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The map label for this gene is rnt [H]

Identifier: 21242321

GI number: 21242321

Start: 1817163

End: 1817804

Strand: Reverse

Name: rnt [H]

Synonym: XAC1571

Alternate gene names: 21242321

Gene position: 1817804-1817163 (Counterclockwise)

Preceding gene: 21242323

Following gene: 21242316

Centisome position: 35.12

GC content: 64.02

Gene sequence:

>642_bases
ATGCGGATGAACGAACCGGTCGATGCACAGCCTGCCCCCTCCTTTTTGCCGATGTCGCGCCGCTTTCGTGGCTATCTGCC
CGTGGTGGTGGACGTAGAAACCGGCGGGTTCGACTGGAACAAGCATGCACTGCTGGAGATCGCTTGCGTGCCGATCGAAA
TGGGTGCCGACGGACGCTTCTTTCCCGGCGAGACCGCCAGCGCACACCTGGTGCCGGCGCCCGGGCTGGAGATCGACCCG
AAGTCGCTGGAAATCACCGGTATCGTGCTCGACCATCCATTCCGATTCGCGAAGCAGGAAAAGGAAGCGCTCGACCATGT
GTTCGCGCCGGTGCGCGCAGCAGTGAAGAAATACGGCTGCCAGCGCGCAATCCTGGTCGGCCACAACGCGCATTTCGATC
TGAATTTTCTCAACGCGGCCGTGGCGCGCGTGGGCCACAAACGCAACCCGTTTCATCCCTTCAGCGTGTTCGACACGGTG
ACGTTGGCCGGCGTGGCCTACGGCCAGACCGTGCTGGCACGCGCCGCCCAGGCCGCAGGCCTGGACTGGAATGCAGCCGA
CGCCCACAGCGCGGTCTACGACACCGAACAGACCGCACGCCTGTTCTGCAAGATCGCCAACGCCTGGCCCGGGCCGGTTT
GA

Upstream 100 bases:

>100_bases
GTGTTAGGTCATGCACCGGGTGATGCGGGCCGCAGCCGCCGCAGGTATGAATCTGCAAGCCTGCAGCCAAGGCAGAGCCC
GACGTGCGACCTGCTAGGCT

Downstream 100 bases:

>100_bases
GTTGGGGAATCGGGAATCGGGAATCGGGAATCGCAAGAGCTTTGCACAGGTGGCACTCTAAGGTGGCGCGCCAGTAGCGC
ATCTACCTCCTCTGCCTGCG

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRMNEPVDAQPAPSFLPMSRRFRGYLPVVVDVETGGFDWNKHALLEIACVPIEMGADGRFFPGETASAHLVPAPGLEIDP
KSLEITGIVLDHPFRFAKQEKEALDHVFAPVRAAVKKYGCQRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTV
TLAGVAYGQTVLARAAQAAGLDWNAADAHSAVYDTEQTARLFCKIANAWPGPV

Sequences:

>Translated_213_residues
MRMNEPVDAQPAPSFLPMSRRFRGYLPVVVDVETGGFDWNKHALLEIACVPIEMGADGRFFPGETASAHLVPAPGLEIDP
KSLEITGIVLDHPFRFAKQEKEALDHVFAPVRAAVKKYGCQRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTV
TLAGVAYGQTVLARAAQAAGLDWNAADAHSAVYDTEQTARLFCKIANAWPGPV
>Mature_213_residues
MRMNEPVDAQPAPSFLPMSRRFRGYLPVVVDVETGGFDWNKHALLEIACVPIEMGADGRFFPGETASAHLVPAPGLEIDP
KSLEITGIVLDHPFRFAKQEKEALDHVFAPVRAAVKKYGCQRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTV
TLAGVAYGQTVLARAAQAAGLDWNAADAHSAVYDTEQTARLFCKIANAWPGPV

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=192, Percent_Identity=50.5208333333333, Blast_Score=198, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 23213; Mature: 23213

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMNEPVDAQPAPSFLPMSRRFRGYLPVVVDVETGGFDWNKHALLEIACVPIEMGADGRF
CCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEEEEEECCCCCC
FPGETASAHLVPAPGLEIDPKSLEITGIVLDHPFRFAKQEKEALDHVFAPVRAAVKKYGC
CCCCCCCCEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTLAGVAYGQTVLARAAQAAG
CEEEEEECCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDWNAADAHSAVYDTEQTARLFCKIANAWPGPV
CCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRMNEPVDAQPAPSFLPMSRRFRGYLPVVVDVETGGFDWNKHALLEIACVPIEMGADGRF
CCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEEEEEECCCCCC
FPGETASAHLVPAPGLEIDPKSLEITGIVLDHPFRFAKQEKEALDHVFAPVRAAVKKYGC
CCCCCCCCEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QRAILVGHNAHFDLNFLNAAVARVGHKRNPFHPFSVFDTVTLAGVAYGQTVLARAAQAAG
CEEEEEECCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDWNAADAHSAVYDTEQTARLFCKIANAWPGPV
CCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA