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The map label for this gene is rimK

Identifier: 209694348

GI number: 209694348

Start: 849441

End: 850346

Strand: Reverse

Name: rimK

Synonym: VSAL_I0760

Alternate gene names: 209694348

Gene position: 850346-849441 (Counterclockwise)

Preceding gene: 209694349

Following gene: 209694347

Centisome position: 25.57

GC content: 41.72

Gene sequence:

>906_bases
ATGAAAATCGGTATTTTATCTCGTAACCAAGACCTTTACTCTACTCGCCGTTTAATTGAAGCGGCGGAAAGCCGTGGTCA
TGAAGTAAAAGTAATTGATGCATTACGCTGTTATATGAATATCAATTCAGAAAAACCTCAAATTCACTTCAAAGGTGAAG
AACTGATTAATTACGATGCGATTATCCCTCGTATTGGCGCATCAGTTACTTTTTATGGTACTGCTGTATTACGTCAATTT
GAAATGATGAATGTCTACCCGGTGAACGAATCTGTTGCTATCACTCGTTCTCGCGATAAATTACGTTCAATGCAACTGCT
TTCTCGTAAAGGCATTGGTATGCCAATCACGGGTTTTGCAAGCAAGCCTGATGATGTAAAAGATTTACTTGATATGGTTG
GCGGCGGGCCAGTTGTGATCAAGCTCTTAGAAGGCACTCAAGGTATTGGTGTTGTTCTTGCAGAAACAAGAAAAGCAGCA
GAAAGTGTTGTTGAAGCATTTATGGGCTTAAAAGCGAATATTATGGTTCAAGAGTTCATCAAAGAAGCTGGTGGTGCAGA
TATCCGTTGTTTCGTTATTGGTGGCAAAGTGATTGCAGCAATGAAACGTCAAGGTGCAGAAGGCGAATTCCGTTCTAACC
TACACCGTGGTGGTTCTGCGTCACTGGTTAAACTGACTCCAGAAGAACGTAAAACAGCAGTAGCAGCAGCAAATATCATG
GGATTGAACGTAGCAGGTGTCGATTTACTTCGCTCTGATCGTGGTCCATTAGTTATGGAAGTGAACTCTTCACCTGGCTT
AGAAGGCATTGAAGCCGCTACAGGTAAAGACGTTGCTGGTTTAATTGTTGATTTTATTGAGAAAAATGCAGCGACAAAAG
GGACTAAAACTCGTGGCAAAGGCTAG

Upstream 100 bases:

>100_bases
CAATGGTGTTTCGTATGTTACTTGGCAGATCATCAATGCAAGACCGCATTGTTGTTGATCCATCGTCGTCTTACCTAGTT
GATTTTGAGGAAATTGTATA

Downstream 100 bases:

>100_bases
ACAGAACAAAGCATTCGACATACTAACGTATTCAGTTGCACCCGGTGAAAGAAAGGTCATTGAGATTGAAGCCGCAAAGC
TTTATACCCACTCACCTTTA

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIGILSRNQDLYSTRRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELINYDAIIPRIGASVTFYGTAVLRQF
EMMNVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFASKPDDVKDLLDMVGGGPVVIKLLEGTQGIGVVLAETRKAA
ESVVEAFMGLKANIMVQEFIKEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEAATGKDVAGLIVDFIEKNAATKGTKTRGKG

Sequences:

>Translated_301_residues
MKIGILSRNQDLYSTRRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELINYDAIIPRIGASVTFYGTAVLRQF
EMMNVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFASKPDDVKDLLDMVGGGPVVIKLLEGTQGIGVVLAETRKAA
ESVVEAFMGLKANIMVQEFIKEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEAATGKDVAGLIVDFIEKNAATKGTKTRGKG
>Mature_301_residues
MKIGILSRNQDLYSTRRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELINYDAIIPRIGASVTFYGTAVLRQF
EMMNVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFASKPDDVKDLLDMVGGGPVVIKLLEGTQGIGVVLAETRKAA
ESVVEAFMGLKANIMVQEFIKEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEAATGKDVAGLIVDFIEKNAATKGTKTRGKG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=214, Percent_Identity=31.3084112149533, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI134254462, Length=214, Percent_Identity=30.3738317757009, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787076, Length=293, Percent_Identity=64.5051194539249, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_ALISL (B6EH18)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262276.1
- GeneID:   6986643
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0760
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- ProtClustDB:   CLSK794626
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32391; Mature: 32391

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILSRNQDLYSTRRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELINYDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCEEEECCHHHCCHHH
IIPRIGASVTFYGTAVLRQFEMMNVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFA
HHHHCCCEEEEHHHHHHHHHHHCEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
SKPDDVKDLLDMVGGGPVVIKLLEGTQGIGVVLAETRKAAESVVEAFMGLKANIMVQEFI
CCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHH
KEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
HHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHEE
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEAATGKDVAGLIVDFIEKNAATKGTKTRGK
ECEECCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MKIGILSRNQDLYSTRRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELINYDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCEEEECCHHHCCHHH
IIPRIGASVTFYGTAVLRQFEMMNVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFA
HHHHCCCEEEEHHHHHHHHHHHCEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
SKPDDVKDLLDMVGGGPVVIKLLEGTQGIGVVLAETRKAAESVVEAFMGLKANIMVQEFI
CCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHH
KEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
HHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHEE
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEAATGKDVAGLIVDFIEKNAATKGTKTRGK
ECEECCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA