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The map label for this gene is tilS [H]

Identifier: 209695834

GI number: 209695834

Start: 2577219

End: 2578550

Strand: Reverse

Name: tilS [H]

Synonym: VSAL_I2410

Alternate gene names: 209695834

Gene position: 2578550-2577219 (Counterclockwise)

Preceding gene: 209695835

Following gene: 209695833

Centisome position: 77.55

GC content: 40.09

Gene sequence:

>1332_bases
ATGCTTTCTTCTCATTTCTCTCAGACATTATCTCTTTATATTTCACCTAAATCAGAACTCGTGGTTGCATTCAGCGGCGG
TGTTGATTCTGTTGTATTGCTTCATCTAGCGGCTTATTACTCTAAGTTACATCAATTACCTTGTGTTGCTGTTTATGTAA
ATCATGGATTAAGTGAACACGCTGTTGAGTGGGAAGTTTTTTGTCAATCTTATTGCGATAGCCTTGGTATCTCGTTGTTT
ATTGAACGAGTTAACTTAGATATTGGTCCACAACAGAGCTTAGAAGAAGTTGCGCGAGAAGCACGTTACGATGTGCTTGC
AAAACACATTAATAACCATTCTTTGTTATTAACAGGGCAACACCAAGATGATCAAGTAGAAACCTTTTTCTTAGCATTGA
AAAGAGGAAGTGGTCCTGCTGGTTTATCGTCAATGCCCGTAATAGCCCCTTTTGTTACGGGACGAAAATGTCGACCATTA
TTGTCTACTTCTCGGTGTGATATTGAAGCGTATGCAAACGTGAATCAATTGAAATGGGTAGAAGATGAAAGCAATAAAGA
TACTCGCTTTGACCGAAACTTCTTACGCCACAAGGTAACCCCATTGTTGCGTCAACGTTGGCCTAGTTTTGCTTCAGCGG
TGAATCGCAGTGCACAATTATGTGCAGAACAAGAGCAGTTATTGACTGAACTACTTCAACCAAAAGTGAAGGCTTATCAA
AATACGATTCAAGGTATTTCGATTGAATCGCTTGCACTTGAATCTGAAGCGGCTTGCAAGGTTATATTAAGGTTATGGTT
AAAACAGTTTTCTATTCTTTTGCCGACCCAAGCTCAATTAGGCGTGCTATGGAACGAAGTGGCTAATGCACAGCAAGATG
CAAATCCTTGTTTAGAAATTAATTCGATCCAATTACGACGTTTTCAAGGTTACCTGTATTGCCTTCCACAATATGGCGAT
GTCTCGCAGTGGAACTCGGAATTAAAGGAAATATTAACCTTACCTGATGGGTTAGGAGATATCGAATTGAAGCAAACAGT
AGGCGGCAATGATTCTGATTTGATGCCTTTATTTCTGCGATCGCCTTTGAGCGGTGAGAGAGTGACGGTGCGTTTTGATA
GTACGGGATTAATTGCTCATCCAGAGGCAAGACAACATAGTCGTAAAATGAAAAAGTTGTATCAAGAGTATGGGATCCCT
AGTTGGCAAAGAACACGATTGCCGCTCATTTTTTATAATGACCAATTAGCGGCTGTTACTGGGTTATTTGTATGTAAAGC
ATTTTCAGGAAAAGAGTGTGAGCTGATCTGGCATAAATCAGGCTCAATATAG

Upstream 100 bases:

>100_bases
GAGTAAAGTTCGGAACTAGATTCGCGAATTTTAATAATATAGAGTAAAGTGAAAACGAGCCTTAGTGCTCGTTTTTTTCA
TTTTTAGGTTTTAATTTTAT

Downstream 100 bases:

>100_bases
ACATTCGGAATTTACTTGGCAAAATGACTAAAAAAATAAAGGAGCAGTGTAAATGAAAAAAGTAATTGGACTGGCAGTTA
TCGGCTTATTGGTATCAACG

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLSSHFSQTLSLYISPKSELVVAFSGGVDSVVLLHLAAYYSKLHQLPCVAVYVNHGLSEHAVEWEVFCQSYCDSLGISLF
IERVNLDIGPQQSLEEVAREARYDVLAKHINNHSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMPVIAPFVTGRKCRPL
LSTSRCDIEAYANVNQLKWVEDESNKDTRFDRNFLRHKVTPLLRQRWPSFASAVNRSAQLCAEQEQLLTELLQPKVKAYQ
NTIQGISIESLALESEAACKVILRLWLKQFSILLPTQAQLGVLWNEVANAQQDANPCLEINSIQLRRFQGYLYCLPQYGD
VSQWNSELKEILTLPDGLGDIELKQTVGGNDSDLMPLFLRSPLSGERVTVRFDSTGLIAHPEARQHSRKMKKLYQEYGIP
SWQRTRLPLIFYNDQLAAVTGLFVCKAFSGKECELIWHKSGSI

Sequences:

>Translated_443_residues
MLSSHFSQTLSLYISPKSELVVAFSGGVDSVVLLHLAAYYSKLHQLPCVAVYVNHGLSEHAVEWEVFCQSYCDSLGISLF
IERVNLDIGPQQSLEEVAREARYDVLAKHINNHSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMPVIAPFVTGRKCRPL
LSTSRCDIEAYANVNQLKWVEDESNKDTRFDRNFLRHKVTPLLRQRWPSFASAVNRSAQLCAEQEQLLTELLQPKVKAYQ
NTIQGISIESLALESEAACKVILRLWLKQFSILLPTQAQLGVLWNEVANAQQDANPCLEINSIQLRRFQGYLYCLPQYGD
VSQWNSELKEILTLPDGLGDIELKQTVGGNDSDLMPLFLRSPLSGERVTVRFDSTGLIAHPEARQHSRKMKKLYQEYGIP
SWQRTRLPLIFYNDQLAAVTGLFVCKAFSGKECELIWHKSGSI
>Mature_443_residues
MLSSHFSQTLSLYISPKSELVVAFSGGVDSVVLLHLAAYYSKLHQLPCVAVYVNHGLSEHAVEWEVFCQSYCDSLGISLF
IERVNLDIGPQQSLEEVAREARYDVLAKHINNHSLLLTGQHQDDQVETFFLALKRGSGPAGLSSMPVIAPFVTGRKCRPL
LSTSRCDIEAYANVNQLKWVEDESNKDTRFDRNFLRHKVTPLLRQRWPSFASAVNRSAQLCAEQEQLLTELLQPKVKAYQ
NTIQGISIESLALESEAACKVILRLWLKQFSILLPTQAQLGVLWNEVANAQQDANPCLEINSIQLRRFQGYLYCLPQYGD
VSQWNSELKEILTLPDGLGDIELKQTVGGNDSDLMPLFLRSPLSGERVTVRFDSTGLIAHPEARQHSRKMKKLYQEYGIP
SWQRTRLPLIFYNDQLAAVTGLFVCKAFSGKECELIWHKSGSI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=442, Percent_Identity=40.4977375565611, Blast_Score=301, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 49927; Mature: 49927

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSHFSQTLSLYISPKSELVVAFSGGVDSVVLLHLAAYYSKLHQLPCVAVYVNHGLSEH
CCCHHHCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHH
AVEWEVFCQSYCDSLGISLFIERVNLDIGPQQSLEEVAREARYDVLAKHINNHSLLLTGQ
HHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
HQDDQVETFFLALKRGSGPAGLSSMPVIAPFVTGRKCRPLLSTSRCDIEAYANVNQLKWV
CCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEEE
EDESNKDTRFDRNFLRHKVTPLLRQRWPSFASAVNRSAQLCAEQEQLLTELLQPKVKAYQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NTIQGISIESLALESEAACKVILRLWLKQFSILLPTQAQLGVLWNEVANAQQDANPCLEI
HHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCHHCCCCCCEEE
NSIQLRRFQGYLYCLPQYGDVSQWNSELKEILTLPDGLGDIELKQTVGGNDSDLMPLFLR
CCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCHHHHC
SPLSGERVTVRFDSTGLIAHPEARQHSRKMKKLYQEYGIPSWQRTRLPLIFYNDQLAAVT
CCCCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCHHHHH
GLFVCKAFSGKECELIWHKSGSI
HHHHHHCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MLSSHFSQTLSLYISPKSELVVAFSGGVDSVVLLHLAAYYSKLHQLPCVAVYVNHGLSEH
CCCHHHCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHH
AVEWEVFCQSYCDSLGISLFIERVNLDIGPQQSLEEVAREARYDVLAKHINNHSLLLTGQ
HHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
HQDDQVETFFLALKRGSGPAGLSSMPVIAPFVTGRKCRPLLSTSRCDIEAYANVNQLKWV
CCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCEEEE
EDESNKDTRFDRNFLRHKVTPLLRQRWPSFASAVNRSAQLCAEQEQLLTELLQPKVKAYQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NTIQGISIESLALESEAACKVILRLWLKQFSILLPTQAQLGVLWNEVANAQQDANPCLEI
HHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCHHCCCCCCEEE
NSIQLRRFQGYLYCLPQYGDVSQWNSELKEILTLPDGLGDIELKQTVGGNDSDLMPLFLR
CCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCHHHHC
SPLSGERVTVRFDSTGLIAHPEARQHSRKMKKLYQEYGIPSWQRTRLPLIFYNDQLAAVT
CCCCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCHHHHH
GLFVCKAFSGKECELIWHKSGSI
HHHHHHCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA