BacMap

Definition	Xanthomonas axonopodis pv. citri str. 306 chromosome, complete genome.
Accession	NC_003919
Length	5,175,554

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The map label for this gene is hdhA [C]

Identifier: 21244401

GI number: 21244401

Start: 4353831

End: 4354631

Strand: Direct

Name: hdhA [C]

Synonym: XAC3676

Alternate gene names: 21244401

Gene position: 4353831-4354631 (Clockwise)

Preceding gene: 21244400

Following gene: 21244405

Centisome position: 84.12

GC content: 69.66

Gene sequence:

>801_bases
TTGATCCCCGCAATTTCCGCATGGAACCCGTCGCCGCTGCAGGACCGCGTGGTGGTGATCACCGGTGGGGCGCAAGGCAT
TGGCCGCGGCATTGCACAGGCGGTGCTGGGCGCGGGCGGCAGCGTGGTGATTGGCGATCTGGATGCCGATGCGGGCAAGG
CCTGCCTACAGGAATGGGCACTGCCGCGCCGCAGCGCTTTCGTACGCTGCGATGCCGCGCGCCAGGCGCAGGCCACGCGC
CTGGTCGCCACGGCGCTCAAGCGCTTCGGGCGCATCGACGGCCTGGTCAACAATGCCGGTGTGCCCGATCCGCGTATCGC
AGCGCTGCCGCAACTGGACTGGGACACATGGAACAGCCGGCTTTCCAGCCTGCACGGCGCCTTCCTGTGCAGCAAGCAGG
CACTGCCCGCGCTCAGGCAGGCGCCAGCCGGTGGCGCGATCGTCAACATCGCCTCCACCCGCGCCTGGCAATCGGAGCCG
CACAGCGAAGCCTACGCCGCAGCCAAGGGCGGCCTGGTGGCCTTTACCCACGCGCTGGCGCTGAGCGAGGGGCCGCATGT
GCGCGTCAACAGCATCAGCCCGGGGTGGATCAGCACCGATGCGTGGCGCGCGCCGCAACGCCGGCGGGCGCCGAAATTGT
CGCGTCGCGACCATGCGCAGCATCCGGCCGGGCGCGTGGGCACGCCCGAAGACATTGCGCAGCTGGCGGTGTATCTGCTC
GCGCCGCAGCTGTCCGGCTTCGTCACCGGGCAGGATTTCATCGTCGACGGCGGCATGTCGCGCAAGATGCACTACGCGTA
G

Upstream 100 bases:

>100_bases
ACGTGCGCCGAATTCTCATACGCGCCCCACGTTGCTGCGACGACGACAACCCCCGCGCTACAGTGCCGCCACCCTCACCC
ACGTGCCGCATGCCATGGCC

Downstream 100 bases:

>100_bases
AGCGTGTTATGTGCTTTGAGGTGAGTGCGAAGCGTCCTGTGGCGCGTCCCGGAGGGTTGGCCGTCAGCGGCTCGGAGCGC
GCGCTGCGCGGCTTGCCTTG

Product: oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MIPAISAWNPSPLQDRVVVITGGAQGIGRGIAQAVLGAGGSVVIGDLDADAGKACLQEWALPRRSAFVRCDAARQAQATR
LVATALKRFGRIDGLVNNAGVPDPRIAALPQLDWDTWNSRLSSLHGAFLCSKQALPALRQAPAGGAIVNIASTRAWQSEP
HSEAYAAAKGGLVAFTHALALSEGPHVRVNSISPGWISTDAWRAPQRRRAPKLSRRDHAQHPAGRVGTPEDIAQLAVYLL
APQLSGFVTGQDFIVDGGMSRKMHYA

Sequences:

>Translated_266_residues
MIPAISAWNPSPLQDRVVVITGGAQGIGRGIAQAVLGAGGSVVIGDLDADAGKACLQEWALPRRSAFVRCDAARQAQATR
LVATALKRFGRIDGLVNNAGVPDPRIAALPQLDWDTWNSRLSSLHGAFLCSKQALPALRQAPAGGAIVNIASTRAWQSEP
HSEAYAAAKGGLVAFTHALALSEGPHVRVNSISPGWISTDAWRAPQRRRAPKLSRRDHAQHPAGRVGTPEDIAQLAVYLL
APQLSGFVTGQDFIVDGGMSRKMHYA
>Mature_266_residues
MIPAISAWNPSPLQDRVVVITGGAQGIGRGIAQAVLGAGGSVVIGDLDADAGKACLQEWALPRRSAFVRCDAARQAQATR
LVATALKRFGRIDGLVNNAGVPDPRIAALPQLDWDTWNSRLSSLHGAFLCSKQALPALRQAPAGGAIVNIASTRAWQSEP
HSEAYAAAKGGLVAFTHALALSEGPHVRVNSISPGWISTDAWRAPQRRRAPKLSRRDHAQHPAGRVGTPEDIAQLAVYLL
APQLSGFVTGQDFIVDGGMSRKMHYA

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI59889578, Length=237, Percent_Identity=35.8649789029536, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=31.4393939393939, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI31542939, Length=219, Percent_Identity=32.4200913242009, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI40254992, Length=247, Percent_Identity=30.7692307692308, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI32483357, Length=261, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI15277342, Length=266, Percent_Identity=29.6992481203008, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI5031737, Length=254, Percent_Identity=30.3149606299213, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI224922801, Length=174, Percent_Identity=35.0574712643678, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=31.2741312741313, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4504505, Length=196, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=26.2548262548263, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI33667109, Length=193, Percent_Identity=32.1243523316062, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=28.494623655914, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI193804850, Length=216, Percent_Identity=28.7037037037037, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=31.3725490196078, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=31.6, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=30.241935483871, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=31.6, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=27.6679841897233, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786812, Length=247, Percent_Identity=31.1740890688259, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=30.46875, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=27.8884462151394, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI2367175, Length=249, Percent_Identity=29.3172690763052, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789057, Length=257, Percent_Identity=25.6809338521401, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=264, Percent_Identity=29.9242424242424, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=29.6577946768061, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=30.8270676691729, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562904, Length=267, Percent_Identity=29.9625468164794, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=32.3308270676692, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=31.4393939393939, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17562990, Length=273, Percent_Identity=32.2344322344322, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25147288, Length=252, Percent_Identity=30.5555555555556, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72000259, Length=267, Percent_Identity=31.0861423220974, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560676, Length=250, Percent_Identity=28.4, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562906, Length=261, Percent_Identity=28.735632183908, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=28.4584980237154, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=29.811320754717, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=28.0898876404494, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=266, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=27.8625954198473, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=258, Percent_Identity=30.2325581395349, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17551412, Length=277, Percent_Identity=28.8808664259928, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=28.8973384030418, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17544670, Length=269, Percent_Identity=26.3940520446097, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=28.90625, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=27.0992366412214, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=256, Percent_Identity=26.5625, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=257, Percent_Identity=26.0700389105058, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=193, Percent_Identity=29.0155440414508, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=260, Percent_Identity=25.7692307692308, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=260, Percent_Identity=28.4615384615385, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322861, Length=196, Percent_Identity=31.6326530612245, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=33.201581027668, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=31.7460317460317, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=26.953125, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28571526, Length=259, Percent_Identity=30.5019305019305, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=31.0077519379845, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=29.8804780876494, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24643142, Length=259, Percent_Identity=30.5019305019305, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644337, Length=172, Percent_Identity=34.3023255813954, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571387, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641232, Length=241, Percent_Identity=27.3858921161826, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28136; Mature: 28136

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPAISAWNPSPLQDRVVVITGGAQGIGRGIAQAVLGAGGSVVIGDLDADAGKACLQEWA
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHC
LPRRSAFVRCDAARQAQATRLVATALKRFGRIDGLVNNAGVPDPRIAALPQLDWDTWNSR
CCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHH
LSSLHGAFLCSKQALPALRQAPAGGAIVNIASTRAWQSEPHSEAYAAAKGGLVAFTHALA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
LSEGPHVRVNSISPGWISTDAWRAPQRRRAPKLSRRDHAQHPAGRVGTPEDIAQLAVYLL
HCCCCCEEEECCCCCCCCCHHHCCCHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
APQLSGFVTGQDFIVDGGMSRKMHYA
HHHHHCEECCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MIPAISAWNPSPLQDRVVVITGGAQGIGRGIAQAVLGAGGSVVIGDLDADAGKACLQEWA
CCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHC
LPRRSAFVRCDAARQAQATRLVATALKRFGRIDGLVNNAGVPDPRIAALPQLDWDTWNSR
CCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHH
LSSLHGAFLCSKQALPALRQAPAGGAIVNIASTRAWQSEPHSEAYAAAKGGLVAFTHALA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
LSEGPHVRVNSISPGWISTDAWRAPQRRRAPKLSRRDHAQHPAGRVGTPEDIAQLAVYLL
HCCCCCEEEECCCCCCCCCHHHCCCHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
APQLSGFVTGQDFIVDGGMSRKMHYA
HHHHHCEECCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934833; 10984043 [H]