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The map label for this gene is pcaF [H]

Identifier: 21241140

GI number: 21241140

Start: 436568

End: 437776

Strand: Direct

Name: pcaF [H]

Synonym: XAC0366

Alternate gene names: 21241140

Gene position: 436568-437776 (Clockwise)

Preceding gene: 21241139

Following gene: 21241141

Centisome position: 8.44

GC content: 69.31

Gene sequence:

>1209_bases
ATGAATGAGGTCTATCTGATCGATGGCATTCGCACGCCGATCGGCCGTTACGCTGGCGCCTTGTCCAGCGTACGCGCCGA
CGACCTGGGCGCAGTGCCGATCGCCGCATTGCTGGCGCGGCAATCGCAGCTGGAGCCGGCTGCAATCGACGATGTATGCC
TGGGCTGCGCCAACCAGGCCGGCGAAGACAATCGCAATGTCGCGCGTATGAGCCTGTTGCTGGCCGGCTTGCCGCCTTCG
GTGCCGGGCAGCACGCTCAATCGTCTGTGCGGCTCCGGGTTGGACGCGATCGGCACGGTGGCGCGCGGCATTCGTGCCGG
CGAACTGGATCTTGCCATTGCCGGTGGGGTCGAATCGATGTCGCGCGCGCCGTGGGTGATGGGCAAGGCCGACAGCGCCT
TCGCCCGCACCCAGCAACTGGAAGACACCACCATGGGCTGGCGCTTCATCAATCCGCGCCTGCAGGCCGTCTATGGCACC
GAATTGATGGGTGAGACGGCAGAAAACGTGGCGCAGCGGTACGCCATCGCGCGCGAGGACCAGGACGCGTTCGCACTGCG
CAGCCAGCAACGCGTGGCGGCGGCGCAGGCAGCCGGTTTCTTCGATGCGGAAATCGCCCCGGTGGCCGTGGCCGGGCGCA
AGGGCGGCGATGCCTCCACCGTCGAACACGACGAAGCGCCGCGGCCCAATACCACCGCCGAGATGCTGGCCAAGCTCAAG
CCGGTGTTCCGCCAGCCCGGCACCGTCACGGCCGGCAACGCCTCCGGTCTCAACGATGGCGCCGCCGCCTTGCTGCTGGC
CTCTGCGCAGGCCGTGCAGACGCATCGGCTGACGCCGCGCGCGCGGGTATTGGGGTTCGCCTCGGCGGGCGTGGAGCCGG
CGTACATGGGCATCGGCCCGGTGCCGGCCGCCCAGCGCCTGATGGCGCGCTTGCACCTGCGCATCGCGCAGTTCGACGCG
ATCGAACTCAATGAAGCCTTCGCTGCGCAGGCGCTGGCCTGCACGCGCGCATTCGGCCTGGCCGACGATGCCGCACACGT
CAATGCCAACGGTGGCGCCATTGCGCTCGGGCATCCGCTCGGCATGAGCGGCGCGCGGCTGGCGCTGACCTTGCTGCGGC
AGCTGGAGGCGGGCAACGGGCGATTCGGGCTGGCCAGCATGTGCATCGGGGTGGGGCAGGGCGTGGCGCTGGCGATCGAG
CGCCTGTGA

Upstream 100 bases:

>100_bases
CACACACCACGCCGCCCCGTGCCGCCGAGCTGGACGCCTTGCGCGCGCTGCAGGCACGCACCGATGCAGCGCATGGTGCC
CAGGCAACGGGAGCCCAGGC

Downstream 100 bases:

>100_bases
ACCTGCTGTTGCAACCTCCCTCGTCCTTCCCTCGCACCCATGTCGTTCTAGTCGTCTTGCTGCCGACGGAGATTTCAAGA
TGCGCGATCCCACCACCGAC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNEVYLIDGIRTPIGRYAGALSSVRADDLGAVPIAALLARQSQLEPAAIDDVCLGCANQAGEDNRNVARMSLLLAGLPPS
VPGSTLNRLCGSGLDAIGTVARGIRAGELDLAIAGGVESMSRAPWVMGKADSAFARTQQLEDTTMGWRFINPRLQAVYGT
ELMGETAENVAQRYAIAREDQDAFALRSQQRVAAAQAAGFFDAEIAPVAVAGRKGGDASTVEHDEAPRPNTTAEMLAKLK
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQTHRLTPRARVLGFASAGVEPAYMGIGPVPAAQRLMARLHLRIAQFDA
IELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPLGMSGARLALTLLRQLEAGNGRFGLASMCIGVGQGVALAIE
RL

Sequences:

>Translated_402_residues
MNEVYLIDGIRTPIGRYAGALSSVRADDLGAVPIAALLARQSQLEPAAIDDVCLGCANQAGEDNRNVARMSLLLAGLPPS
VPGSTLNRLCGSGLDAIGTVARGIRAGELDLAIAGGVESMSRAPWVMGKADSAFARTQQLEDTTMGWRFINPRLQAVYGT
ELMGETAENVAQRYAIAREDQDAFALRSQQRVAAAQAAGFFDAEIAPVAVAGRKGGDASTVEHDEAPRPNTTAEMLAKLK
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQTHRLTPRARVLGFASAGVEPAYMGIGPVPAAQRLMARLHLRIAQFDA
IELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPLGMSGARLALTLLRQLEAGNGRFGLASMCIGVGQGVALAIE
RL
>Mature_402_residues
MNEVYLIDGIRTPIGRYAGALSSVRADDLGAVPIAALLARQSQLEPAAIDDVCLGCANQAGEDNRNVARMSLLLAGLPPS
VPGSTLNRLCGSGLDAIGTVARGIRAGELDLAIAGGVESMSRAPWVMGKADSAFARTQQLEDTTMGWRFINPRLQAVYGT
ELMGETAENVAQRYAIAREDQDAFALRSQQRVAAAQAAGFFDAEIAPVAVAGRKGGDASTVEHDEAPRPNTTAEMLAKLK
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQTHRLTPRARVLGFASAGVEPAYMGIGPVPAAQRLMARLHLRIAQFDA
IELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPLGMSGARLALTLLRQLEAGNGRFGLASMCIGVGQGVALAIE
RL

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=400, Percent_Identity=41.5, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI148539872, Length=403, Percent_Identity=40.1985111662531, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4501853, Length=404, Percent_Identity=41.0891089108911, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=38.7096774193548, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=31.002331002331, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI194353979, Length=403, Percent_Identity=32.258064516129, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=63.18407960199, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=46.1538461538462, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.6384039900249, Blast_Score=297, Evalue=7e-82,
Organism=Escherichia coli, GI48994986, Length=411, Percent_Identity=43.7956204379562, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1788683, Length=415, Percent_Identity=32.7710843373494, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=40.8521303258145, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=35.5, Blast_Score=227, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.7468982630273, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=429, Percent_Identity=29.3706293706294, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=410, Percent_Identity=30.2439024390244, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=37.1007371007371, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6322031, Length=329, Percent_Identity=39.8176291793313, Blast_Score=225, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=45.5223880597015, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=39.5, Blast_Score=257, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=36.9077306733167, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=31.3084112149533, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41652; Mature: 41652

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVYLIDGIRTPIGRYAGALSSVRADDLGAVPIAALLARQSQLEPAAIDDVCLGCANQA
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
GEDNRNVARMSLLLAGLPPSVPGSTLNRLCGSGLDAIGTVARGIRAGELDLAIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCHHHH
SRAPWVMGKADSAFARTQQLEDTTMGWRFINPRLQAVYGTELMGETAENVAQRYAIARED
HCCCEEECCCCHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QDAFALRSQQRVAAAQAAGFFDAEIAPVAVAGRKGGDASTVEHDEAPRPNTTAEMLAKLK
CHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQTHRLTPRARVLGFASAGVEPAYMGIGP
HHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCCCCCCC
VPAAQRLMARLHLRIAQFDAIELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPL
CHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCC
GMSGARLALTLLRQLEAGNGRFGLASMCIGVGQGVALAIERL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHEECC
>Mature Secondary Structure
MNEVYLIDGIRTPIGRYAGALSSVRADDLGAVPIAALLARQSQLEPAAIDDVCLGCANQA
CCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
GEDNRNVARMSLLLAGLPPSVPGSTLNRLCGSGLDAIGTVARGIRAGELDLAIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCHHHH
SRAPWVMGKADSAFARTQQLEDTTMGWRFINPRLQAVYGTELMGETAENVAQRYAIARED
HCCCEEECCCCHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QDAFALRSQQRVAAAQAAGFFDAEIAPVAVAGRKGGDASTVEHDEAPRPNTTAEMLAKLK
CHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHH
PVFRQPGTVTAGNASGLNDGAAALLLASAQAVQTHRLTPRARVLGFASAGVEPAYMGIGP
HHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCCCCCCC
VPAAQRLMARLHLRIAQFDAIELNEAFAAQALACTRAFGLADDAAHVNANGGAIALGHPL
CHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCC
GMSGARLALTLLRQLEAGNGRFGLASMCIGVGQGVALAIERL
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]