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The map label for this gene is mpl [H]

Identifier: 21244161

GI number: 21244161

Start: 4045771

End: 4047129

Strand: Reverse

Name: mpl [H]

Synonym: XAC3436

Alternate gene names: 21244161

Gene position: 4047129-4045771 (Counterclockwise)

Preceding gene: 21244162

Following gene: 21244160

Centisome position: 78.2

GC content: 66.81

Gene sequence:

>1359_bases
ATGAGCAAACTCCACATTCTCGGCATCGCCGGAACTTTCATGGGCGGTGTGGCCGCCCTGGCGCGTGAGCTGGGCTGGCA
GGTGGAAGGCAGCGACCAGGCCATCTATCCGCCGATGTCCACACAACTGGAAACGCTGGGCATTGCGCTGGCACAGGGAT
ACGCGCCGTCCAATATTTCTCTGGACGCGACCGAGGTGGTTGTCGGTAATGCCCTGTCGCGCCGCAATCCGGCGGTGGAG
GCGGTGCTGGATGCCGGCCGCCGGTACACCTCCGGCGCGCAGTGGCTGGCCGAGCAGGTGCTGCCGGGCCGCGACACGCT
GGCGGTCGCCGGCACCCATGGCAAGACCACCACCACCACCATCCTGAGCTACCTGCTGGAGGCGGCCGGGCGCGCGCCGG
GGTTTCTGATCGGTGGCGTGGCCGAAGATTTCGGCGTGTCGGCGCGACTGGGCCAGGGGCGCGAATTCGTGGTGGAAGCC
GACGAATACGACACCGCGTTTTTCGACAAGCGCAGCAAGTTCGTGCACTACCGGCCATTGGTGGCGATCCTCAATAACCT
GGAATACGACCACGCCGACATCTTCCCGGACGTGGCCGCCATCCAGCGCCAGTTTCACCATCTGGTGCGCACGGTGCCCG
CGCGCGGTCGCCTGATCGTCAACGGCGAAGACGCGCACCTGGCCGAGGTGCTGGCGATGGGATGCTGGACGCCGGTGGAG
CGGTTCGGCTTCGAGGCGTCGCTGGACTGGAGTGCGCGTTTGATCGCGGCCGATGGCAGCGCATTTGCGGTGCTGCATCG
TGGCGTGGAAATTGGCCAGGTGCAGTGGCCGCTGGTTGGGCGGCACAACGTCCTGAACGGGTTGGCGGCGCTGGCGGCGG
TGCAGGCGGTGGGTGTGGATCCGGCGACGGTGATGCCGGCACTGGCGCAGTTCCAGAGCGTCAAGCGGCGGCTGGAAGTC
ATTGGCAACGCGCGCGACATCACCGTGTACGACGACTTCGCGCACCATCCCACCGCCATCGCGACCACGTTGCAGGGCTT
GCGTGCGAAGGTCGGGGCGGCGCGTGTTGTGGTGGCAATGGAGCCGCGCAGCAATTCGATGCGGCTGGGCGCGCATGCGC
AGGCACTGGCGCCGTCGTTGCACGATGCCGATGCAGTGGTGTTTTTACATCGGCCGGAACTGGCCTGGGACGCCGCACCC
ATCATTGCGCAGGTGCGCGGCGAGGCACGCGTGGTGCAGGACGTGGATGCCTTGCTGCACACGCTGGGCGAGATTGCCCG
GCCCGGCGACCATGTGGTGTTCATGTCCAATGGCGGCTTCGATGGTGCGCCGCGTCGCTTCCTGGCGCAGTTGTCCTGA

Upstream 100 bases:

>100_bases
ATCGGGAAATTCCCGACAGTACGGTAGGAAATCCCCGACACCTGGGCTTGCGTTGGATCGGAATGTCCGGCAACTGCGGC
GATCGGCGACAATAGGCGGC

Downstream 100 bases:

>100_bases
TGGTGCCGCTCATGGCTACCGGCGAGACCAGCACCTTGCCGCTGTTTCCGTTGCACAGCGTGTTGCTGCCCGGTGCGGCA
ATGGGCTTGCGCGTGTTCGA

Product: UDP-N-acetylmuramate-L-alanine ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISLDATEVVVGNALSRRNPAVE
AVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEA
DEYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
RFGFEASLDWSARLIAADGSAFAVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVDPATVMPALAQFQSVKRRLEV
IGNARDITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAP
IIAQVRGEARVVQDVDALLHTLGEIARPGDHVVFMSNGGFDGAPRRFLAQLS

Sequences:

>Translated_452_residues
MSKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISLDATEVVVGNALSRRNPAVE
AVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEA
DEYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
RFGFEASLDWSARLIAADGSAFAVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVDPATVMPALAQFQSVKRRLEV
IGNARDITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAP
IIAQVRGEARVVQDVDALLHTLGEIARPGDHVVFMSNGGFDGAPRRFLAQLS
>Mature_451_residues
SKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISLDATEVVVGNALSRRNPAVEA
VLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEAD
EYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVER
FGFEASLDWSARLIAADGSAFAVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVDPATVMPALAQFQSVKRRLEVI
GNARDITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPI
IAQVRGEARVVQDVDALLHTLGEIARPGDHVVFMSNGGFDGAPRRFLAQLS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=52.549889135255, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786279, Length=354, Percent_Identity=34.180790960452, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48392; Mature: 48261

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNIS
CCCEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
LDATEVVVGNALSRRNPAVEAVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTT
CCHHHHHHCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
ILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEADEYDTAFFDKRSKFVHYRPL
HHHHHHHHHCCCCCEEEECCHHHCCCCEECCCCCEEEEECCCCCHHHHHCCCCEEEHHHH
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHH
RFGFEASLDWSARLIAADGSAFAVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVD
HCCCCCCCCCCEEEEEECCCEEEHHHCCCEECEEECCCCCCHHHHHHHHHHHHHHHCCCC
PATVMPALAQFQSVKRRLEVIGNARDITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAM
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
EPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPIIAQVRGEARVVQDVDALLH
CCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
TLGEIARPGDHVVFMSNGGFDGAPRRFLAQLS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNIS
CCEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
LDATEVVVGNALSRRNPAVEAVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTT
CCHHHHHHCCHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
ILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEADEYDTAFFDKRSKFVHYRPL
HHHHHHHHHCCCCCEEEECCHHHCCCCEECCCCCEEEEECCCCCHHHHHCCCCEEEHHHH
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHH
RFGFEASLDWSARLIAADGSAFAVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVD
HCCCCCCCCCCEEEEEECCCEEEHHHCCCEECEEECCCCCCHHHHHHHHHHHHHHHCCCC
PATVMPALAQFQSVKRRLEVIGNARDITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAM
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
EPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPIIAQVRGEARVVQDVDALLH
CCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
TLGEIARPGDHVVFMSNGGFDGAPRRFLAQLS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]