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The map label for this gene is yadF [H]

Identifier: 21242354

GI number: 21242354

Start: 1853155

End: 1853817

Strand: Reverse

Name: yadF [H]

Synonym: XAC1604

Alternate gene names: 21242354

Gene position: 1853817-1853155 (Counterclockwise)

Preceding gene: 21242365

Following gene: 21242353

Centisome position: 35.82

GC content: 62.14

Gene sequence:

>663_bases
ATGAACGAGCTTAACCACTTGCTTGAAAACAACCGCGCCTGGTCCGAGCGTATCCGCCGCGAGGATCCGGAATTCTTTTC
CAAACTCTCGACTCAACAGACGCCGGAATATCTGTGGATCGGCTGCTCCGATTCGCGCGTGCCGGCCAACCAGATCATCG
ACATGGCGCCGGGCGAAGTGTTCGTGCACCGGAATATCGCCAACGTGGTGGTGCATACCGACCTGAATTGCCTGTCGGTG
ATCCAGTTCGCGGTGGATGTGCTCAAGGTCAAGCATGTGCTGGTGGTCGGCCATTACGGCTGCGGCGGCGTGTTCGCCAG
CCTGACCCAGAAGCGCATGGGGCTGGTCGACAACTGGATCCGCCATGTCACCGACGTGGCCGACAAGCACGAAGCCTGCC
TGCACAACGCGGGCGAACTGTCGGTGCAGCACGCGCGCCTGTGCGAGCTCAACGTGCTCGAACAAGTGGTCAACGTGTGC
CGCACCACCATCGTGCATGACGCCTGGGAGCGTGGCCAGCAATTGAGCGTGCATGGCTGGGTATACAGCCTGCGCGATGG
CCGCGCGCACGACCTGGGCATGTCGATCGACCGCCCCGAGCTGCTGCAGGAACGCTATGACGCAGCGCTGGCGCAGATCC
AGGCCGATCAAACCGAGCGCTGA

Upstream 100 bases:

>100_bases
CGGACATCGGTCGTTCGCCGAGACTGGGAAGCCTGAAAGCTGATTCATCCTGGGCGCTCATCGAAGCCGCCACGACCACA
TTTTCTGGAATACTGCGCGC

Downstream 100 bases:

>100_bases
GCCGTACGCACACGTGGCCGGCCGCAGCGTCCATCGCACATCGGCGGCCGACTGAACCGGATATCTTCTTTGCCGAGAAA
CGTCGATGCTGACCCCGCCA

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 2 [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNELNHLLENNRAWSERIRREDPEFFSKLSTQQTPEYLWIGCSDSRVPANQIIDMAPGEVFVHRNIANVVVHTDLNCLSV
IQFAVDVLKVKHVLVVGHYGCGGVFASLTQKRMGLVDNWIRHVTDVADKHEACLHNAGELSVQHARLCELNVLEQVVNVC
RTTIVHDAWERGQQLSVHGWVYSLRDGRAHDLGMSIDRPELLQERYDAALAQIQADQTER

Sequences:

>Translated_220_residues
MNELNHLLENNRAWSERIRREDPEFFSKLSTQQTPEYLWIGCSDSRVPANQIIDMAPGEVFVHRNIANVVVHTDLNCLSV
IQFAVDVLKVKHVLVVGHYGCGGVFASLTQKRMGLVDNWIRHVTDVADKHEACLHNAGELSVQHARLCELNVLEQVVNVC
RTTIVHDAWERGQQLSVHGWVYSLRDGRAHDLGMSIDRPELLQERYDAALAQIQADQTER
>Mature_220_residues
MNELNHLLENNRAWSERIRREDPEFFSKLSTQQTPEYLWIGCSDSRVPANQIIDMAPGEVFVHRNIANVVVHTDLNCLSV
IQFAVDVLKVKHVLVVGHYGCGGVFASLTQKRMGLVDNWIRHVTDVADKHEACLHNAGELSVQHARLCELNVLEQVVNVC
RTTIVHDAWERGQQLSVHGWVYSLRDGRAHDLGMSIDRPELLQERYDAALAQIQADQTER

Specific function: Unknown

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786318, Length=216, Percent_Identity=48.1481481481481, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1786534, Length=169, Percent_Identity=31.9526627218935, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324292, Length=192, Percent_Identity=31.7708333333333, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 25024; Mature: 25024

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELNHLLENNRAWSERIRREDPEFFSKLSTQQTPEYLWIGCSDSRVPANQIIDMAPGEV
CCHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCE
FVHRNIANVVVHTDLNCLSVIQFAVDVLKVKHVLVVGHYGCGGVFASLTQKRMGLVDNWI
EEECCHHHEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHH
RHVTDVADKHEACLHNAGELSVQHARLCELNVLEQVVNVCRTTIVHDAWERGQQLSVHGW
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHE
VYSLRDGRAHDLGMSIDRPELLQERYDAALAQIQADQTER
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNELNHLLENNRAWSERIRREDPEFFSKLSTQQTPEYLWIGCSDSRVPANQIIDMAPGEV
CCHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCE
FVHRNIANVVVHTDLNCLSVIQFAVDVLKVKHVLVVGHYGCGGVFASLTQKRMGLVDNWI
EEECCHHHEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHH
RHVTDVADKHEACLHNAGELSVQHARLCELNVLEQVVNVCRTTIVHDAWERGQQLSVHGW
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHE
VYSLRDGRAHDLGMSIDRPELLQERYDAALAQIQADQTER
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 10493123 [H]