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The map label for this gene is leu [H]

Identifier: 21242103

GI number: 21242103

Start: 1558786

End: 1559886

Strand: Reverse

Name: leu [H]

Synonym: XAC1350

Alternate gene names: 21242103

Gene position: 1559886-1558786 (Counterclockwise)

Preceding gene: 77748579

Following gene: 21242101

Centisome position: 30.14

GC content: 63.67

Gene sequence:

>1101_bases
ATGCTTTTCGAAACCCTCGATACCACCGGCCACGAGCAGGTGATTTTCTGTCACAACCGCGATGCCGGACTCAAGGCGAT
CATCGCCCTGCACAGCACGCGCCTTGGGCCAGCGTTGGGTGGCGTGCGGATGCGCCCCTATGCCAGTAGCGAGGCGGCAC
TCAACGACGCGCTGCGGCTGAGCCGCACCATGACCTACAAGAACGCCCTGGCCGGCCTGAATGTCGGCGGCGGCAAGGCG
GTCATCATCGGCGACCCTCGGACCGACAAGACCGAAGCGCTGTTCCGCGCCTTCGGCCGATTCGTCGACACCCTCGGCGG
GCGCTATATCACCTCCGAGGACGTCGGCACCGACGTCAACGACATGGAGCAGATCTACCTGGAAAGCGAGTACGTCACCG
GCGTGCACCAGGTGCACCGCGGCTCCGGCGACCCTGCCCCGTTTACGGCCTATGGTGCGCTGCAGGCGCTGATGGCCTCG
CTCAACCGCAAGCTCGGCCACGAAGAAGTCGGCAAGGCCAGTATCGCGATCCAGGGGCTGGGCCATATCGGCATGGAGTT
GGTGAAGCTGCTCAAGGAGCGCGGCGCAAAACTCTATGTGGCCGACCTGAATCAGGCGTTGGTCGACCGCGCCGTGGCCG
AATACGGCGCAGAAGCGGTGCGCCCGGATGAAATCCATCAGGTATCGGCCGACGTATTTGCTCCCTGCGCGCTGGAAGGC
GCCATCAACGAAGACACGCTGCCGCAACTCAAGGCCAAGATCATCTGCGGCACCGCGAACAATCAGCTTGCCAGCGCCGC
CATCGGCGAAGAGCTGCATCGGCGCGGCATTTTGTATGCGCCCGATTACGTGGTGAATGCCGGCGGGGTGATGAATGTGT
CGCTGGAGATCGACGGCTACAACCGCGAGCGCGCGATGCGGCTGATCCGCAGCATCTATCACAACCTGGAAAAGGTTTTC
GATCTGTCCACGCAGCTGGACGTTTCGCCGCAGCAGGCGGCCGATCAGATTGCCGAAGCGCGCATCGAAGCCATCAGCAA
GCTCAAACTGCCGCTGGGCCGCACCGCACCGCGCTTCCTGCACAAGCTGCGCGGCGAGTGA

Upstream 100 bases:

>100_bases
ATCGGACAGGATCTGCAAACGTCGCGTTTTTTGTTTCCCACGGCACGGGTGCATGCCCGGCTTCAGGGAAGGCACATCAC
GCAGCCCCGCGGAGCCTTCG

Downstream 100 bases:

>100_bases
AACCCTGTCCAGGCACGCTGCCTGGACAGGGACCACATGCCTCAGCCAGGCAAGGCGCCGATCAGAAATCGGCGCTGTAT
CGCAGGCTCAGCGCACGGTC

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MLFETLDTTGHEQVIFCHNRDAGLKAIIALHSTRLGPALGGVRMRPYASSEAALNDALRLSRTMTYKNALAGLNVGGGKA
VIIGDPRTDKTEALFRAFGRFVDTLGGRYITSEDVGTDVNDMEQIYLESEYVTGVHQVHRGSGDPAPFTAYGALQALMAS
LNRKLGHEEVGKASIAIQGLGHIGMELVKLLKERGAKLYVADLNQALVDRAVAEYGAEAVRPDEIHQVSADVFAPCALEG
AINEDTLPQLKAKIICGTANNQLASAAIGEELHRRGILYAPDYVVNAGGVMNVSLEIDGYNRERAMRLIRSIYHNLEKVF
DLSTQLDVSPQQAADQIAEARIEAISKLKLPLGRTAPRFLHKLRGE

Sequences:

>Translated_366_residues
MLFETLDTTGHEQVIFCHNRDAGLKAIIALHSTRLGPALGGVRMRPYASSEAALNDALRLSRTMTYKNALAGLNVGGGKA
VIIGDPRTDKTEALFRAFGRFVDTLGGRYITSEDVGTDVNDMEQIYLESEYVTGVHQVHRGSGDPAPFTAYGALQALMAS
LNRKLGHEEVGKASIAIQGLGHIGMELVKLLKERGAKLYVADLNQALVDRAVAEYGAEAVRPDEIHQVSADVFAPCALEG
AINEDTLPQLKAKIICGTANNQLASAAIGEELHRRGILYAPDYVVNAGGVMNVSLEIDGYNRERAMRLIRSIYHNLEKVF
DLSTQLDVSPQQAADQIAEARIEAISKLKLPLGRTAPRFLHKLRGE
>Mature_366_residues
MLFETLDTTGHEQVIFCHNRDAGLKAIIALHSTRLGPALGGVRMRPYASSEAALNDALRLSRTMTYKNALAGLNVGGGKA
VIIGDPRTDKTEALFRAFGRFVDTLGGRYITSEDVGTDVNDMEQIYLESEYVTGVHQVHRGSGDPAPFTAYGALQALMAS
LNRKLGHEEVGKASIAIQGLGHIGMELVKLLKERGAKLYVADLNQALVDRAVAEYGAEAVRPDEIHQVSADVFAPCALEG
AINEDTLPQLKAKIICGTANNQLASAAIGEELHRRGILYAPDYVVNAGGVMNVSLEIDGYNRERAMRLIRSIYHNLEKVF
DLSTQLDVSPQQAADQIAEARIEAISKLKLPLGRTAPRFLHKLRGE

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Drosophila melanogaster, GI45553475, Length=382, Percent_Identity=25.130890052356, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45549226, Length=369, Percent_Identity=24.9322493224932, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 39713; Mature: 39713

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFETLDTTGHEQVIFCHNRDAGLKAIIALHSTRLGPALGGVRMRPYASSEAALNDALRL
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHCCCEECCCCCCHHHHHHHHHH
SRTMTYKNALAGLNVGGGKAVIIGDPRTDKTEALFRAFGRFVDTLGGRYITSEDVGTDVN
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHH
DMEQIYLESEYVTGVHQVHRGSGDPAPFTAYGALQALMASLNRKLGHEEVGKASIAIQGL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCHHEEEHHH
GHIGMELVKLLKERGAKLYVADLNQALVDRAVAEYGAEAVRPDEIHQVSADVFAPCALEG
HHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCC
AINEDTLPQLKAKIICGTANNQLASAAIGEELHRRGILYAPDYVVNAGGVMNVSLEIDGY
CCCCCCHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEEEECCC
NRERAMRLIRSIYHNLEKVFDLSTQLDVSPQQAADQIAEARIEAISKLKLPLGRTAPRFL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
HKLRGE
HHHCCC
>Mature Secondary Structure
MLFETLDTTGHEQVIFCHNRDAGLKAIIALHSTRLGPALGGVRMRPYASSEAALNDALRL
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHCCCEECCCCCCHHHHHHHHHH
SRTMTYKNALAGLNVGGGKAVIIGDPRTDKTEALFRAFGRFVDTLGGRYITSEDVGTDVN
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHH
DMEQIYLESEYVTGVHQVHRGSGDPAPFTAYGALQALMASLNRKLGHEEVGKASIAIQGL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCHHEEEHHH
GHIGMELVKLLKERGAKLYVADLNQALVDRAVAEYGAEAVRPDEIHQVSADVFAPCALEG
HHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCC
AINEDTLPQLKAKIICGTANNQLASAAIGEELHRRGILYAPDYVVNAGGVMNVSLEIDGY
CCCCCCHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEEEECCC
NRERAMRLIRSIYHNLEKVFDLSTQLDVSPQQAADQIAEARIEAISKLKLPLGRTAPRFL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
HKLRGE
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]