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The map label for this gene is 21243038

Identifier: 21243038

GI number: 21243038

Start: 2692724

End: 2693608

Strand: Direct

Name: 21243038

Synonym: XAC2303

Alternate gene names: NA

Gene position: 2692724-2693608 (Clockwise)

Preceding gene: 21243037

Following gene: 21243039

Centisome position: 52.03

GC content: 66.33

Gene sequence:

>885_bases
ATGACCCGTCATCTCCTTCCCGTCGCGCTGATCCAGGAGCGCAACCACGGCGACGCCGAGGCCAACCTGGCCGTCATCGC
ATCGCGCGTGGCCGAAGCCGCCGCGCAGGGCGCCAAGCTGGTGCTGCTGCAGGAACTGCATAACGGTGCGTATTTCTGCC
AGCACGAGTCGGTGGACGAATTCAACCTGGCCGAGCCGATTCCCGGCCCCAGCACCGAGCGTCTGAGCGCGCTGGCCAAG
CAGCACGGAGTGGTGCTGGTCGCATCGCTGTTCGAACGCCGCGCGGCCGGCCTGTATCACAACACCGCGGTGGTGTTCGA
AAAGGACGGCCGCCTGCTCGGCAAGTACCGCAAGATGCACATCCCCGACGATCCGGGCTTCTACGAGAAGTTCTATTTCA
CTCCGGGCGATCTGGGCTTCACGCCGATCGATACCTCGGTGGGCCGCCTTGGCGTGCTGGTGTGCTGGGACCAGTGGTAT
CCGGAGGCGGCGCGCCTGATGGCGCTGGCCGGTGCGGAGCTGCTGCTCTACCCCACGGCGATCGGTTGGGATCCGGACGA
CCAGCAGCCGGAGCAGGAGCGCCAGCGCGATGCCTGGATCCTCAGCCATCGCGGCCATGCGGTCGCCAACGGCGTGCCGG
TACTGTCGTGCAACCGGGTCGGCCATGAGCCCTCCCCGCTGGGCGCATCGGGCATCCAGTTCTGGGGCAACAGCCATGTG
CTGGGGCCGCAGGGCGAGTTCATTGCCGAAGCCGGCCAGGACCCGACCGTACTGATCTGCGATGTCGACCTGCAACGCAG
CGAACATGTGCGCCGCATTTGGCCATTCCTGCGCGACCGTCGCATCGATGCGTACGGCGATCTGCTCAAGCGCTACATCG
ACTGA

Upstream 100 bases:

>100_bases
TTGCGGCCGCACAGACCGCGCGTGGCTCCTGTTCCGGCCGGCAGGCCGGTGTTACGCCATGGCGCGCTACCTTAGTGCGT
TACTTACGTTGGATGCTGCC

Downstream 100 bases:

>100_bases
CGCATGCCACTCGAGTTGCGTGCGGTCCGCAATGCGGACATTCCGGCGATCACTGCGATCTATGCGGAGCAGGTCGCCGG
GGTCAATACCTACGAATACA

Product: beta-alanine synthetase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTRHLLPVALIQERNHGDAEANLAVIASRVAEAAAQGAKLVLLQELHNGAYFCQHESVDEFNLAEPIPGPSTERLSALAK
QHGVVLVASLFERRAAGLYHNTAVVFEKDGRLLGKYRKMHIPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQWY
PEAARLMALAGAELLLYPTAIGWDPDDQQPEQERQRDAWILSHRGHAVANGVPVLSCNRVGHEPSPLGASGIQFWGNSHV
LGPQGEFIAEAGQDPTVLICDVDLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID

Sequences:

>Translated_294_residues
MTRHLLPVALIQERNHGDAEANLAVIASRVAEAAAQGAKLVLLQELHNGAYFCQHESVDEFNLAEPIPGPSTERLSALAK
QHGVVLVASLFERRAAGLYHNTAVVFEKDGRLLGKYRKMHIPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQWY
PEAARLMALAGAELLLYPTAIGWDPDDQQPEQERQRDAWILSHRGHAVANGVPVLSCNRVGHEPSPLGASGIQFWGNSHV
LGPQGEFIAEAGQDPTVLICDVDLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID
>Mature_293_residues
TRHLLPVALIQERNHGDAEANLAVIASRVAEAAAQGAKLVLLQELHNGAYFCQHESVDEFNLAEPIPGPSTERLSALAKQ
HGVVLVASLFERRAAGLYHNTAVVFEKDGRLLGKYRKMHIPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQWYP
EAARLMALAGAELLLYPTAIGWDPDDQQPEQERQRDAWILSHRGHAVANGVPVLSCNRVGHEPSPLGASGIQFWGNSHVL
GPQGEFIAEAGQDPTVLICDVDLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=289, Percent_Identity=33.5640138408304, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI297632348, Length=291, Percent_Identity=27.8350515463918, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI297632350, Length=291, Percent_Identity=27.8350515463918, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI5031947, Length=291, Percent_Identity=27.8350515463918, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI7706509, Length=280, Percent_Identity=29.6428571428571, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=280, Percent_Identity=30, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556280, Length=292, Percent_Identity=25, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17557111, Length=258, Percent_Identity=26.7441860465116, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323383, Length=299, Percent_Identity=31.1036789297659, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322335, Length=318, Percent_Identity=25.4716981132075, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358471, Length=288, Percent_Identity=29.8611111111111, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355835, Length=293, Percent_Identity=26.6211604095563, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI17933642, Length=286, Percent_Identity=25.8741258741259, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32717; Mature: 32586

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHLLPVALIQERNHGDAEANLAVIASRVAEAAAQGAKLVLLQELHNGAYFCQHESVDE
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCEEEEECCCCCC
FNLAEPIPGPSTERLSALAKQHGVVLVASLFERRAAGLYHNTAVVFEKDGRLLGKYRKMH
CCCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHCCCEECEEEEEECCCCHHHHHHCCC
IPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQWYPEAARLMALAGAELLLYPTA
CCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEE
IGWDPDDQQPEQERQRDAWILSHRGHAVANGVPVLSCNRVGHEPSPLGASGIQFWGNSHV
CCCCCCCCCCHHHHHHHHEEEECCCCCHHCCCCEEEECCCCCCCCCCCCCCCEECCCCEE
LGPQGEFIAEAGQDPTVLICDVDLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID
ECCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TRHLLPVALIQERNHGDAEANLAVIASRVAEAAAQGAKLVLLQELHNGAYFCQHESVDE
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCEEEEECCCCCC
FNLAEPIPGPSTERLSALAKQHGVVLVASLFERRAAGLYHNTAVVFEKDGRLLGKYRKMH
CCCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHCCCEECEEEEEECCCCHHHHHHCCC
IPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQWYPEAARLMALAGAELLLYPTA
CCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEE
IGWDPDDQQPEQERQRDAWILSHRGHAVANGVPVLSCNRVGHEPSPLGASGIQFWGNSHV
CCCCCCCCCCHHHHHHHHEEEECCCCCHHCCCCEEEECCCCCCCCCCCCCCCEECCCCEE
LGPQGEFIAEAGQDPTVLICDVDLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID
ECCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]