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The map label for this gene is gshB

Identifier: 21243830

GI number: 21243830

Start: 3646078

End: 3647028

Strand: Direct

Name: gshB

Synonym: XAC3103

Alternate gene names: 21243830

Gene position: 3646078-3647028 (Clockwise)

Preceding gene: 77748695

Following gene: 21243831

Centisome position: 70.45

GC content: 67.93

Gene sequence:

>951_bases
ATGTCGCTCGACGTCGTTGTGGTGATGGATCCCATCGCCTCCATCAAGATCGCCAAAGACACTACGTTTGCCATGTTGCT
GGAAGCCCAGCGCCGTGGCCACCGTCTGCACTACGTGCGCCCCGGCGGGCTCAGCCTGCGCGAAGGGCGCGCGGTGGCGC
AGGTGGCGCCGCTGAGCGTGCGCGAGGACAAGACCAGCTGGTTCACGCTGGGCGAGTTCGCCGAACTGGCGTTCGGGCCC
GGCCAGGTGGTGCTGATGCGCAAGGACCCGCCGGTGGATGCGGAGTTCGTCTACGACACCCAGGTCCTGAGCGTGGCGCA
GCGCGCCGGCGCGCAGATCGTCAACGACCCGCAGGGACTGCGCGACTACAACGAGAAGCTGGCCGCACTGCTGTTTCCGC
AATGCTGCCCGCCGACCCTGGTCAGCCGCGATGCCGCCGCGCTCAAGGCCTTCGTGCTGGAACATGGCCAGGCGGTGCTC
AAGCCGCTCGATGGCATGGGCGGACGCTCGATCTTCCGCAGCGGCAGCGGCGACCCCAACCTCAACGTGATCCTGGAAAC
GCTGACCGACGGCAACCGCAAACTGACCCTGGCGCAGCGCTTCATCCCCGACATCACTGCCGGCGACAAGCGCATCCTGC
TGGTGGACGGCTTGCCGGTGGATTACTGCCTGGCGCGGATTCCGCAGGGCGACGAATTCCGCGGCAACCTGGCCGCCGGC
GGGCGCGGCGAAGGCCGGCCATTGTCCGAACGCGACCGCTGGATCGCCGCGCAGGTAGGCCCGGAAATGCGCCGTCGCGG
CATGCGCTTCGTCGGCCTGGACGTGATCGGCGATTACCTGACCGAGGTCAACGTCACCAGCCCCACCTGCGTGCGCGAAC
TGGATGCGCAGTTCGGCCTGAACATCGCCGGGCTGCTGTTCGATGCGATCGAGGCCGGCGCCGCGCAATGA

Upstream 100 bases:

>100_bases
GTGCGTCCCGGCCGGCAAGTCGCGCACTACGACCCGCCGGGCGCGCCATCGCCCATCGCTTGAAGTTACCATCGGCAGCC
TGTTCGAAAGGTTTAGCCCC

Downstream 100 bases:

>100_bases
GCACGGCCGCGGCCCTGCCGGCACCGATGGACGACGGTCGCCGGCTGATGATGACGCTGGTGATCTCGCTGCTGCTGCAT
GGCGTGCTGATCCTGGGGGT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLREGRAVAQVAPLSVREDKTSWFTLGEFAELAFGP
GQVVLMRKDPPVDAEFVYDTQVLSVAQRAGAQIVNDPQGLRDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLEHGQAVL
KPLDGMGGRSIFRSGSGDPNLNVILETLTDGNRKLTLAQRFIPDITAGDKRILLVDGLPVDYCLARIPQGDEFRGNLAAG
GRGEGRPLSERDRWIAAQVGPEMRRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGLNIAGLLFDAIEAGAAQ

Sequences:

>Translated_316_residues
MSLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLREGRAVAQVAPLSVREDKTSWFTLGEFAELAFGP
GQVVLMRKDPPVDAEFVYDTQVLSVAQRAGAQIVNDPQGLRDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLEHGQAVL
KPLDGMGGRSIFRSGSGDPNLNVILETLTDGNRKLTLAQRFIPDITAGDKRILLVDGLPVDYCLARIPQGDEFRGNLAAG
GRGEGRPLSERDRWIAAQVGPEMRRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGLNIAGLLFDAIEAGAAQ
>Mature_315_residues
SLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLREGRAVAQVAPLSVREDKTSWFTLGEFAELAFGPG
QVVLMRKDPPVDAEFVYDTQVLSVAQRAGAQIVNDPQGLRDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLEHGQAVLK
PLDGMGGRSIFRSGSGDPNLNVILETLTDGNRKLTLAQRFIPDITAGDKRILLVDGLPVDYCLARIPQGDEFRGNLAAGG
RGEGRPLSERDRWIAAQVGPEMRRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGLNIAGLLFDAIEAGAAQ

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=56.7741935483871, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_XANAC (Q8PHZ5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643412.1
- ProteinModelPortal:   Q8PHZ5
- SMR:   Q8PHZ5
- GeneID:   1157174
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3103
- NMPDR:   fig|190486.1.peg.3056
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BioCyc:   XAXO190486:XAC3103-MONOMER
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 34322; Mature: 34191

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLREGRAVAQVAPLSV
CCEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCEE
REDKTSWFTLGEFAELAFGPGQVVLMRKDPPVDAEFVYDTQVLSVAQRAGAQIVNDPQGL
ECCCCCEEEHHHHHHHCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCEECCCCHHH
RDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLEHGQAVLKPLDGMGGRSIFRSGSGDPN
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHCCCCCCCC
LNVILETLTDGNRKLTLAQRFIPDITAGDKRILLVDGLPVDYCLARIPQGDEFRGNLAAG
CEEEEEEECCCCEEEEEHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGEGRPLSERDRWIAAQVGPEMRRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGL
CCCCCCCCCHHHCEEEECCCHHHHHCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCC
NIAGLLFDAIEAGAAQ
CHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLDVVVVMDPIASIKIAKDTTFAMLLEAQRRGHRLHYVRPGGLSLREGRAVAQVAPLSV
CEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCEE
REDKTSWFTLGEFAELAFGPGQVVLMRKDPPVDAEFVYDTQVLSVAQRAGAQIVNDPQGL
ECCCCCEEEHHHHHHHCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCEECCCCHHH
RDYNEKLAALLFPQCCPPTLVSRDAAALKAFVLEHGQAVLKPLDGMGGRSIFRSGSGDPN
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHCCCCCCCC
LNVILETLTDGNRKLTLAQRFIPDITAGDKRILLVDGLPVDYCLARIPQGDEFRGNLAAG
CEEEEEEECCCCEEEEEHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
GRGEGRPLSERDRWIAAQVGPEMRRRGMRFVGLDVIGDYLTEVNVTSPTCVRELDAQFGL
CCCCCCCCCHHHCEEEECCCHHHHHCCCEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCC
NIAGLLFDAIEAGAAQ
CHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217