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The map label for this gene is mhpC [H]

Identifier: 21226631

GI number: 21226631

Start: 656505

End: 657317

Strand: Reverse

Name: mhpC [H]

Synonym: MM_0529

Alternate gene names: 21226631

Gene position: 657317-656505 (Counterclockwise)

Preceding gene: 21226632

Following gene: 21226630

Centisome position: 16.05

GC content: 48.09

Gene sequence:

>813_bases
ATGACCACCGGGTACGCAGACTCTGGAGACGGAAAGCTTTACTATGAAATCGAGGGAAAGGGCGAGGTTCTTGTGCTCAT
CCATGCCGGGTTTGTTGACAGTGGCATGTGGGAAGAGCAGTGGGAGTCTTTTACCCGAAATTACAGGGTTCTTCGCTTTG
ATATGCGCGGGTTTGGAAAATCCGATCCTGTAACAGGACCCGTTTCCCACCGACATGACCTGTACCACCTGCTTCAGGGC
CTGGGAATTAAACATGCACATTTACTGGGCTGTTCCATGGGCGGAGAAACAGTTATTGATTTTACGCTCGAACACCCTGA
AATGGTTCTGTCTCTAACCGTTGTAAGCGGGATTCCGGGAAGTTTTGAAATGACAGGGGAGCCCCCATCTGAGATTCTGG
AGATGCTTCAAGCTCTCGAAAAGGGGGATCTGGACCTTGTGAGTGACCTGCAAATCCGCCTCTGGGTTGATGGCACTTTC
CGGCAGCCTGAACAGGTCAGCCCTTCAGTTCGTAAGCATGCAGCTGAAATGAACAGGGTTGCGGTTAAAAACGGCACATG
GGCAATTGCCGATGCCAGCCCTCTTAACCCGCTCAGTCCACCGGCTGCCGGGAGGCTGGATGAGATAAAAGTTCCTGTGC
TTATTATTGTCGGGTCTCTTGATAATCCTGAAATCCTCAGGGCTGCTGATCTGCTTGAAAATAAAATCGAAAATGCAAAA
AAAGTTATAATGCCAGATTGTGCTCATGTGCCAAATATGGAAAAACCTGAAGAATTCAACCGGAATGTACTTAATTTTCT
TAGAAATTTATAA

Upstream 100 bases:

>100_bases
CCGGAAAGCATATGCAAAGTTCAGGAAGGAAAAAGGCAGGGACTGAACAGGCACTAATCAGGAAGATGATCGGGCTTAAT
TACACAGGAGACAACAGGAA

Downstream 100 bases:

>100_bases
GGTCATAAAACACAGAGATTTATAATAGAAAGCGGTGGATTTCTCCAGATCAACATTTTTTTAATACTTTTACAATACTT
CTATATTATTTTTATAATTT

Product: hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTTGYADSGDGKLYYEIEGKGEVLVLIHAGFVDSGMWEEQWESFTRNYRVLRFDMRGFGKSDPVTGPVSHRHDLYHLLQG
LGIKHAHLLGCSMGGETVIDFTLEHPEMVLSLTVVSGIPGSFEMTGEPPSEILEMLQALEKGDLDLVSDLQIRLWVDGTF
RQPEQVSPSVRKHAAEMNRVAVKNGTWAIADASPLNPLSPPAAGRLDEIKVPVLIIVGSLDNPEILRAADLLENKIENAK
KVIMPDCAHVPNMEKPEEFNRNVLNFLRNL

Sequences:

>Translated_270_residues
MTTGYADSGDGKLYYEIEGKGEVLVLIHAGFVDSGMWEEQWESFTRNYRVLRFDMRGFGKSDPVTGPVSHRHDLYHLLQG
LGIKHAHLLGCSMGGETVIDFTLEHPEMVLSLTVVSGIPGSFEMTGEPPSEILEMLQALEKGDLDLVSDLQIRLWVDGTF
RQPEQVSPSVRKHAAEMNRVAVKNGTWAIADASPLNPLSPPAAGRLDEIKVPVLIIVGSLDNPEILRAADLLENKIENAK
KVIMPDCAHVPNMEKPEEFNRNVLNFLRNL
>Mature_269_residues
TTGYADSGDGKLYYEIEGKGEVLVLIHAGFVDSGMWEEQWESFTRNYRVLRFDMRGFGKSDPVTGPVSHRHDLYHLLQGL
GIKHAHLLGCSMGGETVIDFTLEHPEMVLSLTVVSGIPGSFEMTGEPPSEILEMLQALEKGDLDLVSDLQIRLWVDGTFR
QPEQVSPSVRKHAAEMNRVAVKNGTWAIADASPLNPLSPPAAGRLDEIKVPVLIIVGSLDNPEILRAADLLENKIENAKK
VIMPDCAHVPNMEKPEEFNRNVLNFLRNL

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=275, Percent_Identity=30.5454545454545, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29952; Mature: 29820

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGYADSGDGKLYYEIEGKGEVLVLIHAGFVDSGMWEEQWESFTRNYRVLRFDMRGFGK
CCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCC
SDPVTGPVSHRHDLYHLLQGLGIKHAHLLGCSMGGETVIDFTLEHPEMVLSLTVVSGIPG
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCEEEEEEEECCCCC
SFEMTGEPPSEILEMLQALEKGDLDLVSDLQIRLWVDGTFRQPEQVSPSVRKHAAEMNRV
CEEECCCCHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCHHCCHHHHHHHHHHHHE
AVKNGTWAIADASPLNPLSPPAAGRLDEIKVPVLIIVGSLDNPEILRAADLLENKIENAK
EEECCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KVIMPDCAHVPNMEKPEEFNRNVLNFLRNL
EEECCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTGYADSGDGKLYYEIEGKGEVLVLIHAGFVDSGMWEEQWESFTRNYRVLRFDMRGFGK
CCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCC
SDPVTGPVSHRHDLYHLLQGLGIKHAHLLGCSMGGETVIDFTLEHPEMVLSLTVVSGIPG
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCEEEEEEEECCCCC
SFEMTGEPPSEILEMLQALEKGDLDLVSDLQIRLWVDGTFRQPEQVSPSVRKHAAEMNRV
CEEECCCCHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCHHCCHHHHHHHHHHHHE
AVKNGTWAIADASPLNPLSPPAAGRLDEIKVPVLIIVGSLDNPEILRAADLLENKIENAK
EEECCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KVIMPDCAHVPNMEKPEEFNRNVLNFLRNL
EEECCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA