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The map label for this gene is vacJ [H]

Identifier: 21245056

GI number: 21245056

Start: 5137518

End: 5138573

Strand: Direct

Name: vacJ [H]

Synonym: XAC4344

Alternate gene names: 21245056

Gene position: 5137518-5138573 (Clockwise)

Preceding gene: 21245055

Following gene: 21245062

Centisome position: 99.27

GC content: 69.32

Gene sequence:

>1056_bases
ATGACCCAGTTCCGTTCTCTTCCGTTGTTGGCCAGCCTGCTGCTGGGTGCCTGCGCCAGCCAGGCGCCCAAATCCGCACC
GCCGTCGGGCGGTACCGCCATCGCGCCGGCAGCCGACGCGCCGGTACGCGACGATGCCGGAGTGACCGCGCAGCCGGCCG
CGTCCGGGATGGCCGCCCCGGATGCGCTGCCGGCGAGCGCTGCTGCTCCTGCCGATCCGCAAGCCGCTGCGGGCGCCGAC
ACCGCCGCTGCAGGCGCGCCCACCGATGCCGAAAGCGACTTCGACGCGCTGTATGGCCCGACCACCTCGCAAGCAAGCGC
CAACGGTACGCCGGCACAACCGGGCGCGGCGCCGGCATACGACCCGTGGGAGCGCTACAACCGCGGCATGCACCGCTTCA
ACATGGCGGTGGACCGCGGCGTGGCGCGTCCGTTGGCGACGGCCTACACCAAGGTGGTGCCCAGCCCGGCGCGCCTGGGC
GTGACCAACTTCTTCGACAACCTGGGCACGCCGCTGACCATGGTCAACCAGCTGCTGCAAGGCCACCCGGTCCATGCGAT
GCAGTCGTTGGGGCGCTTTGTGATGAACAGCACCCTCGGTGTGGCTGGGCTGTTCGACCCCGCTTCGGCGGCCGGTATCC
CGCGGCGCAGCGAAGACTTCGGCCAGACGCTGGGCGCGTGGGGCTGGCGTAACTCGCGCTACTTCGAGCTGCCGTTGTTC
GGGCCGCGCACCGTACGCGACACCTTCGGGCTGGCGGGCGATATTCCGCTGTCGTGGATTCGCCAGGTGGACGACGGCGG
TACGCGCTTCGCCCTGCAGGGCTTGCAGCTGGTGGACACGCGCGCGCAGCTGATGTCGCTGGATTCGCTGCGCGACCAGG
CGCCGGACGAATACGCGCTGACCCGCGATGCGTGGATGCAGCGCCGCAATTACCAGATCACCCGCGATCTGCGCAGCCAT
CGCGAAAAGAACAACGAGCTGCCGGAATACCTGCGCGACGACAAGGACAATACCGTGCCGGGCAACGCCATGCCGATCCC
GAACTGGGTGCGTTGA

Upstream 100 bases:

>100_bases
GGCAGCGGCGAAGTGGTGGGCGAGGCGTCCGGCCTGGACGAATTGCGCGCCGCTTACCGGTTGTCCCCCACTCTCGATTT
TCAGGCCTGAGCGCCAGCCC

Downstream 100 bases:

>100_bases
GCGGTCGGATAGTTGGTCAGTGATGAACGTCGAAGCCCGGGAGCGATCCCGGGCTTTTTTGTGTCAGGCACCAGCCAGAC
CGGTTGCGACGTAACGGCGA

Product: lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTQFRSLPLLASLLLGACASQAPKSAPPSGGTAIAPAADAPVRDDAGVTAQPAASGMAAPDALPASAAAPADPQAAAGAD
TAAAGAPTDAESDFDALYGPTTSQASANGTPAQPGAAPAYDPWERYNRGMHRFNMAVDRGVARPLATAYTKVVPSPARLG
VTNFFDNLGTPLTMVNQLLQGHPVHAMQSLGRFVMNSTLGVAGLFDPASAAGIPRRSEDFGQTLGAWGWRNSRYFELPLF
GPRTVRDTFGLAGDIPLSWIRQVDDGGTRFALQGLQLVDTRAQLMSLDSLRDQAPDEYALTRDAWMQRRNYQITRDLRSH
REKNNELPEYLRDDKDNTVPGNAMPIPNWVR

Sequences:

>Translated_351_residues
MTQFRSLPLLASLLLGACASQAPKSAPPSGGTAIAPAADAPVRDDAGVTAQPAASGMAAPDALPASAAAPADPQAAAGAD
TAAAGAPTDAESDFDALYGPTTSQASANGTPAQPGAAPAYDPWERYNRGMHRFNMAVDRGVARPLATAYTKVVPSPARLG
VTNFFDNLGTPLTMVNQLLQGHPVHAMQSLGRFVMNSTLGVAGLFDPASAAGIPRRSEDFGQTLGAWGWRNSRYFELPLF
GPRTVRDTFGLAGDIPLSWIRQVDDGGTRFALQGLQLVDTRAQLMSLDSLRDQAPDEYALTRDAWMQRRNYQITRDLRSH
REKNNELPEYLRDDKDNTVPGNAMPIPNWVR
>Mature_350_residues
TQFRSLPLLASLLLGACASQAPKSAPPSGGTAIAPAADAPVRDDAGVTAQPAASGMAAPDALPASAAAPADPQAAAGADT
AAAGAPTDAESDFDALYGPTTSQASANGTPAQPGAAPAYDPWERYNRGMHRFNMAVDRGVARPLATAYTKVVPSPARLGV
TNFFDNLGTPLTMVNQLLQGHPVHAMQSLGRFVMNSTLGVAGLFDPASAAGIPRRSEDFGQTLGAWGWRNSRYFELPLFG
PRTVRDTFGLAGDIPLSWIRQVDDGGTRFALQGLQLVDTRAQLMSLDSLRDQAPDEYALTRDAWMQRRNYQITRDLRSHR
EKNNELPEYLRDDKDNTVPGNAMPIPNWVR

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=198, Percent_Identity=40.4040404040404, Blast_Score=124, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 37411; Mature: 37280

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFRSLPLLASLLLGACASQAPKSAPPSGGTAIAPAADAPVRDDAGVTAQPAASGMAAP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
DALPASAAAPADPQAAAGADTAAAGAPTDAESDFDALYGPTTSQASANGTPAQPGAAPAY
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
DPWERYNRGMHRFNMAVDRGVARPLATAYTKVVPSPARLGVTNFFDNLGTPLTMVNQLLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHC
GHPVHAMQSLGRFVMNSTLGVAGLFDPASAAGIPRRSEDFGQTLGAWGWRNSRYFELPLF
CCCHHHHHHHHHHHHHHCCCHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCC
GPRTVRDTFGLAGDIPLSWIRQVDDGGTRFALQGLQLVDTRAQLMSLDSLRDQAPDEYAL
CCCHHHHHHCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TRDAWMQRRNYQITRDLRSHREKNNELPEYLRDDKDNTVPGNAMPIPNWVR
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQFRSLPLLASLLLGACASQAPKSAPPSGGTAIAPAADAPVRDDAGVTAQPAASGMAAP
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
DALPASAAAPADPQAAAGADTAAAGAPTDAESDFDALYGPTTSQASANGTPAQPGAAPAY
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
DPWERYNRGMHRFNMAVDRGVARPLATAYTKVVPSPARLGVTNFFDNLGTPLTMVNQLLQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHC
GHPVHAMQSLGRFVMNSTLGVAGLFDPASAAGIPRRSEDFGQTLGAWGWRNSRYFELPLF
CCCHHHHHHHHHHHHHHCCCHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCC
GPRTVRDTFGLAGDIPLSWIRQVDDGGTRFALQGLQLVDTRAQLMSLDSLRDQAPDEYAL
CCCHHHHHHCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TRDAWMQRRNYQITRDLRSHREKNNELPEYLRDDKDNTVPGNAMPIPNWVR
HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]