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The map label for this gene is dmlR [H]

Identifier: 21245062

GI number: 21245062

Start: 5143356

End: 5144288

Strand: Direct

Name: dmlR [H]

Synonym: XAC4350

Alternate gene names: 21245062

Gene position: 5143356-5144288 (Clockwise)

Preceding gene: 21245056

Following gene: 21245063

Centisome position: 99.38

GC content: 71.7

Gene sequence:

>933_bases
ATGGTCCGCTTCGACGACCTGGCGCTGTTCGTACGGACCGCGGCGCTGGGCAGTTTTTCCAGCGCCGCGCGCGAGGCCGA
CCTGTTGCCGGGCCAGGCCAGCGCGGCGGTGGCCCGGCTGGAGCGCGCGCTGGATCTGCGCCTGTTCGCCCGGTCCACGC
GCAGCCTGCGGCTGACCGCCGAGGGCGAGCAGTACCTGCCGTACGCGCGCGGTGTGCTGGAGCTGCTGCGCGACGGGCGC
GAGCATGTGCGGGGCCACGACAGTGCGCTGCAGGGCGTGCTGCAGGTGACGGCGCCGTCGGATCTGGGGCGCAACGTGCT
GCTGCCGTGGCTGACCGAATTCCGCGCTGCACATCCGAAGCTGACCCTGCGCCTGCACCTGTCGGATCAGGTGGCCGACC
TGTATCGCGCGCCGGTGGATGTGGCATTTCGGCTCGGCCGTATCGACGATGCCGAACATGTGGCCTTGCCGGTGGCACCG
GACAACCGCCGCGTGCTGGTGGCCGCACCCGTCTACCTGCAGCGCCACGGGCGCCCCGCCACGCTGGATGCGCTGCGCGA
GCACGCCTGCCTGCTGTACGTGCTGGCCGGGCGCGCCTACCACCGTTGGCAATTCGACCGGGAGGGCCGCCGCCACGTGG
TGCCGGTGCGCGGGGCGATGCTGTGCGACGACGCCGATGTGGTGCGGCGCTGGGCGGTCGCCGGCGAAGGGATCGCCTAC
AAGTCGTGGCTGGACGTGTGTGCCGACGTGCAGGCCGGGCGGCTGGAGGTGCTGTTCCCCGAGTGCGGCGATCGGCTGCC
GCTGCAGCTGGTGTGCCCGCACCGCAAGCAGTTTTCGCCGGCGATCCGGCAATTGCATGCGCTGTTTGCACAGCGCTGCC
AGGCGCTGACGGCGTTGTACCCGGTGCAGGCCGGAGGGCTTGGTGTGGGGTGA

Upstream 100 bases:

>100_bases
TCATGGCAGGGCGTCGTGCGGGAGGGAGTTGCATGGTCGGCCACCCGGATTCACGGAAAAACCGGCAAACTGCGCAAGCA
CTTTCACCATTGGAGTGAAG

Downstream 100 bases:

>100_bases
TGCGGTGATCGCAGTGAAGTGATCGGCACGATTGCTTCGATAGACCAAGGTCGTTTCCCGATGCGGTGCGTACGGAGGCA
CGTCATCGGCATGCCACCGC

Product: transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MVRFDDLALFVRTAALGSFSSAAREADLLPGQASAAVARLERALDLRLFARSTRSLRLTAEGEQYLPYARGVLELLRDGR
EHVRGHDSALQGVLQVTAPSDLGRNVLLPWLTEFRAAHPKLTLRLHLSDQVADLYRAPVDVAFRLGRIDDAEHVALPVAP
DNRRVLVAAPVYLQRHGRPATLDALREHACLLYVLAGRAYHRWQFDREGRRHVVPVRGAMLCDDADVVRRWAVAGEGIAY
KSWLDVCADVQAGRLEVLFPECGDRLPLQLVCPHRKQFSPAIRQLHALFAQRCQALTALYPVQAGGLGVG

Sequences:

>Translated_310_residues
MVRFDDLALFVRTAALGSFSSAAREADLLPGQASAAVARLERALDLRLFARSTRSLRLTAEGEQYLPYARGVLELLRDGR
EHVRGHDSALQGVLQVTAPSDLGRNVLLPWLTEFRAAHPKLTLRLHLSDQVADLYRAPVDVAFRLGRIDDAEHVALPVAP
DNRRVLVAAPVYLQRHGRPATLDALREHACLLYVLAGRAYHRWQFDREGRRHVVPVRGAMLCDDADVVRRWAVAGEGIAY
KSWLDVCADVQAGRLEVLFPECGDRLPLQLVCPHRKQFSPAIRQLHALFAQRCQALTALYPVQAGGLGVG
>Mature_310_residues
MVRFDDLALFVRTAALGSFSSAAREADLLPGQASAAVARLERALDLRLFARSTRSLRLTAEGEQYLPYARGVLELLRDGR
EHVRGHDSALQGVLQVTAPSDLGRNVLLPWLTEFRAAHPKLTLRLHLSDQVADLYRAPVDVAFRLGRIDDAEHVALPVAP
DNRRVLVAAPVYLQRHGRPATLDALREHACLLYVLAGRAYHRWQFDREGRRHVVPVRGAMLCDDADVVRRWAVAGEGIAY
KSWLDVCADVQAGRLEVLFPECGDRLPLQLVCPHRKQFSPAIRQLHALFAQRCQALTALYPVQAGGLGVG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=258, Percent_Identity=29.4573643410853, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786401, Length=267, Percent_Identity=29.5880149812734, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789440, Length=256, Percent_Identity=26.953125, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=25.8389261744966, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=27.1477663230241, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789639, Length=266, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI145693193, Length=282, Percent_Identity=30.1418439716312, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786448, Length=239, Percent_Identity=28.8702928870293, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788706, Length=178, Percent_Identity=29.7752808988764, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34390; Mature: 34390

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRFDDLALFVRTAALGSFSSAAREADLLPGQASAAVARLERALDLRLFARSTRSLRLTA
CCEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
EGEQYLPYARGVLELLRDGREHVRGHDSALQGVLQVTAPSDLGRNVLLPWLTEFRAAHPK
CCCCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCCC
LTLRLHLSDQVADLYRAPVDVAFRLGRIDDAEHVALPVAPDNRRVLVAAPVYLQRHGRPA
EEEEEECCHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCHHHHHCCCCC
TLDALREHACLLYVLAGRAYHRWQFDREGRRHVVPVRGAMLCDDADVVRRWAVAGEGIAY
HHHHHHHHHHHHHHHHCCHHEECCCCCCCCEEEEECCCEEEECCHHHHHHHHHCCCCCCH
KSWLDVCADVQAGRLEVLFPECGDRLPLQLVCPHRKQFSPAIRQLHALFAQRCQALTALY
HHHHHHHHCCCCCCEEEECCCCCCCCCEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHC
PVQAGGLGVG
CCCCCCCCCC
>Mature Secondary Structure
MVRFDDLALFVRTAALGSFSSAAREADLLPGQASAAVARLERALDLRLFARSTRSLRLTA
CCEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEE
EGEQYLPYARGVLELLRDGREHVRGHDSALQGVLQVTAPSDLGRNVLLPWLTEFRAAHPK
CCCCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCCC
LTLRLHLSDQVADLYRAPVDVAFRLGRIDDAEHVALPVAPDNRRVLVAAPVYLQRHGRPA
EEEEEECCHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCHHHHHCCCCC
TLDALREHACLLYVLAGRAYHRWQFDREGRRHVVPVRGAMLCDDADVVRRWAVAGEGIAY
HHHHHHHHHHHHHHHHCCHHEECCCCCCCCEEEEECCCEEEECCHHHHHHHHHCCCCCCH
KSWLDVCADVQAGRLEVLFPECGDRLPLQLVCPHRKQFSPAIRQLHALFAQRCQALTALY
HHHHHHHHCCCCCCEEEECCCCCCCCCEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHC
PVQAGGLGVG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]