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The map label for this gene is trpS2 [H]

Identifier: 213690986

GI number: 213690986

Start: 76841

End: 77932

Strand: Reverse

Name: trpS2 [H]

Synonym: Blon_0064

Alternate gene names: 213690986

Gene position: 77932-76841 (Counterclockwise)

Preceding gene: 213690991

Following gene: 213690984

Centisome position: 2.75

GC content: 63.92

Gene sequence:

>1092_bases
ATGACCGACGAGCAGCAACTGACAGCGGCAGGCAATGAGATAAGCGCGAGCTTCCTCGCGGCCAAAAAGCGTTCGGACGA
AACGCTCGCCAAGCTGGAGGCCAAGCCGTCGTCCTTCACCATGTTGACCGGCGACCGTCCGACCGGCCGGCTGCACCTCG
GCCACTACTTCGGCTCGATCCGTGAGCGTGTCGCCATGCAGGAGCGCGGCGTCAACACGAACATCATCATCGCCGACTAC
CAGGTGATCACCGACCGTGACACCACCGAGCACATCCAAGACAACGTACTGAACCTGGTGCTCGACTACATGGCCGCCGG
CATCGATCCGAACAAGACCATGATGTTCACCCACTCCGCCGTGCCGGCCGAGAACCAGCTGCTGCTGCCGTTCCTGTCCC
TGGTCACCGAGGCTGAGCTGCACCGCAACCCGACCGTGAAGTCCGAGATGGAGGCGTCCGGCCACGCGCTGACCGGTCTG
CTGCTCACCTACCCGGTGCATCAGGCGTGCGACATCCTCTTCTGCAAGGCCAACGTCGTGCCAATCGGTAAGGACAACCT
GCCGCACGTGGAAATCACGCGCACCATCGCCCGCCGTTTCAACGAGCGTTACGCCAAGAAGAACCCAGTGTTCCCGGAGC
CGGCCGCCATTCTGTCTGAGGCACCGGAGATTCCGGGTCTCGACGGCCGCAAGATGAGCAAGTCGTACGGCAACTCCATC
ATGCTGGGCGCCACCGCCGAGGAAACCGCCAAGCTCATCAAGAAGAGCCCGACCGATTCCGAGCGCCGCATCACCTTCGA
CCCGGTTGCCCGCCCGCAGGTCTCCGCCCTGCTGACCACCGCCGGTCTAGTGACCGGCCGCGACCCGAAGGACATCGCCG
AGGAGATCGGTGATTCCGGCGCCGGCGCACTCAAGGCATACGTCATCGAATCAGTCAACGAGTTCCTGGCCCCGCACCGC
GAGCGCCGCGCCGAGCTGGCCAAGGACATGGACTCCATTCGTGACATCCTGCACGACGGCAACAAGCGCGCCAACGCCAT
CGCCGAGGAGACCCTCGACCAGGTGCGCGAGGCCATGGGCATGAAGTACTGA

Upstream 100 bases:

>100_bases
ATCAACAATAGAGAGTACCACTAATAGTGGCTTGTTCGTTGTCGGCTCACACTTTTACCATAAAAAGCGTTTATCTGAGC
CCTATTTAAAGGATTTTCGC

Downstream 100 bases:

>100_bases
TTTTCCCGCCGCTCGTAGCAAGACGCCGTGTCAGCCCCAACTGGGCAACTGACACGGCGTTTTTTGCATTGCGGCGTCTG
CTGTGTCCTATCTTCACTTC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase II; TrpRS II; Tryptophanyl-tRNA synthetase II [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTDEQQLTAAGNEISASFLAAKKRSDETLAKLEAKPSSFTMLTGDRPTGRLHLGHYFGSIRERVAMQERGVNTNIIIADY
QVITDRDTTEHIQDNVLNLVLDYMAAGIDPNKTMMFTHSAVPAENQLLLPFLSLVTEAELHRNPTVKSEMEASGHALTGL
LLTYPVHQACDILFCKANVVPIGKDNLPHVEITRTIARRFNERYAKKNPVFPEPAAILSEAPEIPGLDGRKMSKSYGNSI
MLGATAEETAKLIKKSPTDSERRITFDPVARPQVSALLTTAGLVTGRDPKDIAEEIGDSGAGALKAYVIESVNEFLAPHR
ERRAELAKDMDSIRDILHDGNKRANAIAEETLDQVREAMGMKY

Sequences:

>Translated_363_residues
MTDEQQLTAAGNEISASFLAAKKRSDETLAKLEAKPSSFTMLTGDRPTGRLHLGHYFGSIRERVAMQERGVNTNIIIADY
QVITDRDTTEHIQDNVLNLVLDYMAAGIDPNKTMMFTHSAVPAENQLLLPFLSLVTEAELHRNPTVKSEMEASGHALTGL
LLTYPVHQACDILFCKANVVPIGKDNLPHVEITRTIARRFNERYAKKNPVFPEPAAILSEAPEIPGLDGRKMSKSYGNSI
MLGATAEETAKLIKKSPTDSERRITFDPVARPQVSALLTTAGLVTGRDPKDIAEEIGDSGAGALKAYVIESVNEFLAPHR
ERRAELAKDMDSIRDILHDGNKRANAIAEETLDQVREAMGMKY
>Mature_362_residues
TDEQQLTAAGNEISASFLAAKKRSDETLAKLEAKPSSFTMLTGDRPTGRLHLGHYFGSIRERVAMQERGVNTNIIIADYQ
VITDRDTTEHIQDNVLNLVLDYMAAGIDPNKTMMFTHSAVPAENQLLLPFLSLVTEAELHRNPTVKSEMEASGHALTGLL
LTYPVHQACDILFCKANVVPIGKDNLPHVEITRTIARRFNERYAKKNPVFPEPAAILSEAPEIPGLDGRKMSKSYGNSIM
LGATAEETAKLIKKSPTDSERRITFDPVARPQVSALLTTAGLVTGRDPKDIAEEIGDSGAGALKAYVIESVNEFLAPHRE
RRAELAKDMDSIRDILHDGNKRANAIAEETLDQVREAMGMKY

Specific function: Catalyzes the formation of 5'adenyl-Trp and tRNA(Trp) but with 5-fold less activity than trpRS. Increases the solubility of the nitric oxide synthase oxygenase (nos), as well as its affinity for substrate L-arginine and its nitric-oxide synthase activity.

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=350, Percent_Identity=28.8571428571429, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI41352700, Length=203, Percent_Identity=33.0049261083744, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1789786, Length=327, Percent_Identity=32.1100917431193, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71982800, Length=339, Percent_Identity=30.3834808259587, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982793, Length=333, Percent_Identity=30.9309309309309, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320474, Length=372, Percent_Identity=29.0322580645161, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24666151, Length=328, Percent_Identity=30.7926829268293, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 39949; Mature: 39818

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEQQLTAAGNEISASFLAAKKRSDETLAKLEAKPSSFTMLTGDRPTGRLHLGHYFGSI
CCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHH
RERVAMQERGVNTNIIIADYQVITDRDTTEHIQDNVLNLVLDYMAAGIDPNKTMMFTHSA
HHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
VPAENQLLLPFLSLVTEAELHRNPTVKSEMEASGHALTGLLLTYPVHQACDILFCKANVV
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCHHHHHCEEEEECCEE
PIGKDNLPHVEITRTIARRFNERYAKKNPVFPEPAAILSEAPEIPGLDGRKMSKSYGNSI
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCEE
MLGATAEETAKLIKKSPTDSERRITFDPVARPQVSALLTTAGLVTGRDPKDIAEEIGDSG
EEECCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
AGALKAYVIESVNEFLAPHRERRAELAKDMDSIRDILHDGNKRANAIAEETLDQVREAMG
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
MKY
CCC
>Mature Secondary Structure 
TDEQQLTAAGNEISASFLAAKKRSDETLAKLEAKPSSFTMLTGDRPTGRLHLGHYFGSI
CCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHH
RERVAMQERGVNTNIIIADYQVITDRDTTEHIQDNVLNLVLDYMAAGIDPNKTMMFTHSA
HHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
VPAENQLLLPFLSLVTEAELHRNPTVKSEMEASGHALTGLLLTYPVHQACDILFCKANVV
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCHHHHHCEEEEECCEE
PIGKDNLPHVEITRTIARRFNERYAKKNPVFPEPAAILSEAPEIPGLDGRKMSKSYGNSI
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCEE
MLGATAEETAKLIKKSPTDSERRITFDPVARPQVSALLTTAGLVTGRDPKDIAEEIGDSG
EEECCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
AGALKAYVIESVNEFLAPHRERRAELAKDMDSIRDILHDGNKRANAIAEETLDQVREAMG
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
MKY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]