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The map label for this gene is endA

Identifier: 21226614

GI number: 21226614

Start: 636450

End: 637511

Strand: Reverse

Name: endA

Synonym: MM_0512

Alternate gene names: 21226614

Gene position: 637511-636450 (Counterclockwise)

Preceding gene: 21226615

Following gene: 21226613

Centisome position: 15.56

GC content: 47.55

Gene sequence:

>1062_bases
GTGAATCTATTGAAAACACAACTTAAAGGGGACAGGGTCCTCGCAGGAAAGGAAGCTGTAGCCGAACTTTACAAAACAGG
TTATTTCGGGCGTCCAAAAGACGACGGGCTTGAACTTTCACTTGTGGAGGCTGCGTACCTTCAGTCCCGAGGAAAACTCG
ACATCGAGCTTGAAGGAAAACTGCTAGGTTTCAGGGCATTTTTTGAACAGGCTTCTCTGAGGCAGCAGAATTTCGAGCTT
AAGTACATCGTTTACAAGGACCTCAAAGAAAGAGGATATTATGTCCAGCCCTCAGCCGCGGATTTCAGGGTGTACCCTCG
AGGCAGCCACCCGGGAAAAAGCGCAGCAAAAATTTTTGTTCACGTCCTTTCCGAAAGGCAGCCACTTTCTGTAAAACTTT
TGCAGGAATCCGTCGCTTCTGCAGAAAATGTCCACAAGCAGTTCATCCTGGCTGTTGTGGACGAAGAAAGTGACCTCACC
TTTTACGAAATCAAATCCGCTTCTCCCAAAGGGGAAATGCCTGAGCCCTTCCCTGCAGTCAAAGCCGATGCAACTTTCCT
TGAAGACAGGGTAATAGCCTGGGATGCAGAAGCCTCCGGGGCACTTTATTCCAGGGGCTTTTACGGGAAAATGCTTGATC
CTGAAAGGCTGCAGCTTTCGCTGGTTGAGTCCCTCTACCTTTTTTCGAGAGGGGTTATAGTCGTCAGGGACAGGAAAGGA
AAAATCTTCTCATTCGATGAGTTTGTTGAAAAAGCTTCGGAAATCGAAGGCTCTTTCCTGCGCAAATACAGCGCATATAA
AGCCCTCAGGGACTCAGGGCATGTGGTCAAGACAGGATTTAAGTTCGGGACACATTTCAGAGTCTACAGGAAAGTCGAAT
CAATAGAAAAAATTCCCCACTCAGAATACCTTGTGAACGTTATCCCTGAAGATTACGAGTTCAGGCTTCCGGTCATGTCC
GGGGCTGTAAGGCTCGCAAACAGCGTAAGAAAAAGAATGCTCTTTGCGGTCGAGAAAGGGGAAGAAGTCGAGTACCTTGA
TATAGGCAGGGTCAAAATGTAA

Upstream 100 bases:

>100_bases
CAGGATAAAAATCCGGTCCGGTGCAAATCAGACTGTTAAAAACCGGGATCTTACGAAATAAATTTTTATGTAACTTATTT
TCATCCCATTTTCTTTTCAG

Downstream 100 bases:

>100_bases
TTCCGTTTGATTCTATTGATCTCATATTGCTTCTGTATTATTTCTTAAAGAACGAATGTGTTTTATTGACTTTACTTCTG
GCTTGTTACGTTTTCATCCC

Product: tRNA splicing endonuclease

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNLLKTQLKGDRVLAGKEAVAELYKTGYFGRPKDDGLELSLVEAAYLQSRGKLDIELEGKLLGFRAFFEQASLRQQNFEL
KYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFVHVLSERQPLSVKLLQESVASAENVHKQFILAVVDEESDLT
FYEIKSASPKGEMPEPFPAVKADATFLEDRVIAWDAEASGALYSRGFYGKMLDPERLQLSLVESLYLFSRGVIVVRDRKG
KIFSFDEFVEKASEIEGSFLRKYSAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPHSEYLVNVIPEDYEFRLPVMS
GAVRLANSVRKRMLFAVEKGEEVEYLDIGRVKM

Sequences:

>Translated_353_residues
MNLLKTQLKGDRVLAGKEAVAELYKTGYFGRPKDDGLELSLVEAAYLQSRGKLDIELEGKLLGFRAFFEQASLRQQNFEL
KYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFVHVLSERQPLSVKLLQESVASAENVHKQFILAVVDEESDLT
FYEIKSASPKGEMPEPFPAVKADATFLEDRVIAWDAEASGALYSRGFYGKMLDPERLQLSLVESLYLFSRGVIVVRDRKG
KIFSFDEFVEKASEIEGSFLRKYSAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPHSEYLVNVIPEDYEFRLPVMS
GAVRLANSVRKRMLFAVEKGEEVEYLDIGRVKM
>Mature_353_residues
MNLLKTQLKGDRVLAGKEAVAELYKTGYFGRPKDDGLELSLVEAAYLQSRGKLDIELEGKLLGFRAFFEQASLRQQNFEL
KYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFVHVLSERQPLSVKLLQESVASAENVHKQFILAVVDEESDLT
FYEIKSASPKGEMPEPFPAVKADATFLEDRVIAWDAEASGALYSRGFYGKMLDPERLQLSLVESLYLFSRGVIVVRDRKG
KIFSFDEFVEKASEIEGSFLRKYSAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPHSEYLVNVIPEDYEFRLPVMS
GAVRLANSVRKRMLFAVEKGEEVEYLDIGRVKM

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_METMA (Q8PZI1)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632536.1
- ProteinModelPortal:   Q8PZI1
- SMR:   Q8PZI1
- GeneID:   1478854
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0512
- NMPDR:   fig|192952.1.peg.512
- HOGENOM:   HBG538413
- OMA:   GADFRTY
- ProtClustDB:   PRK09300
- BioCyc:   MMAZ192952:MM0512-MONOMER
- BRENDA:   3.1.27.9
- HAMAP:   MF_01834
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- Gene3D:   G3DSA:3.40.1350.10
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 40192; Mature: 40192

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: ACT_SITE 289-289 ACT_SITE 300-300 ACT_SITE 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLKTQLKGDRVLAGKEAVAELYKTGYFGRPKDDGLELSLVEAAYLQSRGKLDIELEGK
CCCCHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCE
LLGFRAFFEQASLRQQNFELKYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFV
EEHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHH
HVLSERQPLSVKLLQESVASAENVHKQFILAVVDEESDLTFYEIKSASPKGEMPEPFPAV
HHHCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCC
KADATFLEDRVIAWDAEASGALYSRGFYGKMLDPERLQLSLVESLYLFSRGVIVVRDRKG
CCCHHHHHCEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCC
KIFSFDEFVEKASEIEGSFLRKYSAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPH
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
SEYLVNVIPEDYEFRLPVMSGAVRLANSVRKRMLFAVEKGEEVEYLDIGRVKM
CCEEEEECCCCCEEECHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure
MNLLKTQLKGDRVLAGKEAVAELYKTGYFGRPKDDGLELSLVEAAYLQSRGKLDIELEGK
CCCCHHHCCCCEEEECHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCE
LLGFRAFFEQASLRQQNFELKYIVYKDLKERGYYVQPSAADFRVYPRGSHPGKSAAKIFV
EEHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHH
HVLSERQPLSVKLLQESVASAENVHKQFILAVVDEESDLTFYEIKSASPKGEMPEPFPAV
HHHCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCC
KADATFLEDRVIAWDAEASGALYSRGFYGKMLDPERLQLSLVESLYLFSRGVIVVRDRKG
CCCHHHHHCEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCC
KIFSFDEFVEKASEIEGSFLRKYSAYKALRDSGHVVKTGFKFGTHFRVYRKVESIEKIPH
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
SEYLVNVIPEDYEFRLPVMSGAVRLANSVRKRMLFAVEKGEEVEYLDIGRVKM
CCEEEEECCCCCEEECHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824