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The map label for this gene is purT [H]

Identifier: 209695180

GI number: 209695180

Start: 1781538

End: 1782713

Strand: Direct

Name: purT [H]

Synonym: VSAL_I1686

Alternate gene names: 209695180

Gene position: 1781538-1782713 (Clockwise)

Preceding gene: 209695178

Following gene: 209695181

Centisome position: 53.58

GC content: 42.94

Gene sequence:

>1176_bases
ATGTTTGGTACTGCTCTATCGTCTTCAGCTACAAAAGTATTATTGCTTGGTTCCGGTGAATTAGGCAAAGAAGTGGCTAT
TGAATGCCAACGCCTTGGTTTGGAAGTTATTGCGGTTGATCGCTATTTAAATGCCCCAGCAATGCAAGTCGCACACCGTA
GCTATGCCATTGATATGTTAGATGCTGATGCACTTCAAGAAGTCATTGAAAAAGAAAAACCAGATTATGTCGTGCCTGAA
ATCGAAGCCATTGCCACAGACAAACTCGTTGAATTAGAAAAACGAGGCCTTAACGTTGTTCCAACCGCTAACGCGACTAA
ATTGACCATGAACCGCGAAGGCATTCGTCGATTAGCCGCTGAAGAATTAAAATTACCGACGTCTCCTTATCGTTTTGTAG
ACAGCTATGAAGAACTGGTCGACGCCGTTAAATTTGTAGGTATGCCGTGTGTAATTAAGCCGGTAATGAGTTCATCAGGC
AAAGGACAAAGCGTCATCAAGACCGAGGATGATATTAAAACCTCATGGGACTACGCGCAAGATGGCGGACGAACGGGAGC
AGGTCGCGTTATTGTTGAAGGTTTTGTGGATTTTGATTACGAAATTTCACTGTTAACTATCCGTGCGGTTGATGGCATTC
ATTTCTGTGATCCTATCGGTCATCGACAAGAAGATGGTGATTACCGCGAGTCTTGGCAACCTCAAGTAATGACGGAAGCG
GCATTACAACAAGCACAAGACGCGGCAGAAAAAGTCGTTAACGCATTAGGCGGTTATGGTTTATTTGGTGTAGAGCTCTT
CATTAAAGGCGATGATATTTATTTCAATGAAGTCTCACCTCGCCCACATGATACTGGATTAGTAACGTTAATCTCTCAAG
AAATGTCTGAATTTGCTCTGCATGTTCGTGCGTTTACGGGAATGCCCATCAATAACATCGTTCAATATGGTCCTTCTGCC
TCTTCTGTGATCTTAGGTCAAGGTACCTCTACAAATATTCGTTTTGATGGACTGGCAGAGGCATTGAGCCAACCACAAAC
TCAAATAAAATTATTTGGTAAACCTGAAATTGATGGGCGTCGTCGCTTAGGGGTCGCCATAACACGCAGAGACAATCTTG
AGTCAGCAATTGAAGATGCTATCGCAGCGTCAAACAAAATTAAAATCAATTACTAA

Upstream 100 bases:

>100_bases
TGCATAAAAGACCAATAAAAAATTAAAAGCAATCGTTTGCGTATCTATCTAATTTTAGTATGATAGCGGCATATTTCACT
ATCTATCATGAGAATTCATT

Downstream 100 bases:

>100_bases
CCTTGCTTTCATTCAGCAGAACGGTATTTAAAATGCCGTTCTGTTGTCATTTATTACCATTCTGCCTCTCTTTTTCCTTT
TTACGATTAAAAATCACTTT

Product: phosphoribosylglycinamide formyltransferase 2

Products: tetrahydrofolate; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGLEVIAVDRYLNAPAMQVAHRSYAIDMLDADALQEVIEKEKPDYVVPE
IEAIATDKLVELEKRGLNVVPTANATKLTMNREGIRRLAAEELKLPTSPYRFVDSYEELVDAVKFVGMPCVIKPVMSSSG
KGQSVIKTEDDIKTSWDYAQDGGRTGAGRVIVEGFVDFDYEISLLTIRAVDGIHFCDPIGHRQEDGDYRESWQPQVMTEA
ALQQAQDAAEKVVNALGGYGLFGVELFIKGDDIYFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPINNIVQYGPSA
SSVILGQGTSTNIRFDGLAEALSQPQTQIKLFGKPEIDGRRRLGVAITRRDNLESAIEDAIAASNKIKINY

Sequences:

>Translated_391_residues
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGLEVIAVDRYLNAPAMQVAHRSYAIDMLDADALQEVIEKEKPDYVVPE
IEAIATDKLVELEKRGLNVVPTANATKLTMNREGIRRLAAEELKLPTSPYRFVDSYEELVDAVKFVGMPCVIKPVMSSSG
KGQSVIKTEDDIKTSWDYAQDGGRTGAGRVIVEGFVDFDYEISLLTIRAVDGIHFCDPIGHRQEDGDYRESWQPQVMTEA
ALQQAQDAAEKVVNALGGYGLFGVELFIKGDDIYFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPINNIVQYGPSA
SSVILGQGTSTNIRFDGLAEALSQPQTQIKLFGKPEIDGRRRLGVAITRRDNLESAIEDAIAASNKIKINY
>Mature_391_residues
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGLEVIAVDRYLNAPAMQVAHRSYAIDMLDADALQEVIEKEKPDYVVPE
IEAIATDKLVELEKRGLNVVPTANATKLTMNREGIRRLAAEELKLPTSPYRFVDSYEELVDAVKFVGMPCVIKPVMSSSG
KGQSVIKTEDDIKTSWDYAQDGGRTGAGRVIVEGFVDFDYEISLLTIRAVDGIHFCDPIGHRQEDGDYRESWQPQVMTEA
ALQQAQDAAEKVVNALGGYGLFGVELFIKGDDIYFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPINNIVQYGPSA
SSVILGQGTSTNIRFDGLAEALSQPQTQIKLFGKPEIDGRRRLGVAITRRDNLESAIEDAIAASNKIKINY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI170295797, Length=207, Percent_Identity=25.1207729468599, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI169790915, Length=207, Percent_Identity=25.1207729468599, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI21361331, Length=207, Percent_Identity=25.1207729468599, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788155, Length=390, Percent_Identity=67.1794871794872, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1786733, Length=365, Percent_Identity=22.4657534246575, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=24.2506811989101, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.2

Molecular weight: Translated: 42833; Mature: 42833

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGLEVIAVDRYLNAPAMQVAHRSYAIDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHEEECC
DADALQEVIEKEKPDYVVPEIEAIATDKLVELEKRGLNVVPTANATKLTMNREGIRRLAA
CHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECHHHHHHHHH
EELKLPTSPYRFVDSYEELVDAVKFVGMPCVIKPVMSSSGKGQSVIKTEDDIKTSWDYAQ
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEECCCCCCCCCCHHH
DGGRTGAGRVIVEGFVDFDYEISLLTIRAVDGIHFCDPIGHRQEDGDYRESWQPQVMTEA
CCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
ALQQAQDAAEKVVNALGGYGLFGVELFIKGDDIYFNEVSPRPHDTGLVTLISQEMSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HVRAFTGMPINNIVQYGPSASSVILGQGTSTNIRFDGLAEALSQPQTQIKLFGKPEIDGR
HHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCEEEHHHHHHHCCCCEEEEEECCCCCCCC
RRLGVAITRRDNLESAIEDAIAASNKIKINY
CEEEEEEEECCCHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGLEVIAVDRYLNAPAMQVAHRSYAIDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHEEECC
DADALQEVIEKEKPDYVVPEIEAIATDKLVELEKRGLNVVPTANATKLTMNREGIRRLAA
CHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECHHHHHHHHH
EELKLPTSPYRFVDSYEELVDAVKFVGMPCVIKPVMSSSGKGQSVIKTEDDIKTSWDYAQ
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEECCCCCCCCCCHHH
DGGRTGAGRVIVEGFVDFDYEISLLTIRAVDGIHFCDPIGHRQEDGDYRESWQPQVMTEA
CCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
ALQQAQDAAEKVVNALGGYGLFGVELFIKGDDIYFNEVSPRPHDTGLVTLISQEMSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HVRAFTGMPINNIVQYGPSASSVILGQGTSTNIRFDGLAEALSQPQTQIKLFGKPEIDGR
HHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCEEEHHHHHHHCCCCEEEEEECCCCCCCC
RRLGVAITRRDNLESAIEDAIAASNKIKINY
CEEEEEEEECCCHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 10-formyltetrahydrofolate; N1-(5-phospho-D-ribosyl)glycinamide

Specific reaction: 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA