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The map label for this gene is ntrC [H]

Identifier: 21240982

GI number: 21240982

Start: 255678

End: 257105

Strand: Direct

Name: ntrC [H]

Synonym: XAC0208

Alternate gene names: 21240982

Gene position: 255678-257105 (Clockwise)

Preceding gene: 21240981

Following gene: 21240983

Centisome position: 4.94

GC content: 68.77

Gene sequence:

>1428_bases
ATGGCTGAGACCGCCGCCGTATCCCACATCTGGGTGGTCGACGACGACCGGTCGGTCCGTTTCGTGCTGTCCACCGCCTT
GCGCGATGCCGGCTACGCCGTGGACGGCTTCGACAGCGCCGCTGCCGCGCTGCAGGCGCTCGGCATGCGGCCCACGCCGG
ATTTGCTGTTCACCGACGTGCGCATGCCCGGCGAAGACGGCCTGACCCTGCTCGACAAACTCAAATCCAAACACCCGCAC
TTGCCGGTGATCGTGATGTCGGCCTATACCGACGTTGCCAGCACCGCCGGTGCCTTCCGCGGCGGCGCGCACGAGTTCCT
GTCCAAGCCGTTCGACCTGGACGACGCGGTGGCACTGGCTGCGCGCGCGCTGCCGGACGCCGATGCCGGCGTCGAAGAAA
TCGTCGCCACGCGGCTGGCCGAGGGCTCGGCCTCGTTGATCGGCGACACGCCCGCGATGCAGGCCTTGTTCCGCGCGATC
GGACGCCTGGCGCAGGCGCCGCTGTCGGTGCTGATCAATGGCGAAACCGGTACCGGCAAGGAGTTGGTCGCGCGCGCGTT
GCACAACGAATCCCCGCGCGCGCGCAAACCCTTCGTCGCGCTCAACACCGCCGCCATCCCGGCCGAATTGCTGGAAAGCG
AGTTGTTCGGCCACGAAACCGGCGCGTTCACCGGGGCGACCAAACGGCATATCGGCCGCTTCGAACAGGCCGATGGCGGC
ACGCTGTTTCTGGACGAAATCGGCGACATGCCGTTGCCGCTGCAGACCCGCCTGCTGCGCGTGTTGGCAGAGAACGAATT
CTTCCGCGTTGGTGGGCGCGAATTGATCCGCGTCGATGTGCGCGTGATCGCCGCCACCCACCAGGACCTGGAGGCGCTGG
TCGAACAAGGCCGCTTCCGCGCCGATCTGCTGCACCGCCTGGATGTGGTGCGCCTGCAATTGCCGCCGCTGCGCGAACGG
CGCGGCGACATCGCGCAACTGGCCGAAAATTTCCTGGCCATGGCCGGGCGCAAACTCGACATGCTGCCCAAGCGGCTGTC
GTCGGCCGCGCTGGAAGACCTGCGCCAGTACGACTGGCCCGGCAACGTGCGCGAACTGGAAAACGTGTGCTGGCGCCTGG
CGGCGCTGGCGACCTCGGACATCATCGACGTGGTCGACGTGGACGCCGCACTGGCGCGCGGCGGCCGTCGTCACCGCAGC
GGTCGCAGCGATGGGCAGTGGGACGACATGCTTTCCAGCTGGGCCGCGCAACGCCTGAGCGAAGGTGCCCAGGGCCTGCA
CGCCGAAGCGCGCGAGCGCCTGGACAGGACCTTGCTCGAAGCGGCCTTGCAACTCACCCAGGGCCGCCGCGCCGAAGCTG
CCGCCCGCCTGGGCCTGGGCCGCAACACGGTCACCCGCAAACTCGGCCCGGGGCGCAAACGTCGCTGA

Upstream 100 bases:

>100_bases
GAACACCACGGCACGCTGACCTATCGCTCGCGTCCGGGCCATACCGTTTTCACCCTGCTGCTGCCCGAGCTGGCCGGCGA
TCACGACAAGGAGCATCTGC

Downstream 100 bases:

>100_bases
TTTGGGTCTAACGCCCCTCTCCCATGAGAGAGGGGTTGGGGTGAGGGTGCCTGTGCCTGTGCCGATGCAAGCGCGACTGC
ACGCGCACCGATCCAACGAG

Product: two-component system regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAETAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALGMRPTPDLLFTDVRMPGEDGLTLLDKLKSKHPH
LPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALAARALPDADAGVEEIVATRLAEGSASLIGDTPAMQALFRAI
GRLAQAPLSVLINGETGTGKELVARALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVEQGRFRADLLHRLDVVRLQLPPLRER
RGDIAQLAENFLAMAGRKLDMLPKRLSSAALEDLRQYDWPGNVRELENVCWRLAALATSDIIDVVDVDAALARGGRRHRS
GRSDGQWDDMLSSWAAQRLSEGAQGLHAEARERLDRTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKRR

Sequences:

>Translated_475_residues
MAETAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALGMRPTPDLLFTDVRMPGEDGLTLLDKLKSKHPH
LPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALAARALPDADAGVEEIVATRLAEGSASLIGDTPAMQALFRAI
GRLAQAPLSVLINGETGTGKELVARALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVEQGRFRADLLHRLDVVRLQLPPLRER
RGDIAQLAENFLAMAGRKLDMLPKRLSSAALEDLRQYDWPGNVRELENVCWRLAALATSDIIDVVDVDAALARGGRRHRS
GRSDGQWDDMLSSWAAQRLSEGAQGLHAEARERLDRTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKRR
>Mature_474_residues
AETAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALGMRPTPDLLFTDVRMPGEDGLTLLDKLKSKHPHL
PVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALAARALPDADAGVEEIVATRLAEGSASLIGDTPAMQALFRAIG
RLAQAPLSVLINGETGTGKELVARALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGGT
LFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVEQGRFRADLLHRLDVVRLQLPPLRERR
GDIAQLAENFLAMAGRKLDMLPKRLSSAALEDLRQYDWPGNVRELENVCWRLAALATSDIIDVVDVDAALARGGRRHRSG
RSDGQWDDMLSSWAAQRLSEGAQGLHAEARERLDRTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKRR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=460, Percent_Identity=52.6086956521739, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=42.7645788336933, Blast_Score=321, Evalue=6e-89,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=38.8888888888889, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788905, Length=469, Percent_Identity=37.953091684435, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=46.7889908256881, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789087, Length=246, Percent_Identity=50.4065040650407, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=41.2307692307692, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=52.6530612244898, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=52.7896995708155, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=41.4925373134328, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=43.2773109243698, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI87081858, Length=337, Percent_Identity=34.4213649851632, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789828, Length=283, Percent_Identity=33.9222614840989, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787375, Length=109, Percent_Identity=30.2752293577982, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51700; Mature: 51568

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALGMRPTPDLLFTDV
CCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEC
RMPGEDGLTLLDKLKSKHPHLPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALA
CCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHH
ARALPDADAGVEEIVATRLAEGSASLIGDTPAMQALFRAIGRLAQAPLSVLINGETGTGK
HHHCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
ELVARALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
HHHHHHHHCCCCCCCCCEEEECCHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCC
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVEQGRFR
EEEHHHCCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ADLLHRLDVVRLQLPPLRERRGDIAQLAENFLAMAGRKLDMLPKRLSSAALEDLRQYDWP
HHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GNVRELENVCWRLAALATSDIIDVVDVDAALARGGRRHRSGRSDGQWDDMLSSWAAQRLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHH
EGAQGLHAEARERLDRTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKRR
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
AETAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALGMRPTPDLLFTDV
CCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEC
RMPGEDGLTLLDKLKSKHPHLPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALA
CCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHH
ARALPDADAGVEEIVATRLAEGSASLIGDTPAMQALFRAIGRLAQAPLSVLINGETGTGK
HHHCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
ELVARALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
HHHHHHHHCCCCCCCCCEEEECCHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCC
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVEQGRFR
EEEHHHCCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ADLLHRLDVVRLQLPPLRERRGDIAQLAENFLAMAGRKLDMLPKRLSSAALEDLRQYDWP
HHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GNVRELENVCWRLAALATSDIIDVVDVDAALARGGRRHRSGRSDGQWDDMLSSWAAQRLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHH
EGAQGLHAEARERLDRTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKRR
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]