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The map label for this gene is aotM [H]

Identifier: 209695420

GI number: 209695420

Start: 2100542

End: 2101219

Strand: Direct

Name: aotM [H]

Synonym: VSAL_I1960

Alternate gene names: 209695420

Gene position: 2100542-2101219 (Clockwise)

Preceding gene: 209695419

Following gene: 209695423

Centisome position: 63.17

GC content: 39.23

Gene sequence:

>678_bases
ATGGATTTTTCAATTATTATTGAGAGCCTACCCGTTTATTTGGATGGCCTTTGGACTACTGTTTGGTTAGTCGCTTTATC
TCTTATTATTGGGTTGTGCATTGCCGTCCCTACCGCGATTGCTAGAAACAGCAATAATCCATTAATTTGGGTTCCTGCGT
GGGCCTATATTTATTTCTTTCGCGGTACACCATTACTGATCCAACTGTATCTGATTTATTACGGAATGGATCAGTTTTAT
CCCGTGAAAGACACGGTATGGGAACATGCATCATTTTGTGCATTGGTTGCGTTTGCATTAAATACAGGCGCTTATACTTC
GGAGATCGTTCGAGGCTCTATTAATGGCTTACCTCAAGGTGAAATTGAAGCGGCAAAAGCGTATGGCATGAGTCAATTTC
AAACTTATAAACGCATTATTTTACCGAGTGCATTACGCAGAGCGTTGCCAGCATACAGTAATGAAGTGATTTTCATGTTA
CATGGTTCTGCGATTGCAGGCATTGTAACTTTGATGGATTTGACTGGCGCAGCACGATTAGTGAATTCTCGTTACTACGC
ACCTTTTGAGTCATATATTGCGGCAGGTTTGTTTTACATGTGTCTAACGTTCATTATTATTTTTGTATTTAAACAGTTAG
AAACACGAATGCTTAGATTTATGAGACCATTAAGTTAA

Upstream 100 bases:

>100_bases
TTTATATGACCGTTGCTTGTATTTTCTTATTATTCACTGCCGTGTCGACTGGTGCTTTAAAACTGGTTGAGCGTAAATTT
AGTATTCATACGAGGTAAGC

Downstream 100 bases:

>100_bases
TTGTATTTTAAGTTATAATCAAAAAGAGCACAGAACTGTGCTCTTTTTTTTTAATGTAGTATAGCCAGATTATACTCTTT
GAGGAGGTCGTTATGGGCTT

Product: arginine/ornithine periplasmic binding protein-dependent transporter inner membrane component

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDFSIIIESLPVYLDGLWTTVWLVALSLIIGLCIAVPTAIARNSNNPLIWVPAWAYIYFFRGTPLLIQLYLIYYGMDQFY
PVKDTVWEHASFCALVAFALNTGAYTSEIVRGSINGLPQGEIEAAKAYGMSQFQTYKRIILPSALRRALPAYSNEVIFML
HGSAIAGIVTLMDLTGAARLVNSRYYAPFESYIAAGLFYMCLTFIIIFVFKQLETRMLRFMRPLS

Sequences:

>Translated_225_residues
MDFSIIIESLPVYLDGLWTTVWLVALSLIIGLCIAVPTAIARNSNNPLIWVPAWAYIYFFRGTPLLIQLYLIYYGMDQFY
PVKDTVWEHASFCALVAFALNTGAYTSEIVRGSINGLPQGEIEAAKAYGMSQFQTYKRIILPSALRRALPAYSNEVIFML
HGSAIAGIVTLMDLTGAARLVNSRYYAPFESYIAAGLFYMCLTFIIIFVFKQLETRMLRFMRPLS
>Mature_225_residues
MDFSIIIESLPVYLDGLWTTVWLVALSLIIGLCIAVPTAIARNSNNPLIWVPAWAYIYFFRGTPLLIQLYLIYYGMDQFY
PVKDTVWEHASFCALVAFALNTGAYTSEIVRGSINGLPQGEIEAAKAYGMSQFQTYKRIILPSALRRALPAYSNEVIFML
HGSAIAGIVTLMDLTGAARLVNSRYYAPFESYIAAGLFYMCLTFIIIFVFKQLETRMLRFMRPLS

Specific function: Component of the nopaline active transport system probably consisting of four subunits:Q, M, P and T. This system is also capable of transporting octopine provided that catabolic functions are induced with nopaline [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=215, Percent_Identity=46.5116279069767, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1787086, Length=221, Percent_Identity=35.7466063348416, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1788226, Length=224, Percent_Identity=34.375, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787030, Length=216, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787087, Length=223, Percent_Identity=26.9058295964126, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786873, Length=210, Percent_Identity=32.3809523809524, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788646, Length=214, Percent_Identity=29.4392523364486, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786874, Length=233, Percent_Identity=26.6094420600858, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI87082239, Length=203, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25392; Mature: 25392

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSIIIESLPVYLDGLWTTVWLVALSLIIGLCIAVPTAIARNSNNPLIWVPAWAYIYFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
RGTPLLIQLYLIYYGMDQFYPVKDTVWEHASFCALVAFALNTGAYTSEIVRGSINGLPQG
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
EIEAAKAYGMSQFQTYKRIILPSALRRALPAYSNEVIFMLHGSAIAGIVTLMDLTGAARL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
VNSRYYAPFESYIAAGLFYMCLTFIIIFVFKQLETRMLRFMRPLS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFSIIIESLPVYLDGLWTTVWLVALSLIIGLCIAVPTAIARNSNNPLIWVPAWAYIYFF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
RGTPLLIQLYLIYYGMDQFYPVKDTVWEHASFCALVAFALNTGAYTSEIVRGSINGLPQG
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
EIEAAKAYGMSQFQTYKRIILPSALRRALPAYSNEVIFMLHGSAIAGIVTLMDLTGAARL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
VNSRYYAPFESYIAAGLFYMCLTFIIIFVFKQLETRMLRFMRPLS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1732218; 11743193; 11743194 [H]