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The map label for this gene is ntrB [H]

Identifier: 21240981

GI number: 21240981

Start: 254630

End: 255685

Strand: Direct

Name: ntrB [H]

Synonym: XAC0207

Alternate gene names: 21240981

Gene position: 254630-255685 (Clockwise)

Preceding gene: 21240980

Following gene: 21240982

Centisome position: 4.92

GC content: 69.89

Gene sequence:

>1056_bases
ATGGTGACGTCCTCTTCCATGCCATCGCTGGACAGCCTGGGCACACCGGTCGCCTGGGCCGACCGCGAGGGCCGCGTGCT
GGGCGTCAACCCGGCGTTTGCCCGCTGGCTCGGGGTCAGTGCACGGCGGTTGGTCGAACAGCCCCTGGCCGCGCTGGAAG
TGCAGGGCGATGCGCTGGCGCGCTTCCTGCTCAACGACGAGCGCGACATATTGCGTCTGCACCGGCTGGCGCTGGGCCTG
CCCAACGACGCGCCGCGCTTTGCCGAAGGCTGGCTGTCGCGGCTGGACGATGGCGGCTGGCTGCTGGAAACCCACCCGGT
CGACGAATTCCCCGCGCTCGATCCCACCCACGCCCTGCCCAACGCCTTGAGCGCTGCGCTCAAAGGGTTGGCGCACGAAC
TGCGCAATCCGCTGGCCGGGCTGAAGGGCGCCGCGCAGCTGCTGGCACGCCGCGCGGCCGGGCGCGACGAAGACGAACGC
GAGCTGATCGAGCTGATCGGCACCGAGATCGAGCGCCTCAATACCTTGCTCGAACGGCTGCTGTCGCCCACCCCGGCGCG
CCCGCACGACCGCCTCAACATCCATGTGGTGCTCGAACGGGTGTTGCGCCTGGCCGAAGCCGAGGGCGGCTGGTCGGTGC
AGGTGCAGCGCGACTACGACCCCAGCATTCCCGACATTCTGGGCGATGGCGACCGCCTGACCCAGGCGGTCTGGAACCTG
GTGCGTAACGCCTTCCAGGCCGGCGCCACGCGCGTGACGCTGCGCACCCGCGTCGAGCATGGCCAGCGCATCCGCGACCG
CGTGCATGCGATGTCGCTGCGGCTGGAAATCATCGACGACGGCGGCGGCGTGCCGGAAGAACTGGCCGAGCACCTGTTTC
TGCCACTGGTCAGCGGCCGCGCCGAAGGCAGCGGGCTGGGCCTGGCGCTGGCCCAGCAGGTCTCGCGCGAACACCACGGC
ACGCTGACCTATCGCTCGCGTCCGGGCCATACCGTTTTCACCCTGCTGCTGCCCGAGCTGGCCGGCGATCACGACAAGGA
GCATCTGCATGGCTGA

Upstream 100 bases:

>100_bases
GCGGTGCGCCCCGCCGCCGTCTGGCCAGCATTGTTTCAACGCACGCTTCGCGTTGCGCGCCATCCGCGCATTCCCATTCT
GTTTGCACTAAATTGGTGCA

Downstream 100 bases:

>100_bases
GACCGCCGCCGTATCCCACATCTGGGTGGTCGACGACGACCGGTCGGTCCGTTTCGTGCTGTCCACCGCCTTGCGCGATG
CCGGCTACGCCGTGGACGGC

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MVTSSSMPSLDSLGTPVAWADREGRVLGVNPAFARWLGVSARRLVEQPLAALEVQGDALARFLLNDERDILRLHRLALGL
PNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALPNALSAALKGLAHELRNPLAGLKGAAQLLARRAAGRDEDER
ELIELIGTEIERLNTLLERLLSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGRAEGSGLGLALAQQVSREHHG
TLTYRSRPGHTVFTLLLPELAGDHDKEHLHG

Sequences:

>Translated_351_residues
MVTSSSMPSLDSLGTPVAWADREGRVLGVNPAFARWLGVSARRLVEQPLAALEVQGDALARFLLNDERDILRLHRLALGL
PNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALPNALSAALKGLAHELRNPLAGLKGAAQLLARRAAGRDEDER
ELIELIGTEIERLNTLLERLLSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGRAEGSGLGLALAQQVSREHHG
TLTYRSRPGHTVFTLLLPELAGDHDKEHLHG
>Mature_351_residues
MVTSSSMPSLDSLGTPVAWADREGRVLGVNPAFARWLGVSARRLVEQPLAALEVQGDALARFLLNDERDILRLHRLALGL
PNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALPNALSAALKGLAHELRNPLAGLKGAAQLLARRAAGRDEDER
ELIELIGTEIERLNTLLERLLSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGRAEGSGLGLALAQQVSREHHG
TLTYRSRPGHTVFTLLLPELAGDHDKEHLHG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=350, Percent_Identity=36, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=31.9444444444444, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=30.3571428571429, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789808, Length=203, Percent_Identity=26.1083743842365, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=378, Percent_Identity=24.0740740740741, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38631; Mature: 38631

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSSSMPSLDSLGTPVAWADREGRVLGVNPAFARWLGVSARRLVEQPLAALEVQGDALA
CCCCCCCCCHHHCCCCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RFLLNDERDILRLHRLALGLPNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALP
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHH
NALSAALKGLAHELRNPLAGLKGAAQLLARRAAGRDEDERELIELIGTEIERLNTLLERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHH
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGR
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCC
AEGSGLGLALAQQVSREHHGTLTYRSRPGHTVFTLLLPELAGDHDKEHLHG
CCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MVTSSSMPSLDSLGTPVAWADREGRVLGVNPAFARWLGVSARRLVEQPLAALEVQGDALA
CCCCCCCCCHHHCCCCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RFLLNDERDILRLHRLALGLPNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALP
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHH
NALSAALKGLAHELRNPLAGLKGAAQLLARRAAGRDEDERELIELIGTEIERLNTLLERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHH
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGR
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCC
AEGSGLGLALAQQVSREHHGTLTYRSRPGHTVFTLLLPELAGDHDKEHLHG
CCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]