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The map label for this gene is vanK [H]

Identifier: 21241123

GI number: 21241123

Start: 416057

End: 417367

Strand: Direct

Name: vanK [H]

Synonym: XAC0349

Alternate gene names: 21241123

Gene position: 416057-417367 (Clockwise)

Preceding gene: 21241121

Following gene: 21241124

Centisome position: 8.04

GC content: 66.21

Gene sequence:

>1311_bases
ATGCGTCAGCACACTGCATCGTTTGGGCACTTCGTGCTGGAGCGCATGACACCGTTTCAGTGGAGGGCCGTGGCGGTGTG
CGTGTTGCTGACGATGCTCGACGGCTTCGATGTGATGGCGATGGCGTTCACCGCACCGCACGTGTCGGCGGATTGGCAGC
TGTCGGGCAAGCTGCTGGGCATGCTGTTCAGCGCGGGCCTGATCGGCATGGCGCTTGGCGCACTGGGGTTGGCGCCGTTG
GCAGACCGCATCGGCCGTCGCGCGCTGACGCTGGCATGTCTGGCGATCCTGACCATCGGCATGGGCATGTCCGCACTGGC
CAGCACTGCATGGCAGCTCGGGGCGCTGCGGCTCCTGACCGGTCTGGGGATCGGCGGTATGTTGGCCTGCGTTGCGGTCA
CGGCAGGGGAGTTTTCCAGCCCCCGTTGGCGCAACACTGCCATCGTGCTGCAGGTGACCGGCTACCCGGTCGGTGCCACG
CTGGGTGGCATGATTGCCGAACTGTTGATGCAGCAATGGTCGTGGCCCGCGGTGTTCGTCCTCGGGGCGGTGGCATCGTT
GCTGTGTGTGCCGTTGGTGCTTGCATTCCTGCCCGAGTCGCTGGAGTTCCTGATCATGCGCCGGCCACCTGATGCCCTCG
CGCGCTTCAACGCCGTGCTGGCCCGGATGGGGCTGCCGCCGCACGACGCATTGCCGCCGCTACCGCCGCAGTCTGCCACG
CAGCCACAAGGCTATTCGGCCCTGTTTGACGTTGCCATGCGGCGGCACACGCTGCTGATCGCGCTTGGCTTTTTCTCGCT
GATGTTTGCGTTTTACTTCGTGATCAGCTGGACACCGAAGCTGCTGGTGACCGCTGGCTCGTCGGCCACGGAGGGCGTCA
CGGGCGGGGTGCTGTTGAATCTTGGAGGCATCTGCGGCGGCTTGCTGTTCAGTGGGTTGGCATCGTGCGTGCGGCTGTCG
CGCCTGACGGTCGGATCCCTGTTGCTGATGGGCTTGGCGATGCTGGCCTCCGGCCTGTGCAGTACGCAGTTGAGCTGGGC
GCTCGCCGTCGCCTTGCTCACCGGGGCAGCGATGTTCGCTGCGGTGGGCGGCATGTACGCCATTGTGCCGATCGTCTTCA
ACGCTGCGGTGCGCTCGACTGCGCTGGGCTGGGCGATCGGGGTGGGGCGCTTCGGTGCGATTTTGTCGCCCTTCTGTGCC
GGGGCGTTGCTGGACGCCGGATGGTCGCCGGCCATGTTGTATCTGGCCTGCAGCGTGCCGTTGCTGTTCGCCGTCGTCGC
CGTGCTGGGGATGCGGCTGCCCAACCGCTGA

Upstream 100 bases:

>100_bases
GCATCGCTGCCCCGCCGCGCAGCCGGCCTTGCACACTGCGCCGTTCAACGCGACTGCAGCGCCTTCGGGCCTATCCTTTC
TCGCACTACAGGGCATGCCA

Downstream 100 bases:

>100_bases
GCGGCCCGCCCACAGCCGCGTCCGGCGTCACCTCCAGGCCTTCCGTCGATCCGGTCGCGCGCCAAAGAAATCGTGCTGTG
TGCCGATAACACCGCAGACC

Product: MFS transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRQHTASFGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMAMAFTAPHVSADWQLSGKLLGMLFSAGLIGMALGALGLAPL
ADRIGRRALTLACLAILTIGMGMSALASTAWQLGALRLLTGLGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGAT
LGGMIAELLMQQWSWPAVFVLGAVASLLCVPLVLAFLPESLEFLIMRRPPDALARFNAVLARMGLPPHDALPPLPPQSAT
QPQGYSALFDVAMRRHTLLIALGFFSLMFAFYFVISWTPKLLVTAGSSATEGVTGGVLLNLGGICGGLLFSGLASCVRLS
RLTVGSLLLMGLAMLASGLCSTQLSWALAVALLTGAAMFAAVGGMYAIVPIVFNAAVRSTALGWAIGVGRFGAILSPFCA
GALLDAGWSPAMLYLACSVPLLFAVVAVLGMRLPNR

Sequences:

>Translated_436_residues
MRQHTASFGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMAMAFTAPHVSADWQLSGKLLGMLFSAGLIGMALGALGLAPL
ADRIGRRALTLACLAILTIGMGMSALASTAWQLGALRLLTGLGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGAT
LGGMIAELLMQQWSWPAVFVLGAVASLLCVPLVLAFLPESLEFLIMRRPPDALARFNAVLARMGLPPHDALPPLPPQSAT
QPQGYSALFDVAMRRHTLLIALGFFSLMFAFYFVISWTPKLLVTAGSSATEGVTGGVLLNLGGICGGLLFSGLASCVRLS
RLTVGSLLLMGLAMLASGLCSTQLSWALAVALLTGAAMFAAVGGMYAIVPIVFNAAVRSTALGWAIGVGRFGAILSPFCA
GALLDAGWSPAMLYLACSVPLLFAVVAVLGMRLPNR
>Mature_436_residues
MRQHTASFGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMAMAFTAPHVSADWQLSGKLLGMLFSAGLIGMALGALGLAPL
ADRIGRRALTLACLAILTIGMGMSALASTAWQLGALRLLTGLGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGAT
LGGMIAELLMQQWSWPAVFVLGAVASLLCVPLVLAFLPESLEFLIMRRPPDALARFNAVLARMGLPPHDALPPLPPQSAT
QPQGYSALFDVAMRRHTLLIALGFFSLMFAFYFVISWTPKLLVTAGSSATEGVTGGVLLNLGGICGGLLFSGLASCVRLS
RLTVGSLLLMGLAMLASGLCSTQLSWALAVALLTGAAMFAAVGGMYAIVPIVFNAAVRSTALGWAIGVGRFGAILSPFCA
GALLDAGWSPAMLYLACSVPLLFAVVAVLGMRLPNR

Specific function: Probable uptake of benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=412, Percent_Identity=25.7281553398058, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081723, Length=392, Percent_Identity=30.6122448979592, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI87082404, Length=215, Percent_Identity=25.1162790697674, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082159, Length=386, Percent_Identity=24.3523316062176, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082231, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788074, Length=350, Percent_Identity=24, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45588; Mature: 45588

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHTASFGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMAMAFTAPHVSADWQLSGKLLG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHH
MLFSAGLIGMALGALGLAPLADRIGRRALTLACLAILTIGMGMSALASTAWQLGALRLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GLGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGATLGGMIAELLMQQWSWPAVFV
HCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHH
LGAVASLLCVPLVLAFLPESLEFLIMRRPPDALARFNAVLARMGLPPHDALPPLPPQSAT
HHHHHHHHHHHHHHHHHCHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
QPQGYSALFDVAMRRHTLLIALGFFSLMFAFYFVISWTPKLLVTAGSSATEGVTGGVLLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHH
LGGICGGLLFSGLASCVRLSRLTVGSLLLMGLAMLASGLCSTQLSWALAVALLTGAAMFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGGMYAIVPIVFNAAVRSTALGWAIGVGRFGAILSPFCAGALLDAGWSPAMLYLACSVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLFAVVAVLGMRLPNR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRQHTASFGHFVLERMTPFQWRAVAVCVLLTMLDGFDVMAMAFTAPHVSADWQLSGKLLG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHH
MLFSAGLIGMALGALGLAPLADRIGRRALTLACLAILTIGMGMSALASTAWQLGALRLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GLGIGGMLACVAVTAGEFSSPRWRNTAIVLQVTGYPVGATLGGMIAELLMQQWSWPAVFV
HCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHH
LGAVASLLCVPLVLAFLPESLEFLIMRRPPDALARFNAVLARMGLPPHDALPPLPPQSAT
HHHHHHHHHHHHHHHHHCHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
QPQGYSALFDVAMRRHTLLIALGFFSLMFAFYFVISWTPKLLVTAGSSATEGVTGGVLLN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHH
LGGICGGLLFSGLASCVRLSRLTVGSLLLMGLAMLASGLCSTQLSWALAVALLTGAAMFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGGMYAIVPIVFNAAVRSTALGWAIGVGRFGAILSPFCAGALLDAGWSPAMLYLACSVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLFAVVAVLGMRLPNR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9294456 [H]