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The map label for this gene is aotJ [H]

Identifier: 209695418

GI number: 209695418

Start: 2098933

End: 2099709

Strand: Direct

Name: aotJ [H]

Synonym: VSAL_I1958

Alternate gene names: 209695418

Gene position: 2098933-2099709 (Clockwise)

Preceding gene: 209695417

Following gene: 209695419

Centisome position: 63.12

GC content: 39.38

Gene sequence:

>777_bases
ATGAAGAAGTGGTTATTAGCAGCAATTGTTGCATCTACAGCCGTTACTGGCGCAGCTCAAGCAAAAGAATGGAAGCAAAT
TCGATTTGGTATTGAAGGGGCATATCCTCCATTTAGTTGGACTGAACCATCAGGTGAACTGAAAGGGTTTGATGTCGATA
TCGCTAACGCACTATGTAAAGAGATGGAAGCTCGTTGTAAGATCGTTGCTCAAGATTGGGATGGTATTATCCCATCACTA
TTGGCTCGTAAATATGATGCGATTATTGCAGCAATGTCTATTACAGAAGAGCGCAAGAAAAAAATCGATTTCACTGAGAA
ATACGCACTGATCCCGAATAAATTTGTTGCTAAAAAAGGCACAGAATTAGTCATGACTAAAGAAGGTCTAAAAGGGGTAA
CCGTTGGTGTTCAACGCGCGACAACACACGATAAATATTTGACAGACAACTTTGGTTCTGGTGTTGAGCTTAAGCGTTAT
GGCTCATTTGATGAAGCATATCTAGATCTTAAAGCGGGTCGTATTGATACTGTTCTTGGTGATGCATCAGCATTAGAAGA
AGGGTTACTAAACAAACCTGGCGGTAAAGAATACGAATTTGTTGGTCCTTCGCTAACGGATCCAAAATGGTTTGGTGATG
GGTTTGGTATCGCAACTCGCAAACAAGATAAAGATTTAACAAAACAATTGAATGCCGCAATTGATTCACTTCGTGAAAAA
GGTGTTTATCAAGAAATTGCAGCAAAATACTTCAACTATGATGTATATGGTGAATAA

Upstream 100 bases:

>100_bases
TTCGTCAATATACTGATTGGTTTATTGATGTTTTAAGCAATACCGAAATAACGCAGTGTATTAAATCAATAAAAATGAAG
TTAGTTACAGGAGCAAGGAA

Downstream 100 bases:

>100_bases
GTACAAAAAGTACTAATTCATATGAAAGAGGCCGTAGTCTATAGCCTCTTTCAATTTTCTAATAGTTAAAGCGAAAGCTT
GGAAGCAGTATGTTAGATTT

Product: arginine/ornithine periplasmic binding protein-dependent transporter

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKWLLAAIVASTAVTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCKEMEARCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKGTELVMTKEGLKGVTVGVQRATTHDKYLTDNFGSGVELKRY
GSFDEAYLDLKAGRIDTVLGDASALEEGLLNKPGGKEYEFVGPSLTDPKWFGDGFGIATRKQDKDLTKQLNAAIDSLREK
GVYQEIAAKYFNYDVYGE

Sequences:

>Translated_258_residues
MKKWLLAAIVASTAVTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCKEMEARCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKGTELVMTKEGLKGVTVGVQRATTHDKYLTDNFGSGVELKRY
GSFDEAYLDLKAGRIDTVLGDASALEEGLLNKPGGKEYEFVGPSLTDPKWFGDGFGIATRKQDKDLTKQLNAAIDSLREK
GVYQEIAAKYFNYDVYGE
>Mature_258_residues
MKKWLLAAIVASTAVTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCKEMEARCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKGTELVMTKEGLKGVTVGVQRATTHDKYLTDNFGSGVELKRY
GSFDEAYLDLKAGRIDTVLGDASALEEGLLNKPGGKEYEFVGPSLTDPKWFGDGFGIATRKQDKDLTKQLNAAIDSLREK
GVYQEIAAKYFNYDVYGE

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=40, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=42.3076923076923, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=41.4342629482072, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=39.0438247011952, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=32.9457364341085, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787031, Length=251, Percent_Identity=29.8804780876494, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28560; Mature: 28560

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLAAIVASTAVTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EMEARCKIVAQDWDGIIPSLLARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKG
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCC
TELVMTKEGLKGVTVGVQRATTHDKYLTDNFGSGVELKRYGSFDEAYLDLKAGRIDTVLG
CEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCCCEEHCCCCHHHHHHHHCCCCHHHHHC
DASALEEGLLNKPGGKEYEFVGPSLTDPKWFGDGFGIATRKQDKDLTKQLNAAIDSLREK
CHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GVYQEIAAKYFNYDVYGE
CHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKWLLAAIVASTAVTGAAQAKEWKQIRFGIEGAYPPFSWTEPSGELKGFDVDIANALCK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EMEARCKIVAQDWDGIIPSLLARKYDAIIAAMSITEERKKKIDFTEKYALIPNKFVAKKG
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCC
TELVMTKEGLKGVTVGVQRATTHDKYLTDNFGSGVELKRYGSFDEAYLDLKAGRIDTVLG
CEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCCCEEHCCCCHHHHHHHHCCCCHHHHHC
DASALEEGLLNKPGGKEYEFVGPSLTDPKWFGDGFGIATRKQDKDLTKQLNAAIDSLREK
CHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GVYQEIAAKYFNYDVYGE
CHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]