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The map label for this gene is rnhB

Identifier: 209695837

GI number: 209695837

Start: 2583148

End: 2583771

Strand: Reverse

Name: rnhB

Synonym: VSAL_I2413

Alternate gene names: 209695837

Gene position: 2583771-2583148 (Counterclockwise)

Preceding gene: 209695838

Following gene: 209695836

Centisome position: 77.7

GC content: 40.87

Gene sequence:

>624_bases
ATGACAAAGAAAATAGATTCTAAAGAATTACCTCCATTTGAGTACCCACAAGGCTATCAGTTATTTGCGGGCGTTGATGA
AGTTGGACGCGGCCCGTTAGTGGGTGCGGTTGTGACTGCTGCTGTTATTTTAGATCCTAATAATCCAATTGAAGGATTAA
CGGATTCAAAAAAATTGACAGAAAAAAAACGTGATTTGTTATTTCCTGAAATTCAAGAAAAAGCGTTAGCTTGGTCTTTA
GGGCGATGTGAAGCACATGAGATCGATGAGTTGAATATTTTACAAGCCACAATGGTTGCGATGCAAAGAGCTATTGCTGG
GTTGAAGGTTACTCCTGATTTCGCTCTAATTGATGGGAATAAGGTGCCTGAATTGCCAATGGCTGGTTTAGCGGTTGTAA
AAGGTGATTTACGCGTTCAAGAGATCAGCGCGGCTTCAATTCTTGCTAAGGTTACTCGTGACAGAGAAATGGAAGTATTG
GATAAAGAGTTCCCTCAATATGGCTTTGCTAAACACAAAGGTTATCCAACCAAGGCGCATTTTGCTGCGATTGAAGAGCA
CGGTGTTATTTCAGAGCATCGTCGTAGTTTTAAACCTGTAAAACGTGTTTTAGGGATCGAGTAA

Upstream 100 bases:

>100_bases
AGATTTACAGAGATGCACCAGTGGATTAAAAAAGATGCAGATAAACAAGCTGCTAATGCCGTCTTACGTCTAATTAATAA
AGAAACAGCAGAATAATCTA

Downstream 100 bases:

>100_bases
TGTCTGAAACCAAATTCGTACACCTTAGGGTACACAGTGATTTCTCCTTAATTGATGGGCTTTCAAAGGTTCCACCATTA
GTTAAAAAGATGGCTGAGTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTKKIDSKELPPFEYPQGYQLFAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLTDSKKLTEKKRDLLFPEIQEKALAWSL
GRCEAHEIDELNILQATMVAMQRAIAGLKVTPDFALIDGNKVPELPMAGLAVVKGDLRVQEISAASILAKVTRDREMEVL
DKEFPQYGFAKHKGYPTKAHFAAIEEHGVISEHRRSFKPVKRVLGIE

Sequences:

>Translated_207_residues
MTKKIDSKELPPFEYPQGYQLFAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLTDSKKLTEKKRDLLFPEIQEKALAWSL
GRCEAHEIDELNILQATMVAMQRAIAGLKVTPDFALIDGNKVPELPMAGLAVVKGDLRVQEISAASILAKVTRDREMEVL
DKEFPQYGFAKHKGYPTKAHFAAIEEHGVISEHRRSFKPVKRVLGIE
>Mature_206_residues
TKKIDSKELPPFEYPQGYQLFAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLTDSKKLTEKKRDLLFPEIQEKALAWSLG
RCEAHEIDELNILQATMVAMQRAIAGLKVTPDFALIDGNKVPELPMAGLAVVKGDLRVQEISAASILAKVTRDREMEVLD
KEFPQYGFAKHKGYPTKAHFAAIEEHGVISEHRRSFKPVKRVLGIE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=194, Percent_Identity=71.1340206185567, Blast_Score=280, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ALISL (B6EJW6)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263767.1
- GeneID:   6988413
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2413
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22870; Mature: 22739

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIDSKELPPFEYPQGYQLFAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLTDSKKLT
CCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHH
EKKRDLLFPEIQEKALAWSLGRCEAHEIDELNILQATMVAMQRAIAGLKVTPDFALIDGN
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEECCC
KVPELPMAGLAVVKGDLRVQEISAASILAKVTRDREMEVLDKEFPQYGFAKHKGYPTKAH
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHCCCCCCHHH
FAAIEEHGVISEHRRSFKPVKRVLGIE
HHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKIDSKELPPFEYPQGYQLFAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLTDSKKLT
CCCCCCCCCCCCCCCCCCEEEECHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHH
EKKRDLLFPEIQEKALAWSLGRCEAHEIDELNILQATMVAMQRAIAGLKVTPDFALIDGN
HHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEECCC
KVPELPMAGLAVVKGDLRVQEISAASILAKVTRDREMEVLDKEFPQYGFAKHKGYPTKAH
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHCCCCCCHHH
FAAIEEHGVISEHRRSFKPVKRVLGIE
HHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA