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The map label for this gene is acrE [H]

Identifier: 21244713

GI number: 21244713

Start: 4686981

End: 4688108

Strand: Direct

Name: acrE [H]

Synonym: XAC3995

Alternate gene names: 21244713

Gene position: 4686981-4688108 (Clockwise)

Preceding gene: 21244709

Following gene: 21244714

Centisome position: 90.56

GC content: 66.93

Gene sequence:

>1128_bases
TTGGCCGCCGCCGCGTGGTGGTTCACGCGCCGCCCGATGGTGAAGGTGCAGACCGCGCCGGTGGTGGCGATCAGCGCCGG
CAGCAGCAGCGCATCGGTGCTGGACGCCTCCGGCTATGTGGTGGCACGACGCATGGCAACGGTGTCGGCGCAGGTCACCG
GCAAGGTGCGCGAGGTGATGATCGAGGAAGGCATGCGCGTCGAACAAGGCCAGGTGATGGCCACGCTCGACCCGCTCGAT
GCCGATGCGCAGCGTAGTTTGTCGGCCTCGCAGTTGTCGGCCGCACGCAGCCAGGTCGACAACATGCAGGCGCAACTGGC
GGTGGCCAATGCCGATGCCACGCGCCTGCAATCGTTGGTGGGTGCGCAGCTGGTGTCGCGTTCGCAATACGACCAGGCCA
TTGCGCAGCGCGATGCCTTGCGCGCGCAGCTGCAGAACGCCCAGCGCAACGTGCAGGTCGCCTCCGACCAGCTGGCCATT
GCCGGTATCCGCTCGGACTTCAATGTGGTGCGCGCGCCGTTTGCCGGCGTGGTGACGGCCAAAGCAGCGCAGCCTGGCGA
AATCGTCTCGCCGTTGTCGGCCGGTGGCGGCTTCACCCGCACCGGTATCGGCACCATCGTGGACATGGAATCGCTGGAAA
TCGAGGTGGAAGTGGGTGAGTCCTACATCGGCCGCGTGCAACCCAAGATGCCGGTGGAAGCGGTGCTCAATGCCTACCCG
GAGTGGAAGATCCCAGGCGAGGTCATCGCCATCATCCCCACCGCAGACCGCGGCAAGGCCACGGTGAAAGTGCGCGTGGC
GCTCAAGGTCAAGGACCCGCGCATCGTGCCGGAAATGGGTGTGCGGGTCAGCTTTCTGGAGCAGGCCAAACCGCAGCAGG
CAGCCAGGCCGCAAGGCGTGCGCATCCCGGCCACCGCACTGGTGGAGCGCGACACGCGCACGGTCGCGTTCGTGGTCGGC
GAGCACAACACCGTCAGCGCGCATGCCGTCACCACCGGGATGGTGATGGGCGAAGACAGGCAGGTGCTCTCCGGCCTGGC
CGCGGGCGACAGCGTGGTACTGGATCCGCCGCAAGCCTTGAAGGATGGCGACAAGGTGACCGAAGCAGAGCCGAACGCGA
CCGAGTAA

Upstream 100 bases:

>100_bases
TCTGCTCAAGCAACTCCGCATCGACCGCCAGCGCTCCGCCACGCCGCCGCCCGCCCGCCGTACCGGCTGGATCCTCGCCG
CAGTGATCGTCGTCATCGTA

Downstream 100 bases:

>100_bases
CGCACACCCAAAGACAGCGCACGCAGCAACCGCTCAACGGGCACGGAAAAGGGCGTTGATGGAAACGGCAGCGGTGCAGG
CGCCTGATCCGCGCGGCGAC

Product: acriflavin resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAAAAWWFTRRPMVKVQTAPVVAISAGSSSASVLDASGYVVARRMATVSAQVTGKVREVMIEEGMRVEQGQVMATLDPLD
ADAQRSLSASQLSAARSQVDNMQAQLAVANADATRLQSLVGAQLVSRSQYDQAIAQRDALRAQLQNAQRNVQVASDQLAI
AGIRSDFNVVRAPFAGVVTAKAAQPGEIVSPLSAGGGFTRTGIGTIVDMESLEIEVEVGESYIGRVQPKMPVEAVLNAYP
EWKIPGEVIAIIPTADRGKATVKVRVALKVKDPRIVPEMGVRVSFLEQAKPQQAARPQGVRIPATALVERDTRTVAFVVG
EHNTVSAHAVTTGMVMGEDRQVLSGLAAGDSVVLDPPQALKDGDKVTEAEPNATE

Sequences:

>Translated_375_residues
MAAAAWWFTRRPMVKVQTAPVVAISAGSSSASVLDASGYVVARRMATVSAQVTGKVREVMIEEGMRVEQGQVMATLDPLD
ADAQRSLSASQLSAARSQVDNMQAQLAVANADATRLQSLVGAQLVSRSQYDQAIAQRDALRAQLQNAQRNVQVASDQLAI
AGIRSDFNVVRAPFAGVVTAKAAQPGEIVSPLSAGGGFTRTGIGTIVDMESLEIEVEVGESYIGRVQPKMPVEAVLNAYP
EWKIPGEVIAIIPTADRGKATVKVRVALKVKDPRIVPEMGVRVSFLEQAKPQQAARPQGVRIPATALVERDTRTVAFVVG
EHNTVSAHAVTTGMVMGEDRQVLSGLAAGDSVVLDPPQALKDGDKVTEAEPNATE
>Mature_374_residues
AAAAWWFTRRPMVKVQTAPVVAISAGSSSASVLDASGYVVARRMATVSAQVTGKVREVMIEEGMRVEQGQVMATLDPLDA
DAQRSLSASQLSAARSQVDNMQAQLAVANADATRLQSLVGAQLVSRSQYDQAIAQRDALRAQLQNAQRNVQVASDQLAIA
GIRSDFNVVRAPFAGVVTAKAAQPGEIVSPLSAGGGFTRTGIGTIVDMESLEIEVEVGESYIGRVQPKMPVEAVLNAYPE
WKIPGEVIAIIPTADRGKATVKVRVALKVKDPRIVPEMGVRVSFLEQAKPQQAARPQGVRIPATALVERDTRTVAFVVGE
HNTVSAHAVTTGMVMGEDRQVLSGLAAGDSVVLDPPQALKDGDKVTEAEPNATE

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=365, Percent_Identity=27.3972602739726, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081796, Length=351, Percent_Identity=23.9316239316239, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=25.8241758241758, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789665, Length=343, Percent_Identity=24.198250728863, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39667; Mature: 39535

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAAWWFTRRPMVKVQTAPVVAISAGSSSASVLDASGYVVARRMATVSAQVTGKVREVM
CCCCEEEECCCCEEEEECCCEEEEECCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHH
IEEGMRVEQGQVMATLDPLDADAQRSLSASQLSAARSQVDNMQAQLAVANADATRLQSLV
HHCCCEECCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHH
GAQLVSRSQYDQAIAQRDALRAQLQNAQRNVQVASDQLAIAGIRSDFNVVRAPFAGVVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCHHHHCCCCCEEEE
KAAQPGEIVSPLSAGGGFTRTGIGTIVDMESLEIEVEVGESYIGRVQPKMPVEAVLNAYP
CCCCCCHHCCCCCCCCCEEECCCCEEEEEEHEEEEEEECCHHHCCCCCCCCHHHHHCCCC
EWKIPGEVIAIIPTADRGKATVKVRVALKVKDPRIVPEMGVRVSFLEQAKPQQAARPQGV
CCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCHHHCCEEEEHHHCCCHHHCCCCCC
RIPATALVERDTRTVAFVVGEHNTVSAHAVTTGMVMGEDRQVLSGLAAGDSVVLDPPQAL
CCCHHHEEECCCCEEEEEEECCCEEEHHEEEEEEEECCCHHHHHHHCCCCEEEECCHHHH
KDGDKVTEAEPNATE
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAAAWWFTRRPMVKVQTAPVVAISAGSSSASVLDASGYVVARRMATVSAQVTGKVREVM
CCCEEEECCCCEEEEECCCEEEEECCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHH
IEEGMRVEQGQVMATLDPLDADAQRSLSASQLSAARSQVDNMQAQLAVANADATRLQSLV
HHCCCEECCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHH
GAQLVSRSQYDQAIAQRDALRAQLQNAQRNVQVASDQLAIAGIRSDFNVVRAPFAGVVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCHHHHCCCCCEEEE
KAAQPGEIVSPLSAGGGFTRTGIGTIVDMESLEIEVEVGESYIGRVQPKMPVEAVLNAYP
CCCCCCHHCCCCCCCCCEEECCCCEEEEEEHEEEEEEECCHHHCCCCCCCCHHHHHCCCC
EWKIPGEVIAIIPTADRGKATVKVRVALKVKDPRIVPEMGVRVSFLEQAKPQQAARPQGV
CCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCHHHCCEEEEHHHCCCHHHCCCCCC
RIPATALVERDTRTVAFVVGEHNTVSAHAVTTGMVMGEDRQVLSGLAAGDSVVLDPPQAL
CCCHHHEEECCCCEEEEEEECCCEEEHHEEEEEEEECCCHHHHHHHCCCCEEEECCHHHH
KDGDKVTEAEPNATE
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]