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The map label for this gene is otaB [H]

Identifier: 21226143

GI number: 21226143

Start: 52774

End: 53604

Strand: Direct

Name: otaB [H]

Synonym: MM_0041

Alternate gene names: 21226143

Gene position: 52774-53604 (Clockwise)

Preceding gene: 21226142

Following gene: 21226144

Centisome position: 1.29

GC content: 47.17

Gene sequence:

>831_bases
GTGATTTTCTCCAAAATTCCTATAGGAGATTACGTTGAGATTGCAGTCTACTGGATCGAGCTCAATCTTGGCTGGCTCCT
GGACATTATAAGCGATTTCCTTGGTTTTATGATTACTGGATTCAGGGACTTTCTGATGCTTTTCCCACCTCTCATTTTCG
CGCTGCTTATTGCTGCCCTGGCTTATTTCACAGGCAAGAAAAATCTGAAACTTGCGGTGGGAACTTTTGTCGGTCTCCTG
TTGATTGAGAATTTGCGACTATGGACACTTTCAATGGAAACACTTTCCCTTGTGATCTCCTCGGCATTTCTGGCACTGGT
TATAGGAATTCCCCTGGGAATTCTCGCTGCTAAAAGTGATTTCTTCTTTAACGTCCTGAAGCCGATCCTGGACCTTATGC
AGACCATGCCTTCTTTTGTTTACCTCATTCCCGCCCTGATCTTCTTCGGGCTTGGAAACGTCCCGGGCATGATTGCAACC
CTTGTGTTTGCAATGCCTCCTACCATTCGTTTGACCAATCTTGGAATCCGCCAGGTTCCTCGGGAGCTTAAGGAGGTTGC
GGATGCCTTTGGGTCAACACCCTGGCAAAAGCTTGTTAAAGTTGAGCTGCCTGCGGCCCTGCCGACAATTATGGCAGGAG
TTAACCAGTGTATTATGCTTTCCCTTTCAATGGTAGTTATAGCCGCCATGATAGGAGCAAGAGGTCTTGGTTACCAGGTG
CTCTTCGGGATACAGAGGGTTGACATAGGCCTTGGCTTCGAAGCAGGGCTCGGAATTGTTATTCTTGCTGTGATCCTTGA
CAGAATCACTCAAAACCTCAGTCCGAAGTAA

Upstream 100 bases:

>100_bases
TAGACAACAAAGGACAGCAGGAAAGCCCCGCTTCAGAGAAGGGACTTTCTTCCGAAGTGGAAGATATTAGTCAAAAATCT
GAAAATGTGGAGGTTAAAAT

Downstream 100 bases:

>100_bases
AGATTGGGTATCTTTTCCATTTATCGATTCACAATGTGTTTATATCGATATACTCTTTCCCAGGCCGTTTGGGAATTTTA
TATTAGACACAACAACGGCC

Product: glycine betaine transporter, permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIFSKIPIGDYVEIAVYWIELNLGWLLDIISDFLGFMITGFRDFLMLFPPLIFALLIAALAYFTGKKNLKLAVGTFVGLL
LIENLRLWTLSMETLSLVISSAFLALVIGIPLGILAAKSDFFFNVLKPILDLMQTMPSFVYLIPALIFFGLGNVPGMIAT
LVFAMPPTIRLTNLGIRQVPRELKEVADAFGSTPWQKLVKVELPAALPTIMAGVNQCIMLSLSMVVIAAMIGARGLGYQV
LFGIQRVDIGLGFEAGLGIVILAVILDRITQNLSPK

Sequences:

>Translated_276_residues
MIFSKIPIGDYVEIAVYWIELNLGWLLDIISDFLGFMITGFRDFLMLFPPLIFALLIAALAYFTGKKNLKLAVGTFVGLL
LIENLRLWTLSMETLSLVISSAFLALVIGIPLGILAAKSDFFFNVLKPILDLMQTMPSFVYLIPALIFFGLGNVPGMIAT
LVFAMPPTIRLTNLGIRQVPRELKEVADAFGSTPWQKLVKVELPAALPTIMAGVNQCIMLSLSMVVIAAMIGARGLGYQV
LFGIQRVDIGLGFEAGLGIVILAVILDRITQNLSPK
>Mature_276_residues
MIFSKIPIGDYVEIAVYWIELNLGWLLDIISDFLGFMITGFRDFLMLFPPLIFALLIAALAYFTGKKNLKLAVGTFVGLL
LIENLRLWTLSMETLSLVISSAFLALVIGIPLGILAAKSDFFFNVLKPILDLMQTMPSFVYLIPALIFFGLGNVPGMIAT
LVFAMPPTIRLTNLGIRQVPRELKEVADAFGSTPWQKLVKVELPAALPTIMAGVNQCIMLSLSMVVIAAMIGARGLGYQV
LFGIQRVDIGLGFEAGLGIVILAVILDRITQNLSPK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=266, Percent_Identity=45.1127819548872, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788449, Length=187, Percent_Identity=28.3422459893048, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=30.3225806451613, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30180; Mature: 30180

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSKIPIGDYVEIAVYWIELNLGWLLDIISDFLGFMITGFRDFLMLFPPLIFALLIAAL
CCCCCCCCCCHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYFTGKKNLKLAVGTFVGLLLIENLRLWTLSMETLSLVISSAFLALVIGIPLGILAAKSD
HHHCCCCCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFFNVLKPILDLMQTMPSFVYLIPALIFFGLGNVPGMIATLVFAMPPTIRLTNLGIRQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCHHHHH
RELKEVADAFGSTPWQKLVKVELPAALPTIMAGVNQCIMLSLSMVVIAAMIGARGLGYQV
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LFGIQRVDIGLGFEAGLGIVILAVILDRITQNLSPK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIFSKIPIGDYVEIAVYWIELNLGWLLDIISDFLGFMITGFRDFLMLFPPLIFALLIAAL
CCCCCCCCCCHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYFTGKKNLKLAVGTFVGLLLIENLRLWTLSMETLSLVISSAFLALVIGIPLGILAAKSD
HHHCCCCCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFFNVLKPILDLMQTMPSFVYLIPALIFFGLGNVPGMIATLVFAMPPTIRLTNLGIRQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCHHHHH
RELKEVADAFGSTPWQKLVKVELPAALPTIMAGVNQCIMLSLSMVVIAAMIGARGLGYQV
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LFGIQRVDIGLGFEAGLGIVILAVILDRITQNLSPK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]