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The map label for this gene is guaA

Identifier: 209694327

GI number: 209694327

Start: 823131

End: 824684

Strand: Direct

Name: guaA

Synonym: VSAL_I0738

Alternate gene names: 209694327

Gene position: 823131-824684 (Clockwise)

Preceding gene: 209694326

Following gene: 209694328

Centisome position: 24.75

GC content: 41.76

Gene sequence:

>1554_bases
ATGACGACTAATATCCACGCTCAACGCATTCTTATTTTAGATTTTGGTTCTCAATACACGCAGCTTGTTGCGCGTCGTAT
CCGTGAGATTGGTGTTTACTGTGAGCTTTGGAGCTGGGATGTAGAAGAATCGGATATCCGTGACTTCAATCCTGATGGCA
TCATCTTATCTGGTGGTCCAGAAAGTGTGACAGAAGCAAATTCTCCACGAGCTCCTCAATATGTATTTGATTCTGGTGTT
CCTGTATTCGGCGTATGTTACGGCATGCAAACAATGGCAGAGCAGCTTGGCGGTAAAGTAGCAACGTCTACTGAACGTGA
GTTTGGCTATGCCGCAGTTCAAGTAACTGGCGAATCAGCGTTGTTTAAAGATCTTGAAGCAACTCAAGATGTTTGGATGA
GCCACGGTGATAAAGTTGTTGAGATCCCATCTGATTTCGTAAAAATTGCAGAGACAGAAACGTGTCCTTACGCTGCAATG
GCAAACGAAGAGAAGAAATACTATGGTGTTCAATTCCACCCAGAAGTAACACACACTAAAAATGGTCTAAAAATGCTTGA
GAACTTTGTTCTTAACGTATGTGGTTGTGAAGGTCTGTGGACTTCTGCATCAATCATTGAAGATGCCATTGCTCGCATTA
AAGAACAAGTGGGTGACGATGAAGTTATTCTTGGTCTTTCTGGTGGTGTTGATTCATCTGTGGTTGCGATGCTTGCTCAC
CGTGCGATTGGCGATAAACTAACGTGTGTATTTGTTGATAACGGTTTACTTCGTTTAAATGAAGCAGAACAAGTAATGGA
AATGTTCAGTGAAGGCTTTGGTCTTAAGATCATTCATGTTGAAGCTGAAGAACGTTTCTTAAGCGCTCTTAGTGGTGAAT
CAGATCCAGAAGCTAAGCGTAAGATCATTGGTCATGTGTTTGTTGATATTTTTGACGAAGAGTCTAAGAAATTATCAAAT
GCTAAGTGGTTAGCTCAAGGGACCATTTATCCAGATGTTATTGAGTCTGCGGCGTCTAAAACGGGTAAAGCACACGTTAT
TAAATCTCACCATAACGTTGGCGGCCTACCTGATGACATGGAAATGGGTCTTGTTGAACCGTTACGTGAATTGTTTAAAG
ATGAAGTTCGTAAGATTGGTCTAGAATTAGGTCTTCCATACAACATGCTTTATCGTCACCCGTTCCCTGGGCCCGGTCTA
GGTGTACGTGTTCTTGGTGAAGTTAAGAAAGAGTACTGTGATTTACTTCGTCGTGCTGATGCGATCTTTATTGAAGAACT
TCATAATGCTGATCTTTACAATAAAGTATCTCAAGCATTCACGGTATTCTTACCAGTACGCTCTGTTGGCGTAATGGGTG
ATGGCCGTAAATACGACTGGGTTGTCTCTCTACGTGCGGTAGAAACCATTGATTTCATGACAGCGCATTGGGCACATTTA
CCATACGATTTCTTAGGTAAAGTATCTAACCGTATTATTAATGAAGTTGGTGGTATTTCTCGTGTTGTTTACGATATTTC
AGGTAAACCACCAGCGACGATTGAGTGGGAATAA

Upstream 100 bases:

>100_bases
GCTTTGAAATATGAGAAAAATACTTTGTACCAAATTCAATTTTAAGCGTGTGGTTTTATAGTAAAACCACACTAAATAAA
TGACTTTTTGAGAAAGACTA

Downstream 100 bases:

>100_bases
TTTTCACTTAACTTGCTAATGTATATCAAATAAACAGAAAGCGCCGAAAGGCGCTTTTTTTGTTTCTATTGTAATGCAGT
TATTAAGTTTGTTAGTTATA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTTNIHAQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGPESVTEANSPRAPQYVFDSGV
PVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESALFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAM
ANEEKKYYGVQFHPEVTHTKNGLKMLENFVLNVCGCEGLWTSASIIEDAIARIKEQVGDDEVILGLSGGVDSSVVAMLAH
RAIGDKLTCVFVDNGLLRLNEAEQVMEMFSEGFGLKIIHVEAEERFLSALSGESDPEAKRKIIGHVFVDIFDEESKKLSN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGL
GVRVLGEVKKEYCDLLRRADAIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYDFLGKVSNRIINEVGGISRVVYDISGKPPATIEWE

Sequences:

>Translated_517_residues
MTTNIHAQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGPESVTEANSPRAPQYVFDSGV
PVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESALFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAM
ANEEKKYYGVQFHPEVTHTKNGLKMLENFVLNVCGCEGLWTSASIIEDAIARIKEQVGDDEVILGLSGGVDSSVVAMLAH
RAIGDKLTCVFVDNGLLRLNEAEQVMEMFSEGFGLKIIHVEAEERFLSALSGESDPEAKRKIIGHVFVDIFDEESKKLSN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGL
GVRVLGEVKKEYCDLLRRADAIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYDFLGKVSNRIINEVGGISRVVYDISGKPPATIEWE
>Mature_516_residues
TTNIHAQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGPESVTEANSPRAPQYVFDSGVP
VFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESALFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAMA
NEEKKYYGVQFHPEVTHTKNGLKMLENFVLNVCGCEGLWTSASIIEDAIARIKEQVGDDEVILGLSGGVDSSVVAMLAHR
AIGDKLTCVFVDNGLLRLNEAEQVMEMFSEGFGLKIIHVEAEERFLSALSGESDPEAKRKIIGHVFVDIFDEESKKLSNA
KWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLG
VRVLGEVKKEYCDLLRRADAIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLP
YDFLGKVSNRIINEVGGISRVVYDISGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=553, Percent_Identity=35.8047016274864, Blast_Score=301, Evalue=1e-81,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=80.9523809523809, Blast_Score=897, Evalue=0.0,
Organism=Caenorhabditis elegans, GI133901714, Length=576, Percent_Identity=34.8958333333333, Blast_Score=308, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI71992717, Length=575, Percent_Identity=34.9565217391304, Blast_Score=308, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI71992710, Length=575, Percent_Identity=34.9565217391304, Blast_Score=307, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6323873, Length=515, Percent_Identity=49.9029126213592, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322638, Length=235, Percent_Identity=28.5106382978723, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365319, Length=551, Percent_Identity=36.1161524500907, Blast_Score=292, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_ALISL (B6EGZ6)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262255.1
- ProteinModelPortal:   B6EGZ6
- GeneID:   6988634
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0738
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57422; Mature: 57291

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 86-86 ACT_SITE 173-173 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNIHAQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECCCC
ESVTEANSPRAPQYVFDSGVPVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESA
CHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHCCEEEEEEECCHH
LFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGLKMLENFVLNVCGCEGLWTSASIIEDAIARIKEQVGDDEVILGLSGGVDSSVVAMLAH
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGDKLTCVFVDNGLLRLNEAEQVMEMFSEGFGLKIIHVEAEERFLSALSGESDPEAKR
HHCCCCEEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCHHHHH
KIIGHVFVDIFDEESKKLSNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHCHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
EMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHCCHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYDFLGKVSNRIINEVGGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECC
>Mature Secondary Structure 
TTNIHAQRILILDFGSQYTQLVARRIREIGVYCELWSWDVEESDIRDFNPDGIILSGGP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEECCCC
ESVTEANSPRAPQYVFDSGVPVFGVCYGMQTMAEQLGGKVATSTEREFGYAAVQVTGESA
CHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHCCEEEEEEECCHH
LFKDLEATQDVWMSHGDKVVEIPSDFVKIAETETCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGLKMLENFVLNVCGCEGLWTSASIIEDAIARIKEQVGDDEVILGLSGGVDSSVVAMLAH
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGDKLTCVFVDNGLLRLNEAEQVMEMFSEGFGLKIIHVEAEERFLSALSGESDPEAKR
HHCCCCEEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCHHHHH
KIIGHVFVDIFDEESKKLSNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHCHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
EMGLVEPLRELFKDEVRKIGLELGLPYNMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHNADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHCCHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYDFLGKVSNRIINEVGGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA