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The map label for this gene is ispG

Identifier: 209694318

GI number: 209694318

Start: 812541

End: 813659

Strand: Direct

Name: ispG

Synonym: VSAL_I0729

Alternate gene names: 209694318

Gene position: 812541-813659 (Clockwise)

Preceding gene: 209694317

Following gene: 209694319

Centisome position: 24.44

GC content: 40.13

Gene sequence:

>1119_bases
ATGCATATAGAGTCTCCAATTAAACGTCGCCAATCTACTCGCATTTATGTGGGTAATGTTCCTATTGGTGATGGCGCACC
AATCGCTGTTCAGTCTATGACGAATACGTTAACAACTGATGTTGCTGCTACCGTTGCACAAATTAATGCACTTCAAAAAG
TCGGCGCTGATATTGTTCGTGTTTCAGTGCCAACAATGGATGCGGCAGAAGCCTTTAAGTTGATTAAACAACAGGTTTCG
GTTCCATTAGTTGCTGATATCCATTTTGATTATCGTATTGCATTAAAAGTTGCGGAATATGGCGTGGATTGCTTACGTAT
TAATCCCGGTAATATTGGTAATGAACAACGAATTCGTTCTGTTGTCGATTGTGCAAGAGATAATAATATTCCGATTCGTA
TTGGTGTGAATGGCGGTTCATTAGAAAAAGACATTCAAGCTAAATATAAAGAACCGACAGCAGAAGCATTACTAGAGTCC
GCAATGCGCCATGTCGATATATTAGATCGTATGAACTTTGACCAGTTCAAGGTCAGTGTCAAAGCTTCTGATGTCTTTTT
AGCTGTCGATTCTTATCGTTTGCTAGCAAAACAGATAGTGCAACCATTGCACCTTGGAATTACCGAGGCAGGTGGTGCTC
GTGCGGGTTCTGTTAAATCAGCCGTTGGTTTAGGTTTATTATTATCAGAAGGCATTGGCGACACATTACGTATTTCGCTA
GCGGCAGATCCTATTGAAGAGATCAAAGTTGGTTTTGACATCTTAAAATCATTACGAATTCGTTCTCGTGGTATTAATTT
TATTGCGTGCCCAACTTGTTCTCGTCAAGAGTTTGATGTTATTGCTACGGTTAATGAGCTTGAACAGCGTTTAGAAGACT
TAATCACACCAATGGATGTATCTTTGATTGGTTGTGTCGTTAATGGCCCTGGCGAGGCGGAAGTGTCTCATATGGGAATT
GCGGGAAGTTATCGTAAAAGTGCATTCTATGAAGATGGGATTCGTCAAAAAGAACGTTTTGATAACGACAATATCGTGGA
TAAGCTAGAAGCTAGGATCCGTGCAAAAGCAGCGATGTTATCAAAAGAAAATCAAATTGATATTAATCAGATCGACTGA

Upstream 100 bases:

>100_bases
TGCGGTCACATTAAGCTACAAAGGCAAACCTGTTAATTTATCTGCATATCCTGCCGGTAAAGTTGCACGACTAACTTTAC
CTCAATAATGAGTTGATTCC

Downstream 100 bases:

>100_bases
TTAATATCCAGATATAAATAAAACCCATTGGGAAGTGAACGTGGCTAAAGCAATCCAAGCAATTCGAGGCATGAATGACT
GCCTTCCAACACAGTCGCCA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTLTTDVAATVAQINALQKVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEQRIRSVVDCARDNNIPIRIGVNGGSLEKDIQAKYKEPTAEALLES
AMRHVDILDRMNFDQFKVSVKASDVFLAVDSYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRISL
AADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDVSLIGCVVNGPGEAEVSHMGI
AGSYRKSAFYEDGIRQKERFDNDNIVDKLEARIRAKAAMLSKENQIDINQID

Sequences:

>Translated_372_residues
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTLTTDVAATVAQINALQKVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEQRIRSVVDCARDNNIPIRIGVNGGSLEKDIQAKYKEPTAEALLES
AMRHVDILDRMNFDQFKVSVKASDVFLAVDSYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRISL
AADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDVSLIGCVVNGPGEAEVSHMGI
AGSYRKSAFYEDGIRQKERFDNDNIVDKLEARIRAKAAMLSKENQIDINQID
>Mature_372_residues
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTLTTDVAATVAQINALQKVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEQRIRSVVDCARDNNIPIRIGVNGGSLEKDIQAKYKEPTAEALLES
AMRHVDILDRMNFDQFKVSVKASDVFLAVDSYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRISL
AADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDVSLIGCVVNGPGEAEVSHMGI
AGSYRKSAFYEDGIRQKERFDNDNIVDKLEARIRAKAAMLSKENQIDINQID

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=82.7956989247312, Blast_Score=637, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_ALISL (B6EGY7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262246.1
- GeneID:   6986624
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0729
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40636; Mature: 40636

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTLTTDVAATVAQINALQKVGADIVR
CCCCCHHHHCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEQRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDNNIPIRIGVNGGSLEKDIQAKYKEPTAEALLESAMRHVDILDRMNFDQFKVSV
HHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVDSYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRISL
EECEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEEEHHHHHHHHHHHCCCCCC
SLIGCVVNGPGEAEVSHMGIAGSYRKSAFYEDGIRQKERFDNDNIVDKLEARIRAKAAML
EEEEEEECCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHH
SKENQIDINQID
CCCCCCCCCCCC
>Mature Secondary Structure
MHIESPIKRRQSTRIYVGNVPIGDGAPIAVQSMTNTLTTDVAATVAQINALQKVGADIVR
CCCCCHHHHCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEQRIRS
EECCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCHHHHHH
VVDCARDNNIPIRIGVNGGSLEKDIQAKYKEPTAEALLESAMRHVDILDRMNFDQFKVSV
HHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVDSYRLLAKQIVQPLHLGITEAGGARAGSVKSAVGLGLLLSEGIGDTLRISL
EECEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
AADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIATVNELEQRLEDLITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEEEHHHHHHHHHHHCCCCCC
SLIGCVVNGPGEAEVSHMGIAGSYRKSAFYEDGIRQKERFDNDNIVDKLEARIRAKAAML
EEEEEEECCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHH
SKENQIDINQID
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA