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The map label for this gene is sucB [H]

Identifier: 21242285

GI number: 21242285

Start: 1772163

End: 1773374

Strand: Reverse

Name: sucB [H]

Synonym: XAC1534

Alternate gene names: 21242285

Gene position: 1773374-1772163 (Counterclockwise)

Preceding gene: 77748592

Following gene: 21242284

Centisome position: 34.26

GC content: 64.85

Gene sequence:

>1212_bases
ATGGCCACCGAAGTCAAAGTTCCGGTACTGCCCGAATCCGTTTCCGACGCCACCATCGCCAGCTGGCACAAGAAGGCCGG
CGAAGCGGTCAAGCGCGACGAGAATCTGGTCGACCTGGAAACCGACAAGGTCGTGTTGGAAGTTCCTTCGCCGGTCGACG
GCGTACTGAAGGAAATCAAGTTCGAAACCGGCAGCACGGTCACCAGCAACCAGATTCTGGCGATCATCGAAGAAGGCGCT
GTGGCTGCGGCGGCACCAGCTGAAGAGAAGCAGGCCGCTGCCCCGGCTGCGACTGCTGCGGCACCGGCTGCCGCTCCGGC
ACCGGCAGCCGCCGCGGCGCCTGCTGCTTCCAAGTCCGCTGCCGATTCGCTGCCGCCGGGCGCGCGTTTCTCCGCGATCA
CCCAGGGCGTGGATCCTTCGCAGGTCGAAGGCACCGGCCGTCGTGGCGCGGTGACCAAGGAAGACATCGTCAACTTCGCC
AAGGCCGGCGGCGTCGGCAAGGCCTCTGGTGCCCGCCCGGAAGAGCGCGTGCCGATGACCCGCGTGCGCAAGACGATCGC
CAAGCGCCTGATGGAGTCCAAGAACTCCACCGCGATGCTGACCACCTTCAATGAAGTCAACCTGGCCAAGGTGTCGGCCG
CGCGCAAGGAGCTGCAGGACGAGTTCCAGAAGGCGCACGGCATCAAGCTCGGTTTCATGAGCTTCTTCGTCAAGGCTGCC
GCCAACGCGCTGCAACGCTTCCCGCTGGTCAACGCCTCGATCGACGGCGACGACATCATCTATCACGGCTACAGCGACAT
CTCGATCGCGGTGTCGACCGACAAGGGCCTGGTCACGCCGGTGCTGCGCAACGTCGAGCGCCAGTCGTTCGCCGATGTCG
AGCAAGGCATTGCCGACTACGCCGCCAAGGCGCGCGCCGGCAAGCTGGGCCTGGACGACCTGCAGGGCGGCACCTTCACC
ATCACCAACGGCGGCACCTTCGGCTCGCTGCTGTCCACCCCGATCATCAACCCGCCGCAGAGCGCCATCCTGGGCATGCA
CGCGATCAAGGAACGTCCGATCGCCGAGAACGGCCAGGTCGTGATCGCGCCGATGATGTACCTGGCGCTGTCCTACGACC
ACCGCATCATCGACGGCAAGGACTCGGTGCAGTTCCTGGTCGACATCAAGAACCAGCTGGAAAACCCGGGCCGCATGCTG
TTCGGCCTGTAA

Upstream 100 bases:

>100_bases
GCAGAAGCTGGTCGCCGATGCGCTGCTCAACCCGTTCAACGACCAAGTCGCTGAATAACTCCTCTTCCCCAAAGAACGAA
AACCCTAGGACGCTCCCCGA

Downstream 100 bases:

>100_bases
GCGCCGGGAATCGGGAATGGAGATACGGGAATGGGACAAGCGCCCATGGACCGCTCCATTCCCGCCTGGACCTGGATGGG
ACGCCAAGGGCGCGTGCGCT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MATEVKVPVLPESVSDATIASWHKKAGEAVKRDENLVDLETDKVVLEVPSPVDGVLKEIKFETGSTVTSNQILAIIEEGA
VAAAAPAEEKQAAAPAATAAAPAAAPAPAAAAAPAASKSAADSLPPGARFSAITQGVDPSQVEGTGRRGAVTKEDIVNFA
KAGGVGKASGARPEERVPMTRVRKTIAKRLMESKNSTAMLTTFNEVNLAKVSAARKELQDEFQKAHGIKLGFMSFFVKAA
ANALQRFPLVNASIDGDDIIYHGYSDISIAVSTDKGLVTPVLRNVERQSFADVEQGIADYAAKARAGKLGLDDLQGGTFT
ITNGGTFGSLLSTPIINPPQSAILGMHAIKERPIAENGQVVIAPMMYLALSYDHRIIDGKDSVQFLVDIKNQLENPGRML
FGL

Sequences:

>Translated_403_residues
MATEVKVPVLPESVSDATIASWHKKAGEAVKRDENLVDLETDKVVLEVPSPVDGVLKEIKFETGSTVTSNQILAIIEEGA
VAAAAPAEEKQAAAPAATAAAPAAAPAPAAAAAPAASKSAADSLPPGARFSAITQGVDPSQVEGTGRRGAVTKEDIVNFA
KAGGVGKASGARPEERVPMTRVRKTIAKRLMESKNSTAMLTTFNEVNLAKVSAARKELQDEFQKAHGIKLGFMSFFVKAA
ANALQRFPLVNASIDGDDIIYHGYSDISIAVSTDKGLVTPVLRNVERQSFADVEQGIADYAAKARAGKLGLDDLQGGTFT
ITNGGTFGSLLSTPIINPPQSAILGMHAIKERPIAENGQVVIAPMMYLALSYDHRIIDGKDSVQFLVDIKNQLENPGRML
FGL
>Mature_402_residues
ATEVKVPVLPESVSDATIASWHKKAGEAVKRDENLVDLETDKVVLEVPSPVDGVLKEIKFETGSTVTSNQILAIIEEGAV
AAAAPAEEKQAAAPAATAAAPAAAPAPAAAAAPAASKSAADSLPPGARFSAITQGVDPSQVEGTGRRGAVTKEDIVNFAK
AGGVGKASGARPEERVPMTRVRKTIAKRLMESKNSTAMLTTFNEVNLAKVSAARKELQDEFQKAHGIKLGFMSFFVKAAA
NALQRFPLVNASIDGDDIIYHGYSDISIAVSTDKGLVTPVLRNVERQSFADVEQGIADYAAKARAGKLGLDDLQGGTFTI
TNGGTFGSLLSTPIINPPQSAILGMHAIKERPIAENGQVVIAPMMYLALSYDHRIIDGKDSVQFLVDIKNQLENPGRMLF
GL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=240, Percent_Identity=53.75, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=31.3364055299539, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.2222222222222, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=28.2758620689655, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.2222222222222, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.7826086956522, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786946, Length=412, Percent_Identity=54.126213592233, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=32.1782178217822, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=43.3962264150943, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=33.4883720930233, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=26.8235294117647, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=33.6283185840708, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=404, Percent_Identity=42.3267326732673, Blast_Score=316, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=27.1739130434783, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=57.4380165289256, Blast_Score=284, Evalue=6e-77,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=27.7777777777778, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=32.1739130434783, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=32.1739130434783, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42324; Mature: 42192

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEVKVPVLPESVSDATIASWHKKAGEAVKRDENLVDLETDKVVLEVPSPVDGVLKEIK
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHHHHH
FETGSTVTSNQILAIIEEGAVAAAAPAEEKQAAAPAATAAAPAAAPAPAAAAAPAASKSA
CCCCCEECCCCEEEEEECCCEEECCCCHHHHHCCCCHHHCCCCCCCCCCHHCCCCCCCHH
ADSLPPGARFSAITQGVDPSQVEGTGRRGAVTKEDIVNFAKAGGVGKASGARPEERVPMT
HCCCCCCCHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCHH
RVRKTIAKRLMESKNSTAMLTTFNEVNLAKVSAARKELQDEFQKAHGIKLGFMSFFVKAA
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ANALQRFPLVNASIDGDDIIYHGYSDISIAVSTDKGLVTPVLRNVERQSFADVEQGIADY
HHHHHHCCCEECCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAKARAGKLGLDDLQGGTFTITNGGTFGSLLSTPIINPPQSAILGMHAIKERPIAENGQV
HHHHCCCCCCCHHCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCE
VIAPMMYLALSYDHRIIDGKDSVQFLVDIKNQLENPGRMLFGL
EEHHHHHHHHHCCCEEECCCCHHHHHEEHHHHHCCCCCEECCC
>Mature Secondary Structure 
ATEVKVPVLPESVSDATIASWHKKAGEAVKRDENLVDLETDKVVLEVPSPVDGVLKEIK
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHHHHH
FETGSTVTSNQILAIIEEGAVAAAAPAEEKQAAAPAATAAAPAAAPAPAAAAAPAASKSA
CCCCCEECCCCEEEEEECCCEEECCCCHHHHHCCCCHHHCCCCCCCCCCHHCCCCCCCHH
ADSLPPGARFSAITQGVDPSQVEGTGRRGAVTKEDIVNFAKAGGVGKASGARPEERVPMT
HCCCCCCCHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHCCCHH
RVRKTIAKRLMESKNSTAMLTTFNEVNLAKVSAARKELQDEFQKAHGIKLGFMSFFVKAA
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ANALQRFPLVNASIDGDDIIYHGYSDISIAVSTDKGLVTPVLRNVERQSFADVEQGIADY
HHHHHHCCCEECCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAKARAGKLGLDDLQGGTFTITNGGTFGSLLSTPIINPPQSAILGMHAIKERPIAENGQV
HHHHCCCCCCCHHCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCE
VIAPMMYLALSYDHRIIDGKDSVQFLVDIKNQLENPGRMLFGL
EEHHHHHHHHHCCCEEECCCCHHHHHEEHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]