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The map label for this gene is spoT [H]

Identifier: 21244118

GI number: 21244118

Start: 3999812

End: 4001983

Strand: Reverse

Name: spoT [H]

Synonym: XAC3393

Alternate gene names: 21244118

Gene position: 4001983-3999812 (Counterclockwise)

Preceding gene: 21244119

Following gene: 21244117

Centisome position: 77.32

GC content: 64.69

Gene sequence:

>2172_bases
ATGAACCCAGGCCCCACTGCCCAGGCGACCGTCGTGACGTCTCCGTCTTCCGACCAGGCCATCCCCGACTACGTCCTGCA
TCTCGAACGCGCGGCCAGCTACCTGCCCAAGGAGCAGCTGCCGATCCTGCGGCGTGCCTGGGAAGTGGGTGCCACCGCGC
ATGCCGGGCAGACCCGCAAGTCGGGCGAGCCGTACATCACCCACCCGGTGGCGGTGGCTGGCGTGCTGGCCGAGCTGGGC
CTGGACATGGAATCGCTGATCGCCGCGATCCTGCACGACACCATTGAAGATACGCCGCTGACGCGCGAAGAACTGGCGTC
CGAGTTCGGCGAAGCAGTGGCCGAGCTGGTCGATGGCGTCACCAAGCTGGACAAGCTCAAGTTCCGCGACCGTCAGGAAG
CGGCGGCGGAAAGTTTCCGCAAGATGCTGCTGGCGATGTCGCGCGATCTGCGCGTGATCATGATCAAGCTGGCCGACCGC
CTGCACAACATGCGCACGCTCGGCGCGCAGAGCACCGAGGCGCGCGGCCGCATCGCACGCGAGACGCTGGAGATCTACGC
ACCCATCGCGCAGCGCTTGGGCATGAGCCTGATCAAGTCCGAATTGCAGAATCTGGGCTTTCGCGCGTTGTATCCCTGGC
GCCACGCCATCATCGAAAAGCACATCCGCAGCCAGCCGGTGGTGCGGCGCGAATCGATGGCGCAGGTGGAAGTGCAGCTG
TCGCAGCGGCTGGCCAAGGAAGGGCTGGAGCATCGGCTGGTCAGCCGCATCAAGACGCCGTGGAGCATCTATTCCAAGAT
GCACGAAGAGAACAAATCCTTCGACCAGGTGATGGACGTGTTCGGTTTCCGGCTGGTAGTGCGTTCGGTGGCCGATTGCT
ATCACGCGCTGGGCGCAGTGCATGCCAGCTTCAAGCCGCTGGATGGGCGCTTCCGCGATTTCATCGCAATCCCTAAGGCC
AATGGCTACCAGTCGCTGCACACCGTGTTGTTCGGACCGTACGGCTCGCCGATCGAAGTACAGATCCGCACCGAAGAGAT
GGACCTGATCGCCGAACGTGGCGTGGCGGCGCACTGGACATACAAGGTCGGCTCGGCTTCGCCCAACAGCGCGCAGAGTC
GCGCGCACGACTGGATCGTGGAACTGATCGATTCGCAGCGCGCCGCCGGCTCGTCGCTGGAATTCCTGGACAACGTCAAG
GTCGACCTGTTTCCGGACGAGGTCTATCTGTTCACGCCCAAGGGCAAGATCCTGGCGTTGCCGCGCAATTCCACCGCACT
GGATTTTGCCTACGCGGTGCATACCGACGTGGGCAATCGCGCCGTGGCCTCGCGCGTGGACAAGAAGCTGGTGCCGTTGC
GCACCAAGCTGGTCAGCGGTCAGGCGGTGGAGATCATCACCGCACGCTCGGCCACGCCCAAACCGCAGTGGCTGGAATTC
GTGGTCAGCAGCAAGGCGCGCACGGCGATTCGTCACCAGCTCAAGCAACTCGAGCACGAAGATGCCGTGCAGTTGGGCCA
CCGCATGCTGGACCGCGCGCTGGAAGCGATGGACTCCTCGCTGGAGCGGCTGCCCAAGGGGCGCCTGGACGCCTTCCTCA
GCGAGCACCGCTACCCGCGTCTGGAAGCGCTGCTGGCCGATGTGGCGCTTGGCAACTGGATGCCCAATCAGGCCGCGCAG
GCGCTGATGGCTTACGCGGAATTGCGTGGCGGCGGCCTGTCCAAGCATTCGCACGAAAAGATCCTGATCGACGGCAGCGA
GCGCGGCGTGATCAGTTTCGCCAATTGCTGCCAGCCGATTCCCGGCGATGAGATCATGGGCTACCACACCGCCGGCAAGG
GCATCGTGGTGCACCGCCTGGACTGCCCGAACCTGGCGGAACTGCGCAAATCGCCCGAGCGTTGGGTGCCGATCGACTGG
GACTCCAACGTGACCGGCGATTACGACACCGCGCTGGTGGTCGAAGTGGAAAACCGCACCGGCGTGCTGGCGCAGTTGGC
GGCAGCCATCGCGCAGAGTCAGTCCAATATCGAGCGCGTGGACTACCTGGACCGCGACTTCAACGCAGCCGTGCTGCGCT
TCAACATCCAGGTGCGCGACCGCCGCCACCTGGCTGAAGTGATGCGCCGGCTGCGGCGCCTGCACGTGGTGCAGAGCGTC
GGGCGCCAGTAG

Upstream 100 bases:

>100_bases
CAGCCCGCCTCGACGCGGGCTGTTTTCGTTTTCGGTCCCGGCAATACGCCTGCGTGTGCAAATGTTTGCCGAGACTGCAC
GGCTGGCATAGTCTTCCGGC

Downstream 100 bases:

>100_bases
CGCCTGGCGCGCTCGCGCAACAGTCGAGCAAATCCGCACAAAGGCCTAAACTGCGGCAACCCTTTGCAGTGGAGTTGCCA
TGTCCCAGATCATCCATACC

Product: pentaphosphate guanosine-3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRSVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLSEHRYPRLEALLADVALGNWMPNQAAQ
ALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSNVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ

Sequences:

>Translated_723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRSVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLSEHRYPRLEALLADVALGNWMPNQAAQ
ALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSNVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ
>Mature_723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRSVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLSEHRYPRLEALLADVALGNWMPNQAAQ
ALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSNVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=137, Percent_Identity=37.2262773722628, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1790082, Length=695, Percent_Identity=48.0575539568345, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=687, Percent_Identity=33.0422125181951, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25143118, Length=129, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80849; Mature: 80849

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRK
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCCCCCC
SGEPYITHPVAVAGVLAELGLDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGV
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
TKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSTEARGRIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRSVADCYHALGAV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HASFKPLDGRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWT
HHCCCCCCCCHHHEEECCCCCCHHHHHHHEECCCCCCEEEEEEHHHHHHHHHCCCEEEEE
YKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVKVDLFPDEVYLFTPKGKILAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEE
PRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLSEHRYPR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LEALLADVALGNWMPNQAAQALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDWDSNVTGDYDTALVVEVENRT
CCCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
GRQ
CCC
>Mature Secondary Structure
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGATAHAGQTRK
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCCCCCC
SGEPYITHPVAVAGVLAELGLDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGV
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
TKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSTEARGRIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRSVADCYHALGAV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HASFKPLDGRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWT
HHCCCCCCCCHHHEEECCCCCCHHHHHHHEECCCCCCEEEEEEHHHHHHHHHCCCEEEEE
YKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVKVDLFPDEVYLFTPKGKILAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEE
PRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLSEHRYPR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LEALLADVALGNWMPNQAAQALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDWDSNVTGDYDTALVVEVENRT
CCCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
GRQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]