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The map label for this gene is znuB [H]

Identifier: 209694429

GI number: 209694429

Start: 946963

End: 947748

Strand: Reverse

Name: znuB [H]

Synonym: VSAL_I0850

Alternate gene names: 209694429

Gene position: 947748-946963 (Counterclockwise)

Preceding gene: 209694430

Following gene: 209694420

Centisome position: 28.5

GC content: 39.19

Gene sequence:

>786_bases
ATGATTGAGTTTTTGCTTCCTGCTCTTTTAGCAGGATTAGGCATTGCATTACTTGCAGGGCCATTAGGTTCTTTTGTTGT
ATGGAGAAAAATGGCCTATTTTGGCGATACTCTCGCTCATGCCTCTTTACTTGGGTTATCTCTTGGCTTTTTATTCGACA
TCAACTTAAATTTAGCGTTGATCGTCTGCTGTATTGCCATCGCATTATTATTAGTTACGCTACAAAAACAACGTTTAGTC
GCAACTGATACCTTACTTGGTATTTTAGCGCATAGCTCTCTCTCGCTAGGACTCGTTGCTGTCAGCTTTTTAGATAATGT
TCGAGTCGACTTAATGTCTTATTTATTCGGTGATTTACTTGCCGTAACTTATAACGATTTATTGTATATCTATACTGGTG
TGATTATCGTTTTAGGCCAGATATACATTGTCTGGAGACCATTATTATCATCTACAATTAGTGAAGAATTAGCTCAAGTT
GAAGGGGTTAACGTTGAATTAATGCGCTTGGTCTTAATGTTAATGGTTGGGTTGGTTATTGCGATATCAATGAAGTTTGT
TGGTGCATTAATTATTACTTCGTTGCTAATTATTCCTGCGGCTACCGCTCGACGTTTTTCTCGTTCACCAGAACAAATGG
TGCTATTCGCATCAATCTTTGGTTGTATTGCCGTTATTGCAGGTCTTGCCCTTTCTTGGCACTACGATACCCCAGCGGGC
CCTTCCGTTGTTGTTTCTGGCGCTGCTATCTTTATGTTATCTCAGTTAAAGAAAACGACTCAATAA

Upstream 100 bases:

>100_bases
AAGAATTGGCCTTCTACCATCATGACCACCACCACAATCATGATCTTTCTGGTGAACCAACAAATGGTTCATGCTGCAGT
AAAAATAAAAAGGCATCATC

Downstream 100 bases:

>100_bases
CAGTCTTAATATAATAATAAGCTTTAGATAATACCTGTAAATAAAAACCCCGAGCTCACAAGAACTCGGGGTTTATTTTT
ACGGGAATATCAGCAGTTGA

Product: high-affinity zinc uptake system membrane protein ZnuB

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIEFLLPALLAGLGIALLAGPLGSFVVWRKMAYFGDTLAHASLLGLSLGFLFDINLNLALIVCCIAIALLLVTLQKQRLV
ATDTLLGILAHSSLSLGLVAVSFLDNVRVDLMSYLFGDLLAVTYNDLLYIYTGVIIVLGQIYIVWRPLLSSTISEELAQV
EGVNVELMRLVLMLMVGLVIAISMKFVGALIITSLLIIPAATARRFSRSPEQMVLFASIFGCIAVIAGLALSWHYDTPAG
PSVVVSGAAIFMLSQLKKTTQ

Sequences:

>Translated_261_residues
MIEFLLPALLAGLGIALLAGPLGSFVVWRKMAYFGDTLAHASLLGLSLGFLFDINLNLALIVCCIAIALLLVTLQKQRLV
ATDTLLGILAHSSLSLGLVAVSFLDNVRVDLMSYLFGDLLAVTYNDLLYIYTGVIIVLGQIYIVWRPLLSSTISEELAQV
EGVNVELMRLVLMLMVGLVIAISMKFVGALIITSLLIIPAATARRFSRSPEQMVLFASIFGCIAVIAGLALSWHYDTPAG
PSVVVSGAAIFMLSQLKKTTQ
>Mature_261_residues
MIEFLLPALLAGLGIALLAGPLGSFVVWRKMAYFGDTLAHASLLGLSLGFLFDINLNLALIVCCIAIALLLVTLQKQRLV
ATDTLLGILAHSSLSLGLVAVSFLDNVRVDLMSYLFGDLLAVTYNDLLYIYTGVIIVLGQIYIVWRPLLSSTISEELAQV
EGVNVELMRLVLMLMVGLVIAISMKFVGALIITSLLIIPAATARRFSRSPEQMVLFASIFGCIAVIAGLALSWHYDTPAG
PSVVVSGAAIFMLSQLKKTTQ

Specific function: Involved in the high-affinity zinc uptake transport system [H]

COG id: COG1108

COG function: function code P; ABC-type Mn2+/Zn2+ transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC-3 integral membrane protein family [H]

Homologues:

Organism=Escherichia coli, GI1788166, Length=258, Percent_Identity=60.4651162790698, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001626 [H]

Pfam domain/function: PF00950 ABC-3 [H]

EC number: NA

Molecular weight: Translated: 28043; Mature: 28043

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFLLPALLAGLGIALLAGPLGSFVVWRKMAYFGDTLAHASLLGLSLGFLFDINLNLAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHH
IVCCIAIALLLVTLQKQRLVATDTLLGILAHSSLSLGLVAVSFLDNVRVDLMSYLFGDLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTYNDLLYIYTGVIIVLGQIYIVWRPLLSSTISEELAQVEGVNVELMRLVLMLMVGLVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AISMKFVGALIITSLLIIPAATARRFSRSPEQMVLFASIFGCIAVIAGLALSWHYDTPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
PSVVVSGAAIFMLSQLKKTTQ
CHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEFLLPALLAGLGIALLAGPLGSFVVWRKMAYFGDTLAHASLLGLSLGFLFDINLNLAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHH
IVCCIAIALLLVTLQKQRLVATDTLLGILAHSSLSLGLVAVSFLDNVRVDLMSYLFGDLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTYNDLLYIYTGVIIVLGQIYIVWRPLLSSTISEELAQVEGVNVELMRLVLMLMVGLVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AISMKFVGALIITSLLIIPAATARRFSRSPEQMVLFASIFGCIAVIAGLALSWHYDTPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
PSVVVSGAAIFMLSQLKKTTQ
CHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 2842314; 7984428; 9680209 [H]