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The map label for this gene is mrdA [H]

Identifier: 21241430

GI number: 21241430

Start: 779917

End: 781977

Strand: Direct

Name: mrdA [H]

Synonym: XAC0659

Alternate gene names: 21241430

Gene position: 779917-781977 (Clockwise)

Preceding gene: 21241429

Following gene: 21241431

Centisome position: 15.07

GC content: 68.27

Gene sequence:

>2061_bases
ATGCACGGGCGCCGTCATCCGAAGAATCCGCATGCGGAGGCCGAACAGTTCCGCCGCCGCGCGGTGCTGGGCTTTGCGAT
GGTGGTGATCTGCCTGGTCGGCCTGGGCGGCTGGTATTTCAAGCTGCAGGTGCTCGACCACGAGGTCTACGCCACCCGCT
CGGAGGCCAACCGCATCAAGCCCAAGCCGGTGGTGCCCGGGCGCGGCATGATCTACGACCGCACCGGCCGGCTGCTGGCC
GAGAACGTGCCGGCATTCCGTCTGGACGTGACCCCCGACAAGGTCGCCGACATGAACGCCATGCTGGACGCGTTGGGCAA
GGTGATCCCGATCGCGCCGGAGGATCTGGAGCGCTTCAACCGCGAGCGCCGCGCGCGCCGCAGCTTCCTGCCGGTGACGC
TCAAGCTGCGCATGAGCGATGAAGAGATGGCTCGCTTTGCGGTGGAGCGCTGGCGCTTCCCCGGTGTGGAGCTGGAGCCG
TATCTGACCCGGCGTTATCCCTACGGCCCGTTGTTCGCGCACATCATCGGCTATGTGGGCCGGATCGACGAAAAAGACCT
GGCGGTGCTGGGCGAGGGCAATGCTGCGCTCACCCACATGGGCAAATCCGGGCTGGAGCGCTACTACGAGCAGGATCTGC
GCGGGCAGGTCGGTTACGAGCAGGTCGAAACCAACGTGCAGGGGCGTGCCATCCGCACCGTCGGCCGGGTCGCGCCGCAG
TCGGGTGCGGATCTGCGCCTGTCGGTAGACGCCGACCTGCAGCGCGCGATGGTGGCCGCCTTCGGCGAGCACGAAGGGGC
GGCCATCGCGATGGACCCGCGCACCGGCGAGATCCTGGGCATGGTCAGCCTGCCCAGCTACGACCCCAATCTGTTCGTCA
ACGGCATCTCGCATACCGATTTCAAGATGCTCAACGACAACCCCTCGCGTCCGCAGTTCAACCGGCTGGTGCTGGGGGGC
GTGGCGCCGGGCTCCACGCTCAAGCCGCTGATCGCGTTGGCCGGCCTGGACAGCGGCCTGCGCCGGCCGGAAGACCGGGT
GCTGTCCACCGGCATGTTCTACCTGCCCGGCGTCAGTCGCGGTTGGGGCGATTCGCACCGTGGCGGCCATGGCTGGACCG
ACCTGCGCAAATCGATCGCCCAGTCGGTCAATACCTATTACTACCGCCTGGCGGTGGACATGGGCATCGAGCGCTTCGAC
CGCTACATGGGCGAGTACGGCTTTGGCCAACCCACTGGTGTGGATCTGCTGGGCGAAATCGGCGGCATCCTGCCGTCGCC
GGCCTACAAGTACAAGACGCGCAAGGAGCGCTGGTATCCCGGCGACACGGTCAACATCGCCATCGGCCAGGGCGACTGGA
AGGTGACACCGCTGCAGCTGGTGCGCGGCGTCTCGGCGATCGCCAGTGGCCTGCTGCTGCGCCCGCATCTGGTGCTGGAA
GAGCGCACCGGTTTCGATCACCCCTGGGAGCCGATGATCCAGCCGCCAGGCAAGCCGCTCAGCCAGAACCCCGCGCATCT
GCAAGTGGTGCGCGAAGGCATGATGGACACCATGCGGCCCGGCGGCACCGGCTACGCCATCACCCCTGGCGCGCCGTACC
AGATGGCGGGCAAGACCGGCACCGCGCAGGTGGTCAGCCGCAAGGGCGTGGCGGCAGTGGATCCGCGCAGCCTACCGTTG
CACCTGCGTCACCGCGGCCTGTTCGTCGGCTTCGCGCCGGCCGACAACCCGGTCATCGCGGTGGCGGTGGCGGTGGAAGG
CGGCGGTTTCGGCACCAGCTCGGCGGCGCCGATCGCACGCAAGATCTTCGATGCCTGGCTGCTGGGCAAGATGCCCGCCG
GTCTGGAGCCGTTGGACAGCGAGCTGGGCGTGACCGCCGTGGGCGTCAATCAGTTCGAGGCCGGGGTCACCGACGCGCGT
GCCGCCGGCGATGCCGCCGCTGCCACGCTGGACGAGTTGCCGGTGGTGATCGGCCAGAGCGCCGTTGCGCTGGACCCCAA
CATCCCCGCGCAACCGGTGGTGCCGGATGTGCCGGACGCGGTGCAGGAGACCGACCATTGA

Upstream 100 bases:

>100_bases
GAGCTACTGGTGGGCGCCGCTGCTGGGCATGGCCTTGTGGCCGCCGCTGTTCGTGGTGCTGGATGCGGTGCGGCTGGGCA
AGCGGGGCAAGAAATAAGCC

Downstream 100 bases:

>100_bases
GCGACATCCTGCGCTGGTTGGCGGACATGGCCGCGCGCTTTACGCGCAGCCTGGACTGGGTGCTGTGCCTGGCGCTCGGC
GGCCTGATGGCCATCGGCCT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MHGRRHPKNPHAEAEQFRRRAVLGFAMVVICLVGLGGWYFKLQVLDHEVYATRSEANRIKPKPVVPGRGMIYDRTGRLLA
ENVPAFRLDVTPDKVADMNAMLDALGKVIPIAPEDLERFNRERRARRSFLPVTLKLRMSDEEMARFAVERWRFPGVELEP
YLTRRYPYGPLFAHIIGYVGRIDEKDLAVLGEGNAALTHMGKSGLERYYEQDLRGQVGYEQVETNVQGRAIRTVGRVAPQ
SGADLRLSVDADLQRAMVAAFGEHEGAAIAMDPRTGEILGMVSLPSYDPNLFVNGISHTDFKMLNDNPSRPQFNRLVLGG
VAPGSTLKPLIALAGLDSGLRRPEDRVLSTGMFYLPGVSRGWGDSHRGGHGWTDLRKSIAQSVNTYYYRLAVDMGIERFD
RYMGEYGFGQPTGVDLLGEIGGILPSPAYKYKTRKERWYPGDTVNIAIGQGDWKVTPLQLVRGVSAIASGLLLRPHLVLE
ERTGFDHPWEPMIQPPGKPLSQNPAHLQVVREGMMDTMRPGGTGYAITPGAPYQMAGKTGTAQVVSRKGVAAVDPRSLPL
HLRHRGLFVGFAPADNPVIAVAVAVEGGGFGTSSAAPIARKIFDAWLLGKMPAGLEPLDSELGVTAVGVNQFEAGVTDAR
AAGDAAAATLDELPVVIGQSAVALDPNIPAQPVVPDVPDAVQETDH

Sequences:

>Translated_686_residues
MHGRRHPKNPHAEAEQFRRRAVLGFAMVVICLVGLGGWYFKLQVLDHEVYATRSEANRIKPKPVVPGRGMIYDRTGRLLA
ENVPAFRLDVTPDKVADMNAMLDALGKVIPIAPEDLERFNRERRARRSFLPVTLKLRMSDEEMARFAVERWRFPGVELEP
YLTRRYPYGPLFAHIIGYVGRIDEKDLAVLGEGNAALTHMGKSGLERYYEQDLRGQVGYEQVETNVQGRAIRTVGRVAPQ
SGADLRLSVDADLQRAMVAAFGEHEGAAIAMDPRTGEILGMVSLPSYDPNLFVNGISHTDFKMLNDNPSRPQFNRLVLGG
VAPGSTLKPLIALAGLDSGLRRPEDRVLSTGMFYLPGVSRGWGDSHRGGHGWTDLRKSIAQSVNTYYYRLAVDMGIERFD
RYMGEYGFGQPTGVDLLGEIGGILPSPAYKYKTRKERWYPGDTVNIAIGQGDWKVTPLQLVRGVSAIASGLLLRPHLVLE
ERTGFDHPWEPMIQPPGKPLSQNPAHLQVVREGMMDTMRPGGTGYAITPGAPYQMAGKTGTAQVVSRKGVAAVDPRSLPL
HLRHRGLFVGFAPADNPVIAVAVAVEGGGFGTSSAAPIARKIFDAWLLGKMPAGLEPLDSELGVTAVGVNQFEAGVTDAR
AAGDAAAATLDELPVVIGQSAVALDPNIPAQPVVPDVPDAVQETDH
>Mature_686_residues
MHGRRHPKNPHAEAEQFRRRAVLGFAMVVICLVGLGGWYFKLQVLDHEVYATRSEANRIKPKPVVPGRGMIYDRTGRLLA
ENVPAFRLDVTPDKVADMNAMLDALGKVIPIAPEDLERFNRERRARRSFLPVTLKLRMSDEEMARFAVERWRFPGVELEP
YLTRRYPYGPLFAHIIGYVGRIDEKDLAVLGEGNAALTHMGKSGLERYYEQDLRGQVGYEQVETNVQGRAIRTVGRVAPQ
SGADLRLSVDADLQRAMVAAFGEHEGAAIAMDPRTGEILGMVSLPSYDPNLFVNGISHTDFKMLNDNPSRPQFNRLVLGG
VAPGSTLKPLIALAGLDSGLRRPEDRVLSTGMFYLPGVSRGWGDSHRGGHGWTDLRKSIAQSVNTYYYRLAVDMGIERFD
RYMGEYGFGQPTGVDLLGEIGGILPSPAYKYKTRKERWYPGDTVNIAIGQGDWKVTPLQLVRGVSAIASGLLLRPHLVLE
ERTGFDHPWEPMIQPPGKPLSQNPAHLQVVREGMMDTMRPGGTGYAITPGAPYQMAGKTGTAQVVSRKGVAAVDPRSLPL
HLRHRGLFVGFAPADNPVIAVAVAVEGGGFGTSSAAPIARKIFDAWLLGKMPAGLEPLDSELGVTAVGVNQFEAGVTDAR
AAGDAAAATLDELPVVIGQSAVALDPNIPAQPVVPDVPDAVQETDH

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=606, Percent_Identity=38.4488448844884, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1786272, Length=614, Percent_Identity=27.8501628664495, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 74772; Mature: 74772

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGRRHPKNPHAEAEQFRRRAVLGFAMVVICLVGLGGWYFKLQVLDHEVYATRSEANRIK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCC
PKPVVPGRGMIYDRTGRLLAENVPAFRLDVTPDKVADMNAMLDALGKVIPIAPEDLERFN
CCCCCCCCCEEEECCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RERRARRSFLPVTLKLRMSDEEMARFAVERWRFPGVELEPYLTRRYPYGPLFAHIIGYVG
HHHHHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHH
RIDEKDLAVLGEGNAALTHMGKSGLERYYEQDLRGQVGYEQVETNVQGRAIRTVGRVAPQ
CCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCCC
SGADLRLSVDADLQRAMVAAFGEHEGAAIAMDPRTGEILGMVSLPSYDPNLFVNGISHTD
CCCCEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCEEEECCCCCC
FKMLNDNPSRPQFNRLVLGGVAPGSTLKPLIALAGLDSGLRRPEDRVLSTGMFYLPGVSR
EEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHCCCHHHHHHCCEEECCCCCC
GWGDSHRGGHGWTDLRKSIAQSVNTYYYRLAVDMGIERFDRYMGEYGFGQPTGVDLLGEI
CCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GGILPSPAYKYKTRKERWYPGDTVNIAIGQGDWKVTPLQLVRGVSAIASGLLLRPHLVLE
CCCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCEECHHHHHHHHHHHHHCHHCCCEEEEE
ERTGFDHPWEPMIQPPGKPLSQNPAHLQVVREGMMDTMRPGGTGYAITPGAPYQMAGKTG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCC
TAQVVSRKGVAAVDPRSLPLHLRHRGLFVGFAPADNPVIAVAVAVEGGGFGTSSAAPIAR
HHHHHHCCCCEEECCCCCCEEEEECEEEEEECCCCCCEEEEEEEEECCCCCCCCCCHHHH
KIFDAWLLGKMPAGLEPLDSELGVTAVGVNQFEAGVTDARAAGDAAAATLDELPVVIGQS
HHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHCCCCHHHHCCCHHHHHHHHCCHHHCCC
AVALDPNIPAQPVVPDVPDAVQETDH
EEEECCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MHGRRHPKNPHAEAEQFRRRAVLGFAMVVICLVGLGGWYFKLQVLDHEVYATRSEANRIK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCC
PKPVVPGRGMIYDRTGRLLAENVPAFRLDVTPDKVADMNAMLDALGKVIPIAPEDLERFN
CCCCCCCCCEEEECCCCHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RERRARRSFLPVTLKLRMSDEEMARFAVERWRFPGVELEPYLTRRYPYGPLFAHIIGYVG
HHHHHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHH
RIDEKDLAVLGEGNAALTHMGKSGLERYYEQDLRGQVGYEQVETNVQGRAIRTVGRVAPQ
CCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCCC
SGADLRLSVDADLQRAMVAAFGEHEGAAIAMDPRTGEILGMVSLPSYDPNLFVNGISHTD
CCCCEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCEEEECCCCCC
FKMLNDNPSRPQFNRLVLGGVAPGSTLKPLIALAGLDSGLRRPEDRVLSTGMFYLPGVSR
EEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHCCCHHHHHHCCEEECCCCCC
GWGDSHRGGHGWTDLRKSIAQSVNTYYYRLAVDMGIERFDRYMGEYGFGQPTGVDLLGEI
CCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GGILPSPAYKYKTRKERWYPGDTVNIAIGQGDWKVTPLQLVRGVSAIASGLLLRPHLVLE
CCCCCCCCHHHCCCCCCCCCCCEEEEEEECCCCEECHHHHHHHHHHHHHCHHCCCEEEEE
ERTGFDHPWEPMIQPPGKPLSQNPAHLQVVREGMMDTMRPGGTGYAITPGAPYQMAGKTG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCC
TAQVVSRKGVAAVDPRSLPLHLRHRGLFVGFAPADNPVIAVAVAVEGGGFGTSSAAPIAR
HHHHHHCCCCEEECCCCCCEEEEECEEEEEECCCCCCEEEEEEEEECCCCCCCCCCHHHH
KIFDAWLLGKMPAGLEPLDSELGVTAVGVNQFEAGVTDARAAGDAAAATLDELPVVIGQS
HHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHCCCCHHHHCCCHHHHHHHHCCHHHCCC
AVALDPNIPAQPVVPDVPDAVQETDH
EEEECCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]