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The map label for this gene is yrbE [H]

Identifier: 21245052

GI number: 21245052

Start: 5135262

End: 5136011

Strand: Direct

Name: yrbE [H]

Synonym: XAC4340

Alternate gene names: 21245052

Gene position: 5135262-5136011 (Clockwise)

Preceding gene: 21245051

Following gene: 21245053

Centisome position: 99.22

GC content: 64.93

Gene sequence:

>750_bases
ATGGCCTTCGCCGATTCCATTCGCGCCTTCGGCCGCGCCGGCCTGTTTTCGCTGACGGTGCTGCGCGGCTCGCTGCCCAC
GCGCGATTTCATTGCCGAACTGGTCCGCGAGATCTACAAGGTCGGCGCGCGCTCGTTGCCGATCATTGCGGTGGGCGGCG
CCTTCGTCGGCCTGGTGCTGACCTTGCAGGGCTATCGCACGCTCACCACCTACGGTGCATCGGATGCGTTGTCCACGCTG
TTGGGCCTGTCGCTGTATCGCGAACTGGCGCCGGTGCTGACCGCCTTGTTGTTCATCGGCCGTGCCGGCAGTTCGATCGC
CGCCGAGCTCGGTCTGATGCGCGCCACCGACCAGATCAAGGCGCTGGAACTGATGGCCATCGACCCGGTGGCCAAGGCAG
TGGCCCCGCGCTTCTGGGCGGCGGTGCTGACGGTGCCGCTGCTCACCGGCGTGTTCTGCTCGCTGGCCATCACCGGCGGC
TATTTCGAAGCGGTGCATGTGCTGGGCATCGACAACGGCACGTTCTGGTCGGGTCTGAGCAATAGCGTGGACTTCTGGGA
CGATTTCGGCGTGGCGATGCTCAAGTCGGCGATCTTCGGCGGCACCGCGGCACTGGTTGCCGCCTACGTGGGCTTCCATG
CCGAACCGACCATCGAAGGCACCTCGGTCGCCACCACCCGCGCGGTGGTGAACGCCTCGTTGCTGGTGCTGATGTTCAAC
TTCGTCCTTTCTGCAATGTTGTTTAGGTGA

Upstream 100 bases:

>100_bases
AGGACAGCACCGACCCGCTGGTGCAGCAGTTCCTGCACGGCCAGCCGGACGGCCCGATCGCCTTCGATGCCGCCCCGCGC
CGCGACCGGAGTGCTGCCTG

Downstream 100 bases:

>100_bases
GTGGAATAACTATGGCCATGCGTGGACCACGACTCGAATTCGCCGTCGGCGCCTTTTTGCTGCTGACCCTGGCCTCGCTG
CTGGTGCTGGCGGTGGCCTC

Product: toluene tolerance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MAFADSIRAFGRAGLFSLTVLRGSLPTRDFIAELVREIYKVGARSLPIIAVGGAFVGLVLTLQGYRTLTTYGASDALSTL
LGLSLYRELAPVLTALLFIGRAGSSIAAELGLMRATDQIKALELMAIDPVAKAVAPRFWAAVLTVPLLTGVFCSLAITGG
YFEAVHVLGIDNGTFWSGLSNSVDFWDDFGVAMLKSAIFGGTAALVAAYVGFHAEPTIEGTSVATTRAVVNASLLVLMFN
FVLSAMLFR

Sequences:

>Translated_249_residues
MAFADSIRAFGRAGLFSLTVLRGSLPTRDFIAELVREIYKVGARSLPIIAVGGAFVGLVLTLQGYRTLTTYGASDALSTL
LGLSLYRELAPVLTALLFIGRAGSSIAAELGLMRATDQIKALELMAIDPVAKAVAPRFWAAVLTVPLLTGVFCSLAITGG
YFEAVHVLGIDNGTFWSGLSNSVDFWDDFGVAMLKSAIFGGTAALVAAYVGFHAEPTIEGTSVATTRAVVNASLLVLMFN
FVLSAMLFR
>Mature_248_residues
AFADSIRAFGRAGLFSLTVLRGSLPTRDFIAELVREIYKVGARSLPIIAVGGAFVGLVLTLQGYRTLTTYGASDALSTLL
GLSLYRELAPVLTALLFIGRAGSSIAAELGLMRATDQIKALELMAIDPVAKAVAPRFWAAVLTVPLLTGVFCSLAITGGY
FEAVHVLGIDNGTFWSGLSNSVDFWDDFGVAMLKSAIFGGTAALVAAYVGFHAEPTIEGTSVATTRAVVNASLLVLMFNF
VLSAMLFR

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=244, Percent_Identity=48.3606557377049, Blast_Score=228, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 26280; Mature: 26149

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFADSIRAFGRAGLFSLTVLRGSLPTRDFIAELVREIYKVGARSLPIIAVGGAFVGLVL
CCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHH
TLQGYRTLTTYGASDALSTLLGLSLYRELAPVLTALLFIGRAGSSIAAELGLMRATDQIK
HHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALELMAIDPVAKAVAPRFWAAVLTVPLLTGVFCSLAITGGYFEAVHVLGIDNGTFWSGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCC
NSVDFWDDFGVAMLKSAIFGGTAALVAAYVGFHAEPTIEGTSVATTRAVVNASLLVLMFN
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FVLSAMLFR
HHHHHHHCC
>Mature Secondary Structure 
AFADSIRAFGRAGLFSLTVLRGSLPTRDFIAELVREIYKVGARSLPIIAVGGAFVGLVL
CCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHH
TLQGYRTLTTYGASDALSTLLGLSLYRELAPVLTALLFIGRAGSSIAAELGLMRATDQIK
HHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALELMAIDPVAKAVAPRFWAAVLTVPLLTGVFCSLAITGGYFEAVHVLGIDNGTFWSGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCC
NSVDFWDDFGVAMLKSAIFGGTAALVAAYVGFHAEPTIEGTSVATTRAVVNASLLVLMFN
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FVLSAMLFR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]