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The map label for this gene is dctA

Identifier: 21244196

GI number: 21244196

Start: 4100954

End: 4102327

Strand: Reverse

Name: dctA

Synonym: XAC3471

Alternate gene names: 21244196

Gene position: 4102327-4100954 (Counterclockwise)

Preceding gene: 21244201

Following gene: 77748718

Centisome position: 79.26

GC content: 64.48

Gene sequence:

>1374_bases
ATGCACATCAGCAAGCCTGCCGGCCCCCTGCCGGCGTCCGTCCCCTTCTATCGCCAACTGTATTTTCAGGTGGTGGTGGC
GATCGTCCTCGGTGCCCTGCTGGGCCATTTCGAGCCCGCCTTCGCCGAAAGCCTCAAGCCGCTGGGCGACGCCTTCATCA
AGCTGGTGAAGATGATCATCGCGCCGGTGATCTTCCTGACCATCGTCACCGGCATCGCCGGCATGACCCATCTCAAGACG
GTGGGCCGGGTGTTCGGCAAGGCGATGGCCTACTTCCTGTTCTTTTCCACGTTGGCCCTGGTGGTCGGTCTGGTCGTTGC
CCACGTGGTGCAGCCCGGCGCCGGCATGAACATCAATCCGGCCGATCTGGACCAGAGTGCGGTCAAGAGCTATGTCGAGA
AGTCGCATGACCTGACCCTGGTCGGCTTCCTGATGGACATCATCCCCAACTCGCTGATCGGCGCATTCACCGGCGATCAG
GTCGTCAACGGCAAGCTGACCGGGCCGAACATCCTGCAGGTGCTGTTCGTGGCGGTGCTGTTCGGGGTGTCGCTGGCGTT
GGTGGGAGAGCGCGGCAAGCCGGTGCTGAACCTGCTGGAAGCGCTGATCGCGCCGGTGTTCAAGCTGGTGCACATCCTGA
TGCGTGCCGCCCCGATCGGTGCCTTCGGCGCCATCGCCTTCACCATCGGCAAGTACGGCGTGGAGTCGCTGGTCAACCTG
GCCTGGCTGGTGGGCTCGTTTTATCTGACATCCCTGTTGTTCGTGCTGGTGATTCTGGGGCTGGTGAGCCGGCTATGCGG
GTTTTCGGTACTCAAGCTGATCCGCTACCTGAAGGCCGAGCTGCTGCTGGTGCTGGGCACGTCCTCGTCGGAGTCGGCGT
TGCCGTCGCTGATGGAGAAGATGGAAAAGGCCGGTTGCGAGAAGTCGGTGGTGGGCCTGGTGGTGCCGACCGGGTACTCG
TTCAATCTGGACGGCACCAACATCTACATGACGCTGGCGGCGCTGTTCATCGCCCAGGCCACCAATACCGAACTGACCCT
GGGCCACCAGATCGCACTGCTGGCCGTGGCGATGCTCAGCTCCAAGGGCGCGGCCGGCGTCACCGGTGCCGGCTTCATCA
CCCTGGCCGCCACGCTGGCGGTGGTGCCCGAAGTGCCGGTGGCCGGCATGGCGCTGATTCTGGGCGTGGACCGTTTCATG
AGCGAATGCCGCTCGCTGACCAACTTCATCGGCAACGCAGTGGCCACCGTGGTGGTCTCGCGCTGGGAAAACGCCCTGGA
CCGCGACCGGCTCAAGCTGGTGCTGGACGGCGGCGAACCACCGCTGCTGGCCCCGGTCGGCCAACCCGGCGTGGCACCTG
CCAGCCTGCGTTGA

Upstream 100 bases:

>100_bases
GGTAGTACCAATGGGCTATCGGCCGGGGCTGTGACGTGCGGCCAGAATGCGTGCAACGGGTGCCTGCTCGGCACCCTCTC
TCGTCTCTCGGAGTGGTGCC

Downstream 100 bases:

>100_bases
TGCTTCCTACGGCCCTGTCTTCCGTCGGTGTTAACGACGGGATGCAGGGCCGTTCTTGTTTGGCCGCCACGCCGGCTGCG
CCGCCCGCCACGCCGCATCC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MHISKPAGPLPASVPFYRQLYFQVVVAIVLGALLGHFEPAFAESLKPLGDAFIKLVKMIIAPVIFLTIVTGIAGMTHLKT
VGRVFGKAMAYFLFFSTLALVVGLVVAHVVQPGAGMNINPADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQ
VVNGKLTGPNILQVLFVAVLFGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
AWLVGSFYLTSLLFVLVILGLVSRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEKMEKAGCEKSVVGLVVPTGYS
FNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFM
SECRSLTNFIGNAVATVVVSRWENALDRDRLKLVLDGGEPPLLAPVGQPGVAPASLR

Sequences:

>Translated_457_residues
MHISKPAGPLPASVPFYRQLYFQVVVAIVLGALLGHFEPAFAESLKPLGDAFIKLVKMIIAPVIFLTIVTGIAGMTHLKT
VGRVFGKAMAYFLFFSTLALVVGLVVAHVVQPGAGMNINPADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQ
VVNGKLTGPNILQVLFVAVLFGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
AWLVGSFYLTSLLFVLVILGLVSRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEKMEKAGCEKSVVGLVVPTGYS
FNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFM
SECRSLTNFIGNAVATVVVSRWENALDRDRLKLVLDGGEPPLLAPVGQPGVAPASLR
>Mature_457_residues
MHISKPAGPLPASVPFYRQLYFQVVVAIVLGALLGHFEPAFAESLKPLGDAFIKLVKMIIAPVIFLTIVTGIAGMTHLKT
VGRVFGKAMAYFLFFSTLALVVGLVVAHVVQPGAGMNINPADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQ
VVNGKLTGPNILQVLFVAVLFGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
AWLVGSFYLTSLLFVLVILGLVSRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEKMEKAGCEKSVVGLVVPTGYS
FNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFM
SECRSLTNFIGNAVATVVVSRWENALDRDRLKLVLDGGEPPLLAPVGQPGVAPASLR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI4827012, Length=448, Percent_Identity=25.2232142857143, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194239697, Length=486, Percent_Identity=25.5144032921811, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI169790839, Length=457, Percent_Identity=26.9146608315098, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI21314632, Length=397, Percent_Identity=27.2040302267003, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI40254478, Length=448, Percent_Identity=25.6696428571429, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI66773030, Length=397, Percent_Identity=24.9370277078086, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI5032093, Length=324, Percent_Identity=28.7037037037037, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI223468566, Length=298, Percent_Identity=30.5369127516779, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI223468564, Length=282, Percent_Identity=30.4964539007092, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI262359914, Length=398, Percent_Identity=25.1256281407035, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI301601644, Length=167, Percent_Identity=35.3293413173653, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789947, Length=424, Percent_Identity=58.0188679245283, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1790514, Length=424, Percent_Identity=39.622641509434, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1788024, Length=414, Percent_Identity=24.8792270531401, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537407, Length=422, Percent_Identity=28.1990521327014, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71996953, Length=448, Percent_Identity=28.3482142857143, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=458, Percent_Identity=27.292576419214, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71983099, Length=458, Percent_Identity=27.292576419214, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI193206654, Length=449, Percent_Identity=22.71714922049, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193206505, Length=420, Percent_Identity=23.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=399, Percent_Identity=23.0576441102757, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583025, Length=441, Percent_Identity=24.4897959183673, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17137668, Length=441, Percent_Identity=24.4897959183673, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583023, Length=441, Percent_Identity=24.4897959183673, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281360481, Length=422, Percent_Identity=23.696682464455, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360483, Length=422, Percent_Identity=23.696682464455, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137666, Length=422, Percent_Identity=23.696682464455, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_XANAC (Q8PGZ1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643778.1
- ProteinModelPortal:   Q8PGZ1
- SMR:   Q8PGZ1
- GeneID:   1157542
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3471
- HOGENOM:   HBG739804
- OMA:   NAVACIV
- ProtClustDB:   PRK01663
- BioCyc:   XAXO190486:XAC3471-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 48108; Mature: 48108

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x126ec3fc)-; HASH(0x123e284c)-; HASH(0xb3eec44)-; HASH(0x128671e0)-; HASH(0x1261fb58)-; HASH(0x40927474)-; HASH(0x12913224)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCHHHEEEEEECCCCCEECCCCCCCCCCCCCH
>Mature Secondary Structure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HHHHHHCHHHEEEEEECCCCCEECCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217