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The map label for this gene is mltF [H]

Identifier: 209694337

GI number: 209694337

Start: 835973

End: 837457

Strand: Reverse

Name: mltF [H]

Synonym: VSAL_I0749

Alternate gene names: 209694337

Gene position: 837457-835973 (Counterclockwise)

Preceding gene: 209694341

Following gene: 209694334

Centisome position: 25.19

GC content: 38.99

Gene sequence:

>1485_bases
TTGAGCCACATCCGATATTATCGTCTTCTAAAATCTTGCCTGGTTATTTCTACGCTTGTATTTACCTTAACGGGTTGCCA
AGTAGAATCAGAACCAAAAACAAAACTAGAGCAAGTAAGAGAACGTGGCATCTTAAGAGTCAGTACATTAAACAATCAAC
TCTCTTATTATATTGGCTCGAACGGTCCTACAGGGCTTGATTATGATCTTGCGAAAGCCTTTGCTGAAAAGCTAAATGTA
AAACTAGAAATCACGCCTGCTTATACGTATTCAGGTTTATTCCCAGCGCTAAACCGCAACGAACTCGATATTGTTGCCGC
TAATATGACCATTACCCCTGAACGTCTAGAAAACTTTCGTACCGGACCAATTTATTATTATGTTAGTCAACAGGTCGTGT
ATAAAAAAGGGAACTGGCGCCCTCGAAATATCAAAAACCTCACAGAATTAGATGAAAACTTAACGATTGTGAAAGATTCT
AGTTTTGAAGGCACTTTATTAAAATTAAAAGAAAAATATAAAAAATTAGAATGGTCTACAGAAGATAATACGGATGTAAG
TGAACTATTAAAACAAGTCGCACGCGGTGAAATACATTACACCTTAGCCGATTCGGTTGAACTGTCTCTTACCCAACGAA
TTCATCCCGATCTCGCTGTCGCTTTTGAGTTAACCGAAGATCAACCTATCGCTTGGTTCTTACAAAAAACAGAAGACGAT
AGCCTTCAGGCATTATTGATTGAGTTTTTTGGTGAGCTAAAAAGCAGTGGTAAATTAGCATTACTAGAAGAAAAATATTT
TGGTCACGTAGAAACGTTCGATTACGTTGATACACGTGCTTTCATTCGTTCACTAGAAAGTAAGTTACCGAAATGGGAAC
CTTTATTTAAAAAATACGCCGGGGATTTTGATTGGCGTTTCTTAGCCGCTCTCTCATACCAAGAATCACATTGGAACCCT
GTTGCTAAATCTCCTACAGGTGTGCGTGGCATGATGATGCTCACCTTACCAACGGCTCAATCTGTTGGGGTTAAAAATCG
ATTAGATCCAGAAGAAAGCATTCGCGGGGGAGCTGAGTATTTACGTCGTATAGTAAAACGTGTACCAGATTCAATTACCG
AACATGAGAAAATTTGGTTTGCCTTAGCGTCTTATAACATTGGTTTCGGACACATGATGGACGCCCGTCGTTTAACACAA
CGATTAGGGGGGGATCCTAACTCATGGACAGACGTAAAAGACAATTTGCCTTTGTTACGCCAGAAACGTTATTACAAATA
CCTTCGCTATGGATTTGCTCGTGGTGATGAAGCTCAAAACTACGTTGAAAATATCCGTCGTTATTACCAAAGTATTATTG
GTTATGAACAAGAACAAGCAAACAAATTAATCCAAGAAGATATCACTGTTGAAGATTTAACTGTCATTGATGTTCCATTG
TCATCAGCAACGTCTACGACAAGCCACGCAGTTTCAAGTCAGTAA

Upstream 100 bases:

>100_bases
CTTAATCTCTCTTTATTGTTCTAATTTGTTACTGCGCTTTTGCCTCTTTTTCTCCCATTTGATATAACTACGCCCTACTT
ATTTAAAAGAGCATTCTGAC

Downstream 100 bases:

>100_bases
TTTGTTACTGTATAAAAAAGGCATTAGGATGATAACGGCTGACTCGATGAGAACGTTCATATAACGGTAACAATTACCAC
ATACTCTGAGCTAAATAGAT

Product: transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSHIRYYRLLKSCLVISTLVFTLTGCQVESEPKTKLEQVRERGILRVSTLNNQLSYYIGSNGPTGLDYDLAKAFAEKLNV
KLEITPAYTYSGLFPALNRNELDIVAANMTITPERLENFRTGPIYYYVSQQVVYKKGNWRPRNIKNLTELDENLTIVKDS
SFEGTLLKLKEKYKKLEWSTEDNTDVSELLKQVARGEIHYTLADSVELSLTQRIHPDLAVAFELTEDQPIAWFLQKTEDD
SLQALLIEFFGELKSSGKLALLEEKYFGHVETFDYVDTRAFIRSLESKLPKWEPLFKKYAGDFDWRFLAALSYQESHWNP
VAKSPTGVRGMMMLTLPTAQSVGVKNRLDPEESIRGGAEYLRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQ
RLGGDPNSWTDVKDNLPLLRQKRYYKYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLIQEDITVEDLTVIDVPL
SSATSTTSHAVSSQ

Sequences:

>Translated_494_residues
MSHIRYYRLLKSCLVISTLVFTLTGCQVESEPKTKLEQVRERGILRVSTLNNQLSYYIGSNGPTGLDYDLAKAFAEKLNV
KLEITPAYTYSGLFPALNRNELDIVAANMTITPERLENFRTGPIYYYVSQQVVYKKGNWRPRNIKNLTELDENLTIVKDS
SFEGTLLKLKEKYKKLEWSTEDNTDVSELLKQVARGEIHYTLADSVELSLTQRIHPDLAVAFELTEDQPIAWFLQKTEDD
SLQALLIEFFGELKSSGKLALLEEKYFGHVETFDYVDTRAFIRSLESKLPKWEPLFKKYAGDFDWRFLAALSYQESHWNP
VAKSPTGVRGMMMLTLPTAQSVGVKNRLDPEESIRGGAEYLRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQ
RLGGDPNSWTDVKDNLPLLRQKRYYKYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLIQEDITVEDLTVIDVPL
SSATSTTSHAVSSQ
>Mature_493_residues
SHIRYYRLLKSCLVISTLVFTLTGCQVESEPKTKLEQVRERGILRVSTLNNQLSYYIGSNGPTGLDYDLAKAFAEKLNVK
LEITPAYTYSGLFPALNRNELDIVAANMTITPERLENFRTGPIYYYVSQQVVYKKGNWRPRNIKNLTELDENLTIVKDSS
FEGTLLKLKEKYKKLEWSTEDNTDVSELLKQVARGEIHYTLADSVELSLTQRIHPDLAVAFELTEDQPIAWFLQKTEDDS
LQALLIEFFGELKSSGKLALLEEKYFGHVETFDYVDTRAFIRSLESKLPKWEPLFKKYAGDFDWRFLAALSYQESHWNPV
AKSPTGVRGMMMLTLPTAQSVGVKNRLDPEESIRGGAEYLRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQR
LGGDPNSWTDVKDNLPLLRQKRYYKYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLIQEDITVEDLTVIDVPLS
SATSTTSHAVSSQ

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=426, Percent_Identity=50.7042253521127, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 56842; Mature: 56711

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIRYYRLLKSCLVISTLVFTLTGCQVESEPKTKLEQVRERGILRVSTLNNQLSYYIGS
CCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHCCCEEEEEECCCEEEEECC
NGPTGLDYDLAKAFAEKLNVKLEITPAYTYSGLFPALNRNELDIVAANMTITPERLENFR
CCCCCCCHHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCEEEEEECCEECHHHHHCCC
TGPIYYYVSQQVVYKKGNWRPRNIKNLTELDENLTIVKDSSFEGTLLKLKEKYKKLEWST
CCCEEEEECCEEEEECCCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCC
EDNTDVSELLKQVARGEIHYTLADSVELSLTQRIHPDLAVAFELTEDQPIAWFLQKTEDD
CCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCH
SLQALLIEFFGELKSSGKLALLEEKYFGHVETFDYVDTRAFIRSLESKLPKWEPLFKKYA
HHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
GDFDWRFLAALSYQESHWNPVAKSPTGVRGMMMLTLPTAQSVGVKNRLDPEESIRGGAEY
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHH
LRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQRLGGDPNSWTDVKDNLPLLR
HHHHHHHCCHHHHCHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QKRYYKYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLIQEDITVEDLTVIDVPL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECC
SSATSTTSHAVSSQ
CCCCCCCCHHCCCC
>Mature Secondary Structure 
SHIRYYRLLKSCLVISTLVFTLTGCQVESEPKTKLEQVRERGILRVSTLNNQLSYYIGS
CCHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHCCCEEEEEECCCEEEEECC
NGPTGLDYDLAKAFAEKLNVKLEITPAYTYSGLFPALNRNELDIVAANMTITPERLENFR
CCCCCCCHHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCEEEEEECCEECHHHHHCCC
TGPIYYYVSQQVVYKKGNWRPRNIKNLTELDENLTIVKDSSFEGTLLKLKEKYKKLEWST
CCCEEEEECCEEEEECCCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCC
EDNTDVSELLKQVARGEIHYTLADSVELSLTQRIHPDLAVAFELTEDQPIAWFLQKTEDD
CCCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCH
SLQALLIEFFGELKSSGKLALLEEKYFGHVETFDYVDTRAFIRSLESKLPKWEPLFKKYA
HHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
GDFDWRFLAALSYQESHWNPVAKSPTGVRGMMMLTLPTAQSVGVKNRLDPEESIRGGAEY
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCCHHHHHHHHHHH
LRRIVKRVPDSITEHEKIWFALASYNIGFGHMMDARRLTQRLGGDPNSWTDVKDNLPLLR
HHHHHHHCCHHHHCHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QKRYYKYLRYGFARGDEAQNYVENIRRYYQSIIGYEQEQANKLIQEDITVEDLTVIDVPL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECC
SSATSTTSHAVSSQ
CCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA