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The map label for this gene is lcfB [H]

Identifier: 21226716

GI number: 21226716

Start: 745291

End: 746778

Strand: Reverse

Name: lcfB [H]

Synonym: MM_0614

Alternate gene names: 21226716

Gene position: 746778-745291 (Counterclockwise)

Preceding gene: 21226717

Following gene: 21226715

Centisome position: 18.23

GC content: 46.24

Gene sequence:

>1488_bases
GTGAGGGGCATGAGAATTGATGCTTATATCGCTGAGCACGCCAGAAAAAATCCTGATCAGCTAGCTCTTGAAGACGGTAA
AAAGTCCATTTCTTACAGTTGCCTTGATGAGGACATAAATAAGGTTGCCTCATCTCTGGATGGCTTTGAGCACTGCAAAT
TTGCAATACTGGCAGAGTCTGGAATACAGTATGTCAAAATACTTATGGCAGTATACAGGTCAGGCAATATTGCAATACCT
CTGCCAATCGAAATGCAGGGGAGAAGCCTTGAAAGAATTCTTGATACTGCATGCATTAACAACATAATTGCAACGAAAAA
ACAGTATTCAAGGTTTGGAGATGGATTTTTTGAGCGTTTTGGAACCGTAATCGTCGTTTCGGATGATACTTCAGTAGAGA
CGCTCAGCAGAAGAATGATCCTCGAAAGAAATGATCCCAGGCTTCGGCTGGTGCTTTACACCTCGGGAACAACAGGGACT
CCCAAGGGAGTTATGCTGAGCGATAAAAACCTGGTCTCAAATGCCAGGTCAATAACCGGGATTCTCAGCATAGGCTCAAA
AGATAAAGCAGCACTGGTAATCTCTCCCCATCACGCCTTCGGGAATTCGATAATTAACTCCCATCTGATGGCTGGAAGCT
CAATTCAAATCGGGACAATGAATTTCATAGACCCCGTTTTCAGTCTCATCGAATCCGGAGTATCCATATTCTATGGAGTG
CCCAGCACCTATCGCATTCTTCTCAAATATCCGGAGAGGTTCCGAAAAGCCTTTCAAAATGTCAGGACTGCCGCCTCCGC
TGGCGGAGCCATGGACAGAACCGTTGTAAGGAGTATCAGGGAATTAAGTCCGGATACGCAGATCCTGCCAATGTACGGCC
AGACCGAAGCTACGGCAAGGCTTTCCTACCTGCCTGCAGAAGACGTGGACGAGTTTATCGACACGATAGGAAAAGCAATT
CCCGGCGTCACACTGGAAGTCTTTGATACTGAAAACAGGCCGGTAGAGCCCGGCGTCACAGGTGAGCTTGTTGCCAGAGG
GGATAATATTCTGCTTGGCTATCTGAATGACGAGGCTGCAACGGAAAAAAAGATCATTGACGGGTGGCTGCATACAGGGG
ATCTGGCACAGAAACTTCGCTGCGGGTATATAAAGCTGCTTGGGCGCAAGGACGATCTCATCAAAATAGGGGATCACCGG
GTTAACCCGAGAGAGATTGAAAAAGAAATAGAGATGAATAATGAGGTTTCCAGTGTTTTTGTTGTGCCCGTATCCCATGA
GCTAATGGGAAATGCAATCAGCCTCATGGTCATACCAAATGAGAGAACCGAGATCGATACAATTTTTTCTTTTTGCAGGA
AGAACCTGCCGGGTTATCTCTATCCGAGGGAGATCCTCTTCATTGACCATCTCCCACTGAGCGAGAACGGGAAAATATCC
AACAGGTCTATTATAGAGGAGTACCAGCATGTCAAAGCTAGTATGTAA

Upstream 100 bases:

>100_bases
TCTGTGAAACATATTCCATCGAAATTCCTGAAGATATGTTGACTCCGGAGAATTTCGATTCTTTACAGGGGATTACCAAC
CTGATAACCAGACTGGCGAA

Downstream 100 bases:

>100_bases
GCGGTGTGTTCTGGATTCTGATATTCCAGGCATAGAAATCAATAAAGAAAGCGGTCTCTGCCATTTTTGCGAGACATATG
TCCCTCTTTCTTTACAGGAA

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRGMRIDAYIAEHARKNPDQLALEDGKKSISYSCLDEDINKVASSLDGFEHCKFAILAESGIQYVKILMAVYRSGNIAIP
LPIEMQGRSLERILDTACINNIIATKKQYSRFGDGFFERFGTVIVVSDDTSVETLSRRMILERNDPRLRLVLYTSGTTGT
PKGVMLSDKNLVSNARSITGILSIGSKDKAALVISPHHAFGNSIINSHLMAGSSIQIGTMNFIDPVFSLIESGVSIFYGV
PSTYRILLKYPERFRKAFQNVRTAASAGGAMDRTVVRSIRELSPDTQILPMYGQTEATARLSYLPAEDVDEFIDTIGKAI
PGVTLEVFDTENRPVEPGVTGELVARGDNILLGYLNDEAATEKKIIDGWLHTGDLAQKLRCGYIKLLGRKDDLIKIGDHR
VNPREIEKEIEMNNEVSSVFVVPVSHELMGNAISLMVIPNERTEIDTIFSFCRKNLPGYLYPREILFIDHLPLSENGKIS
NRSIIEEYQHVKASM

Sequences:

>Translated_495_residues
MRGMRIDAYIAEHARKNPDQLALEDGKKSISYSCLDEDINKVASSLDGFEHCKFAILAESGIQYVKILMAVYRSGNIAIP
LPIEMQGRSLERILDTACINNIIATKKQYSRFGDGFFERFGTVIVVSDDTSVETLSRRMILERNDPRLRLVLYTSGTTGT
PKGVMLSDKNLVSNARSITGILSIGSKDKAALVISPHHAFGNSIINSHLMAGSSIQIGTMNFIDPVFSLIESGVSIFYGV
PSTYRILLKYPERFRKAFQNVRTAASAGGAMDRTVVRSIRELSPDTQILPMYGQTEATARLSYLPAEDVDEFIDTIGKAI
PGVTLEVFDTENRPVEPGVTGELVARGDNILLGYLNDEAATEKKIIDGWLHTGDLAQKLRCGYIKLLGRKDDLIKIGDHR
VNPREIEKEIEMNNEVSSVFVVPVSHELMGNAISLMVIPNERTEIDTIFSFCRKNLPGYLYPREILFIDHLPLSENGKIS
NRSIIEEYQHVKASM
>Mature_495_residues
MRGMRIDAYIAEHARKNPDQLALEDGKKSISYSCLDEDINKVASSLDGFEHCKFAILAESGIQYVKILMAVYRSGNIAIP
LPIEMQGRSLERILDTACINNIIATKKQYSRFGDGFFERFGTVIVVSDDTSVETLSRRMILERNDPRLRLVLYTSGTTGT
PKGVMLSDKNLVSNARSITGILSIGSKDKAALVISPHHAFGNSIINSHLMAGSSIQIGTMNFIDPVFSLIESGVSIFYGV
PSTYRILLKYPERFRKAFQNVRTAASAGGAMDRTVVRSIRELSPDTQILPMYGQTEATARLSYLPAEDVDEFIDTIGKAI
PGVTLEVFDTENRPVEPGVTGELVARGDNILLGYLNDEAATEKKIIDGWLHTGDLAQKLRCGYIKLLGRKDDLIKIGDHR
VNPREIEKEIEMNNEVSSVFVVPVSHELMGNAISLMVIPNERTEIDTIFSFCRKNLPGYLYPREILFIDHLPLSENGKIS
NRSIIEEYQHVKASM

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=540, Percent_Identity=23.5185185185185, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI187761345, Length=511, Percent_Identity=23.091976516634, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI187761343, Length=511, Percent_Identity=23.091976516634, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI45580730, Length=364, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI57165410, Length=446, Percent_Identity=23.7668161434978, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI57165412, Length=450, Percent_Identity=23.7777777777778, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI28416953, Length=359, Percent_Identity=24.2339832869081, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI38505220, Length=456, Percent_Identity=22.1491228070175, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI40807491, Length=356, Percent_Identity=24.438202247191, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI42544132, Length=354, Percent_Identity=22.316384180791, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI157311624, Length=495, Percent_Identity=23.030303030303, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI157311622, Length=495, Percent_Identity=23.030303030303, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI115511026, Length=464, Percent_Identity=23.4913793103448, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI58082049, Length=459, Percent_Identity=22.4400871459695, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788107, Length=528, Percent_Identity=26.5151515151515, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=25.3846153846154, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786801, Length=517, Percent_Identity=25.1450676982592, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789201, Length=369, Percent_Identity=24.390243902439, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI221142682, Length=361, Percent_Identity=24.0997229916898, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786810, Length=491, Percent_Identity=21.5885947046843, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=355, Percent_Identity=29.2957746478873, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=355, Percent_Identity=29.2957746478873, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=355, Percent_Identity=29.2957746478873, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=396, Percent_Identity=27.020202020202, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=359, Percent_Identity=26.7409470752089, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=360, Percent_Identity=25, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=379, Percent_Identity=27.7044854881267, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=357, Percent_Identity=26.0504201680672, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=378, Percent_Identity=26.4550264550265, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=287, Percent_Identity=27.1777003484321, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=392, Percent_Identity=22.7040816326531, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=350, Percent_Identity=22.8571428571429, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556356, Length=348, Percent_Identity=26.1494252873563, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560140, Length=353, Percent_Identity=22.0963172804533, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=24.7126436781609, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=374, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=354, Percent_Identity=25.9887005649718, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18859661, Length=347, Percent_Identity=25.0720461095101, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355181, Length=361, Percent_Identity=26.8698060941828, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648676, Length=508, Percent_Identity=24.4094488188976, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648260, Length=342, Percent_Identity=24.8538011695906, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653035, Length=463, Percent_Identity=22.2462203023758, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922652, Length=357, Percent_Identity=26.0504201680672, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130357, Length=353, Percent_Identity=25.4957507082153, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161076582, Length=482, Percent_Identity=21.9917012448133, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356947, Length=363, Percent_Identity=24.5179063360882, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24656500, Length=352, Percent_Identity=26.1363636363636, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648257, Length=354, Percent_Identity=22.8813559322034, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17933690, Length=351, Percent_Identity=24.5014245014245, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648253, Length=370, Percent_Identity=23.2432432432432, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648255, Length=370, Percent_Identity=23.2432432432432, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365686, Length=437, Percent_Identity=23.5697940503432, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358303, Length=374, Percent_Identity=25.1336898395722, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356441, Length=319, Percent_Identity=23.8244514106583, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55042; Mature: 55042

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGMRIDAYIAEHARKNPDQLALEDGKKSISYSCLDEDINKVASSLDGFEHCKFAILAES
CCCCEEHHHHHHHHCCCCCCEEHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCEEEEEECC
GIQYVKILMAVYRSGNIAIPLPIEMQGRSLERILDTACINNIIATKKQYSRFGDGFFERF
CHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GTVIVVSDDTSVETLSRRMILERNDPRLRLVLYTSGTTGTPKGVMLSDKNLVSNARSITG
CEEEEEECCCCHHHHHHHHHEECCCCEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHEE
ILSIGSKDKAALVISPHHAFGNSIINSHLMAGSSIQIGTMNFIDPVFSLIESGVSIFYGV
EEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCEEEECC
PSTYRILLKYPERFRKAFQNVRTAASAGGAMDRTVVRSIRELSPDTQILPMYGQTEATAR
CHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHE
LSYLPAEDVDEFIDTIGKAIPGVTLEVFDTENRPVEPGVTGELVARGDNILLGYLNDEAA
EECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEEEECCCHH
TEKKIIDGWLHTGDLAQKLRCGYIKLLGRKDDLIKIGDHRVNPREIEKEIEMNNEVSSVF
HHHHHHHHHCCCHHHHHHHHHCHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEE
VVPVSHELMGNAISLMVIPNERTEIDTIFSFCRKNLPGYLYPREILFIDHLPLSENGKIS
EEECCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
NRSIIEEYQHVKASM
CHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGMRIDAYIAEHARKNPDQLALEDGKKSISYSCLDEDINKVASSLDGFEHCKFAILAES
CCCCEEHHHHHHHHCCCCCCEEHHCCCCCCCHHHHHHHHHHHHHHCCCHHHCEEEEEECC
GIQYVKILMAVYRSGNIAIPLPIEMQGRSLERILDTACINNIIATKKQYSRFGDGFFERF
CHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GTVIVVSDDTSVETLSRRMILERNDPRLRLVLYTSGTTGTPKGVMLSDKNLVSNARSITG
CEEEEEECCCCHHHHHHHHHEECCCCEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHEE
ILSIGSKDKAALVISPHHAFGNSIINSHLMAGSSIQIGTMNFIDPVFSLIESGVSIFYGV
EEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCEEEECC
PSTYRILLKYPERFRKAFQNVRTAASAGGAMDRTVVRSIRELSPDTQILPMYGQTEATAR
CHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHE
LSYLPAEDVDEFIDTIGKAIPGVTLEVFDTENRPVEPGVTGELVARGDNILLGYLNDEAA
EECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCEEEEEECCCHH
TEKKIIDGWLHTGDLAQKLRCGYIKLLGRKDDLIKIGDHRVNPREIEKEIEMNNEVSSVF
HHHHHHHHHCCCHHHHHHHHHCHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEE
VVPVSHELMGNAISLMVIPNERTEIDTIFSFCRKNLPGYLYPREILFIDHLPLSENGKIS
EEECCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
NRSIIEEYQHVKASM
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]