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The map label for this gene is fliM

Identifier: 21672365

GI number: 21672365

Start: 83971

End: 84906

Strand: Direct

Name: fliM

Synonym: BUsg073

Alternate gene names: 21672365

Gene position: 83971-84906 (Clockwise)

Preceding gene: 21672364

Following gene: 21672366

Centisome position: 13.09

GC content: 18.48

Gene sequence:

>936_bases
GTGCTTCATACAATGGGCAAAAGTACTAATTTAGATAATGAATATAAAATAATTTACAAAAGAGAAAAAAATTTAGATAG
GTTTTTAGATCAGAATGAAATGAAAAAATTAGAAAAAATAAATAAAAAATTTATACAAAAAATAATAATGATTTTTTCTA
GTTTTATTAAAAGTAATATCGAATTAAATTTTTATACTGTAAAAATAAATAGTTATACAAACGATGATAAAAATTTTAAA
CATTTATATGCTAATTCTCTAAAAATAGAAACCTTAGAAAAAAAATCTTTTATATTTTTTTCTCCTAATTTCTTGTCTGT
TTTTATAGATTTTTTATTTGGGGGTAAAGGTAATTCTATAATAAGAATGGATATAAAGAAAGAAATTACCTATACAGAGC
ATGTAATTAATAAAAAAATAGTAGGATTTATCATAGATGCTTATTGTGAATCTTGTAAAGATTTCTTTTATATAGATATA
AAATTTCTTGATTTTAATATTATTAAATTAAATAAGCATTTTTTTTATACAAATGATCTTTTCATAACTAATTATTTTAG
TTTCAATTTAAACGGCATTAAAATATTTTTAAGTATTTTGATTCCTGTTTCAATCATAAAAGAAAAAAATAAAAAAATAA
TTAATTTTTTAGATAAAAATATTTTTTCAAAAAAAACAATTTTAAAAGAAAATTTTTTTATTGAAAATTTATCTAATATT
GAATTAGATGTTACTGTGGAATTAATTCCATCTTATATTTCTAAACATACATTTGATAATTTACGCGAAGGAGATGTGAT
CACAATTGAAGATCCTGGAAAAGTCACAGTATGTATAGAAAAAAAACCTATATTTTTAGGAAAACATAAGATTTTTGATG
AAAAATCTATCATTTTTTTAGAAAAATTTCTTCATCAAAACTTAGAAAAAAAATAA

Upstream 100 bases:

>100_bases
AATACATACTTAATCTTTTTAAGATAAAAATTTTGATTACATTCTTAATGCTTAACAAATCAGTATTTAAGATACTTTTT
TAATAACTTTTACCTATAAG

Downstream 100 bases:

>100_bases
TGTAAGGAAAATTTTAATGAAAAAAGTAGAAAAAAAAGATAATTTTAAAAAATTTAAAAATGATAATATATATAAAAAAG
ATGACAAAAATATTAATCAA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLHTMGKSTNLDNEYKIIYKREKNLDRFLDQNEMKKLEKINKKFIQKIIMIFSSFIKSNIELNFYTVKINSYTNDDKNFK
HLYANSLKIETLEKKSFIFFSPNFLSVFIDFLFGGKGNSIIRMDIKKEITYTEHVINKKIVGFIIDAYCESCKDFFYIDI
KFLDFNIIKLNKHFFYTNDLFITNYFSFNLNGIKIFLSILIPVSIIKEKNKKIINFLDKNIFSKKTILKENFFIENLSNI
ELDVTVELIPSYISKHTFDNLREGDVITIEDPGKVTVCIEKKPIFLGKHKIFDEKSIIFLEKFLHQNLEKK

Sequences:

>Translated_311_residues
MLHTMGKSTNLDNEYKIIYKREKNLDRFLDQNEMKKLEKINKKFIQKIIMIFSSFIKSNIELNFYTVKINSYTNDDKNFK
HLYANSLKIETLEKKSFIFFSPNFLSVFIDFLFGGKGNSIIRMDIKKEITYTEHVINKKIVGFIIDAYCESCKDFFYIDI
KFLDFNIIKLNKHFFYTNDLFITNYFSFNLNGIKIFLSILIPVSIIKEKNKKIINFLDKNIFSKKTILKENFFIENLSNI
ELDVTVELIPSYISKHTFDNLREGDVITIEDPGKVTVCIEKKPIFLGKHKIFDEKSIIFLEKFLHQNLEKK
>Mature_311_residues
MLHTMGKSTNLDNEYKIIYKREKNLDRFLDQNEMKKLEKINKKFIQKIIMIFSSFIKSNIELNFYTVKINSYTNDDKNFK
HLYANSLKIETLEKKSFIFFSPNFLSVFIDFLFGGKGNSIIRMDIKKEITYTEHVINKKIVGFIIDAYCESCKDFFYIDI
KFLDFNIIKLNKHFFYTNDLFITNYFSFNLNGIKIFLSILIPVSIIKEKNKKIINFLDKNIFSKKTILKENFFIENLSNI
ELDVTVELIPSYISKHTFDNLREGDVITIEDPGKVTVCIEKKPIFLGKHKIFDEKSIIFLEKFLHQNLEKK

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Bacterial flagellum basal body (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family

Homologues:

Organism=Escherichia coli, GI1788255, Length=303, Percent_Identity=19.4719471947195, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLIM_BUCAP (Q8KA39)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660432.1
- ProteinModelPortal:   Q8KA39
- EnsemblBacteria:   EBBUCT00000000547
- GeneID:   1005889
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg073
- GeneTree:   EBGT00050000007990
- OMA:   VEVNIIS
- ProtClustDB:   CLSK315201
- BioCyc:   BAPH198804:BUSG073-MONOMER
- GO:   GO:0006935
- InterPro:   IPR001689
- InterPro:   IPR001543

Pfam domain/function: PF02154 FliM; PF01052 SpoA; SSF101801 SpoA

EC number: NA

Molecular weight: Translated: 36927; Mature: 36927

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTMGKSTNLDNEYKIIYKREKNLDRFLDQNEMKKLEKINKKFIQKIIMIFSSFIKSNI
CCCCCCCCCCCCCCEEEEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELNFYTVKINSYTNDDKNFKHLYANSLKIETLEKKSFIFFSPNFLSVFIDFLFGGKGNSI
EEEEEEEEEECCCCCCCCHHHHHHCCEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCE
IRMDIKKEITYTEHVINKKIVGFIIDAYCESCKDFFYIDIKFLDFNIIKLNKHFFYTNDL
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECEEEEECCE
FITNYFSFNLNGIKIFLSILIPVSIIKEKNKKIINFLDKNIFSKKTILKENFFIENLSNI
EEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCE
ELDVTVELIPSYISKHTFDNLREGDVITIEDPGKVTVCIEKKPIFLGKHKIFDEKSIIFL
EEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCEEEECCCCCCCCHHHHH
EKFLHQNLEKK
HHHHHHHCCCC
>Mature Secondary Structure
MLHTMGKSTNLDNEYKIIYKREKNLDRFLDQNEMKKLEKINKKFIQKIIMIFSSFIKSNI
CCCCCCCCCCCCCCEEEEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ELNFYTVKINSYTNDDKNFKHLYANSLKIETLEKKSFIFFSPNFLSVFIDFLFGGKGNSI
EEEEEEEEEECCCCCCCCHHHHHHCCEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCE
IRMDIKKEITYTEHVINKKIVGFIIDAYCESCKDFFYIDIKFLDFNIIKLNKHFFYTNDL
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEECEEEEECCE
FITNYFSFNLNGIKIFLSILIPVSIIKEKNKKIINFLDKNIFSKKTILKENFFIENLSNI
EEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCE
ELDVTVELIPSYISKHTFDNLREGDVITIEDPGKVTVCIEKKPIFLGKHKIFDEKSIIFL
EEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCEEEECCCCCCCCHHHHH
EKFLHQNLEKK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438