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The map label for this gene is phoB [H]

Identifier: 209695880

GI number: 209695880

Start: 2629731

End: 2630423

Strand: Reverse

Name: phoB [H]

Synonym: VSAL_I2457

Alternate gene names: 209695880

Gene position: 2630423-2629731 (Counterclockwise)

Preceding gene: 209695890

Following gene: 209695879

Centisome position: 79.11

GC content: 41.85

Gene sequence:

>693_bases
ATGTCTAGAAGGATACTTGTCGTAGAAGATGAAGCGCCAATTCGTGAGATGCTGTGCTTTGTATTAGAGCAGAAAGGATA
CGAACCAATTGAAGCGGAAGATTATGATACTGCGCTTGCTTTACTCTGCGAACCCTACCCAGATTTAATTTTGTTGGATT
GGATGTTACCTGGTGGCTCTGGCATTAATTTAATCAAACATCTTAAGCGTGATGAATTTACGAAAAAGATCCCTGTTGTG
ATGTTAACCGCTCGGGGTGAAGAAGAAGATAAAGTGCGTGGCCTTGAAGTGGGGGCGGATGACTACATAACAAAACCATT
CTCACCAAAAGAATTAATGGCGCGTTTAAAAGCGGTAATTCGTCGTGTTTCACCGACTTCTGTTGAAGAATTAATTGATG
TTCAAGGATTAAAACTTGATCCGGTTTCTCATCGAGTGACGGCAAATGATAAGCCGTTAGACATGGGACCAACAGAATTT
AAAATGCTGCATTTCTTTATGACACATCAAGAACGTGTATATAGTCGTGAACAGTTATTGAATAACGTATGGGGCACTAA
CGTCTACGTTGAAGATCGCACTGTTGATGTTCATATTCGTCGTCTTCGTAAAGCGCTAGAAGCAGAAGGGCATGATCGTT
TGGTTCAAACGGTTCGTGGTGCTGGCTACCGTTTTTCTGTGCAATGCGCATAA

Upstream 100 bases:

>100_bases
TGATGAAATTATGAACTTTTCTTTTCAATAGTCACAAAAGTGTCATAATTACCTCTAATAATAGCGTTATAAATTTTGTT
GGTTAAATTAGGTAAAGATA

Downstream 100 bases:

>100_bases
CCGTCAATTATTAACGTAAAGGTAGGCTAAATGGTTGAGCGTCTGTCATGGAAAAAGCTGGTTGGAGAATTGGCTTTTTT
CTATGTTCCTTGGATTATTG

Product: response regulator homolog PhoB (phosphate regulon transcriptional regulatory protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSRRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDTALALLCEPYPDLILLDWMLPGGSGINLIKHLKRDEFTKKIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVIRRVSPTSVEELIDVQGLKLDPVSHRVTANDKPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEAEGHDRLVQTVRGAGYRFSVQCA

Sequences:

>Translated_230_residues
MSRRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDTALALLCEPYPDLILLDWMLPGGSGINLIKHLKRDEFTKKIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVIRRVSPTSVEELIDVQGLKLDPVSHRVTANDKPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEAEGHDRLVQTVRGAGYRFSVQCA
>Mature_229_residues
SRRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDTALALLCEPYPDLILLDWMLPGGSGINLIKHLKRDEFTKKIPVVM
LTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVIRRVSPTSVEELIDVQGLKLDPVSHRVTANDKPLDMGPTEFK
MLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEAEGHDRLVQTVRGAGYRFSVQCA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=79.8245614035088, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=40.1785714285714, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=35.8078602620087, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=35.7142857142857, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=36.322869955157, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=34.6846846846847, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=37.5, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.117903930131, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=35.6223175965665, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=33.0316742081448, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=32.1739130434783, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=29.7872340425532, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=38.5321100917431, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=28.6885245901639, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26408; Mature: 26277

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDTALALLCEPYPDLILLDWMLPGGS
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCC
GINLIKHLKRDEFTKKIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVI
CHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVSPTSVEELIDVQGLKLDPVSHRVTANDKPLDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCCCCHHHHHHHCCCEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEAEGHDRLVQTVRGAGYRFSVQCA
HHHCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEC
>Mature Secondary Structure 
SRRILVVEDEAPIREMLCFVLEQKGYEPIEAEDYDTALALLCEPYPDLILLDWMLPGGS
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCC
GINLIKHLKRDEFTKKIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELMARLKAVI
CHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVSPTSVEELIDVQGLKLDPVSHRVTANDKPLDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCCCCHHHHHHHCCCEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEAEGHDRLVQTVRGAGYRFSVQCA
HHHCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]