATAGTGCATATTTAATTAAAACTAAATATAACAATAAAGTTCCATCAAA
TCGTACTGAATTAGAATCTTTACCTGGAGTTGGCAGAAAAACAGCAAATATTATTTTGAATGTATTGTTTAATAAAAACA
CTATTGCTGTAGACACGCATGTTTTCAGAGTTGCTAATCGCACTGGATTTGCTAAAGGGAAAAATGTAATTGAAGTCGAA
AAAAAAATGATTAAGATAGTTCCGTCTATTTTCAAAAAATATGTTCATTTTTGGTTTGTTTTACATGGTAGATATGTTTG
TACTGCTCGTCAATTGAAATGTAAAACATGTTTCATAGAAAAATTATGCGAATTTGACAAAAAAAAATAA

Upstream 100 bases:

>100_bases
GTATTAGGTTTTGTAATTGCTTTCAAAAATTATTTAGATTTAGGTAAAAAAAATTGTTTAAAATGTTTTCATTCGTGTAA
ACTAAAAAAATAATATTAAT

Downstream 100 bases:

>100_bases
GCACTCTATTTATTTTGGGTACATAAGTGATTATTGTAAAAGTTGTTTTACCTTTACCTATTAGAAAGTATTTTAAATAT
TTTATGCCTGATTCTATGTG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNKKKRFEILSLFYKKNSNPKIELVFSSDFELLLSVILSAKSTDVMVNKITGTLFQIANTPQSILKLGFNKLRHYIKSIG
LYNTKSLNIINSAYLIKTKYNNKVPSNRTELESLPGVGRKTANIILNVLFNKNTIAVDTHVFRVANRTGFAKGKNVIEVE
KKMIKIVPSIFKKYVHFWFVLHGRYVCTARQLKCKTCFIEKLCEFDKKK

Sequences:

>Translated_209_residues
MNKKKRFEILSLFYKKNSNPKIELVFSSDFELLLSVILSAKSTDVMVNKITGTLFQIANTPQSILKLGFNKLRHYIKSIG
LYNTKSLNIINSAYLIKTKYNNKVPSNRTELESLPGVGRKTANIILNVLFNKNTIAVDTHVFRVANRTGFAKGKNVIEVE
KKMIKIVPSIFKKYVHFWFVLHGRYVCTARQLKCKTCFIEKLCEFDKKK
>Mature_209_residues
MNKKKRFEILSLFYKKNSNPKIELVFSSDFELLLSVILSAKSTDVMVNKITGTLFQIANTPQSILKLGFNKLRHYIKSIG
LYNTKSLNIINSAYLIKTKYNNKVPSNRTELESLPGVGRKTANIILNVLFNKNTIAVDTHVFRVANRTGFAKGKNVIEVE
KKMIKIVPSIFKKYVHFWFVLHGRYVCTARQLKCKTCFIEKLCEFDKKK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family

Homologues:

Organism=Homo sapiens, GI4505471, Length=193, Percent_Identity=27.4611398963731, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=54.8076923076923, Blast_Score=252, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=28.6516853932584, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END3_BUCAP (Q8KA16)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660469.1
- ProteinModelPortal:   Q8KA16
- SMR:   Q8KA16
- EnsemblBacteria:   EBBUCT00000000123
- GeneID:   1005928
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg111
- GeneTree:   EBGT00050000007867
- HOGENOM:   HBG464473
- OMA:   FGEPTIA
- ProtClustDB:   CLSK315401
- BioCyc:   BAPH198804:BUSG111-MONOMER
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00278
- TIGRFAMs:   TIGR01083

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: =4.2.99.18

Molecular weight: Translated: 24074; Mature: 24074

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKKRFEILSLFYKKNSNPKIELVFSSDFELLLSVILSAKSTDVMVNKITGTLFQIANT
CCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PQSILKLGFNKLRHYIKSIGLYNTKSLNIINSAYLIKTKYNNKVPSNRTELESLPGVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHCCCCCHH
TANIILNVLFNKNTIAVDTHVFRVANRTGFAKGKNVIEVEKKMIKIVPSIFKKYVHFWFV
HHHHHHHHEECCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LHGRYVCTARQLKCKTCFIEKLCEFDKKK
HHCCEEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKKKRFEILSLFYKKNSNPKIELVFSSDFELLLSVILSAKSTDVMVNKITGTLFQIANT
CCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PQSILKLGFNKLRHYIKSIGLYNTKSLNIINSAYLIKTKYNNKVPSNRTELESLPGVGRK
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHCCCCCHH
TANIILNVLFNKNTIAVDTHVFRVANRTGFAKGKNVIEVEKKMIKIVPSIFKKYVHFWFV
HHHHHHHHEECCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LHGRYVCTARQLKCKTCFIEKLCEFDKKK
HHCCEEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438